Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   BF25_RS03350 Genome accession   NZ_CP009981
Coordinates   377102..377848 (+) Length   248 a.a.
NCBI ID   WP_000487010.1    Uniprot ID   Q81LT9
Organism   Bacillus anthracis strain Ames isolate BACI008     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 372102..382848
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BF25_RS03320 (BF25_400) - 372391..374535 (+) 2145 WP_000946454.1 HD family phosphohydrolase -
  BF25_RS03325 (BF25_401) ybeY 374532..375002 (+) 471 WP_000054692.1 rRNA maturation RNase YbeY -
  BF25_RS03330 (BF25_402) - 374999..375352 (+) 354 WP_000715480.1 diacylglycerol kinase family protein -
  BF25_RS03335 (BF25_403) - 375452..375850 (+) 399 WP_001086294.1 cytidine deaminase -
  BF25_RS03340 (BF25_404) era 375843..376748 (+) 906 WP_001080240.1 GTPase Era -
  BF25_RS03345 (BF25_405) - 376925..377068 (+) 144 WP_000883924.1 YqzL family protein -
  BF25_RS03350 (BF25_406) recO 377102..377848 (+) 747 WP_000487010.1 DNA repair protein RecO Machinery gene
  BF25_RS03355 (BF25_407) - 377956..378588 (+) 633 WP_000583758.1 helix-turn-helix transcriptional regulator -
  BF25_RS03360 (BF25_408) - 378618..379430 (+) 813 WP_000368943.1 pyruvate, water dikinase regulatory protein -
  BF25_RS03375 (BF25_409) dnaG 380284..382080 (+) 1797 WP_000528217.1 DNA primase -

Sequence

Protein


Download         Length: 248 a.a.        Molecular weight: 28665.22 Da        Isoelectric Point: 8.0540

>NTDB_id=133360 BF25_RS03350 WP_000487010.1 377102..377848(+) (recO) [Bacillus anthracis strain Ames isolate BACI008]
MFQKVEGIVIRTTDYGETNKIVTIFSRELGKVSAMARGAKKPKSRLASVSQLMTHGHFLIQMGSGLGTLQQGEIISTMKE
IREDIFLTAYASFIVELTDKATEDKKHNPYLFEMLYQTLHYMCEGVDPEVLSLIYQTKMLPVLGMRPYFDTCAICHQETD
FVAFSVREGGFLCSRHAEQDQYRIPVGEAVHKLLRLFYHFDLHRLGNVSVKDSTKKQMRLVLNTYYDEYCGIYLKSRRFL
EQLDKFQI

Nucleotide


Download         Length: 747 bp        

>NTDB_id=133360 BF25_RS03350 WP_000487010.1 377102..377848(+) (recO) [Bacillus anthracis strain Ames isolate BACI008]
ATGTTTCAAAAAGTTGAGGGCATCGTTATCCGTACGACAGATTACGGAGAAACGAACAAGATTGTTACAATATTTTCAAG
AGAATTGGGTAAGGTAAGTGCAATGGCAAGAGGAGCGAAAAAACCGAAAAGTCGGTTAGCATCTGTTTCGCAACTTATGA
CACATGGTCATTTTCTTATTCAAATGGGATCTGGACTTGGAACTTTGCAACAAGGCGAGATTATTTCAACTATGAAAGAA
ATTCGCGAGGATATATTTTTAACTGCTTATGCATCATTTATTGTTGAATTAACTGATAAAGCAACAGAAGATAAAAAACA
TAATCCATATTTATTTGAAATGTTATATCAAACGTTGCATTATATGTGTGAGGGTGTTGATCCAGAAGTATTATCATTAA
TTTATCAAACGAAAATGCTTCCGGTATTAGGGATGCGCCCGTACTTTGACACATGTGCGATTTGTCATCAAGAAACAGAT
TTTGTCGCCTTCTCTGTTAGAGAGGGCGGTTTTCTGTGCTCACGTCATGCTGAGCAAGATCAGTATCGTATACCAGTGGG
GGAAGCCGTTCATAAATTATTGCGTCTTTTCTACCACTTCGATTTACATAGACTTGGCAATGTATCAGTGAAGGATAGCA
CAAAAAAACAAATGCGTTTAGTATTGAATACATATTATGATGAATATTGCGGGATTTATTTGAAATCAAGACGTTTCTTA
GAACAACTTGATAAGTTTCAAATATAA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-238)

Recombination protein O N terminal (RecO_N)

(1-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q81LT9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

55.466

99.597

0.552

Multiple sequence alignment