Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   DE8669_RS02965 Genome accession   NZ_CP012391
Coordinates   579123..581312 (+) Length   729 a.a.
NCBI ID   WP_014582388.1    Uniprot ID   -
Organism   Neisseria meningitidis strain DE8669     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 574123..586312
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DE8669_RS02950 (DE8669_0543) - 574706..575884 (+) 1179 WP_014574186.1 MacA family efflux pump subunit -
  DE8669_RS02955 (DE8669_0544) - 575950..577884 (+) 1935 WP_014582387.1 MacB family efflux pump subunit -
  DE8669_RS02960 (DE8669_0545) - 578202..578984 (-) 783 WP_002226623.1 DsbC family protein -
  DE8669_RS02965 (DE8669_0546) priA 579123..581312 (+) 2190 WP_014582388.1 primosomal protein N' Machinery gene
  DE8669_RS02970 (DE8669_0547) - 581359..582361 (-) 1003 Protein_552 NnrS family protein -
  DE8669_RS02980 (DE8669_0549) - 582409..583284 (-) 876 WP_002223993.1 IS110 family transposase -
  DE8669_RS02985 (DE8669_0550) - 583386..584289 (-) 904 Protein_554 IS5 family transposase -

Sequence

Protein


Download         Length: 729 a.a.        Molecular weight: 81121.96 Da        Isoelectric Point: 7.3905

>NTDB_id=133344 DE8669_RS02965 WP_014582388.1 579123..581312(+) (priA) [Neisseria meningitidis strain DE8669]
MIYHRIAVNVPLSDGLLTYSHSEPLPQGMRVLVPFRNKTVVGMVWETDIAPDMDTARILSIQTTFLDEPPLPESWRDLLS
FTSRYYHYPTGQAVFAALPQGLKETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGEMTMAALKQVNAQA
VKLIENWAEQGWIETTEAAKPVLRSCNGQASHSEFVLNADQQKASDAIQTALGRFQPFLLYGITGSGKTEVYFDAMAKVL
AQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLHSQMAAGRRTQDYLRAMLGQAKLVIGTRLAVFTPMDDVGLIVVDE
EHDGSFKQDNELRYHARDLAVWRAKQSGCPVVLGSATPSLESWHKVQSGAYRLLQLTERAHTAAQLPQVDILNVGRLKLD
NGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSAKMVLHQRVRQLRCHHCDHREPIPFKCPDCG
NQDLTAVGHGTQRVEETLRAFLPKAAVVRVDRDSTAHKNDWADLYRRIADNEIDILVGTQMLAKGHDFARLNLVIVLNAD
GSLYSADFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNERQMFAMPPFGFQTAVR
ADAPRVADALEFLNAAKETLAPLLPASVSQFGAAPMLMVRLAERERAQIFLESPSRQDLHRAVSLWVQVLQQNRDGKIRW
SVDVDPQEA

Nucleotide


Download         Length: 2190 bp        

>NTDB_id=133344 DE8669_RS02965 WP_014582388.1 579123..581312(+) (priA) [Neisseria meningitidis strain DE8669]
ATGATCTACCACCGTATCGCCGTAAACGTGCCGCTTTCAGACGGTCTTTTAACTTATTCCCATTCCGAGCCGCTGCCGCA
GGGAATGCGGGTGCTTGTGCCTTTTCGCAATAAAACCGTGGTCGGGATGGTGTGGGAAACGGATATTGCGCCGGATATGG
ATACGGCGCGGATTTTGAGTATTCAGACGACCTTTTTAGACGAGCCGCCGTTGCCTGAAAGCTGGCGTGATTTGCTCTCG
TTTACGTCGCGTTATTACCACTATCCGACGGGGCAGGCGGTGTTTGCCGCGCTGCCGCAGGGTTTGAAGGAAACGCGCGC
GGTGGAAATGCCGCAGCCGCCGTTGTTTTACGCGCTGAACGAAGCGGGCAGGGCGCAAACGCCGCCGCCGGCGCGGTTCA
ACAAAAAAGCGGCTTTGTGGGACGCGCTGCTGTCGGGCGAAATGACGATGGCAGCGTTGAAGCAGGTAAACGCGCAGGCG
GTGAAATTAATCGAAAATTGGGCGGAACAGGGTTGGATTGAAACGACGGAAGCGGCGAAGCCCGTTTTAAGGTCGTGTAA
TGGGCAGGCTTCGCACTCTGAATTTGTATTAAATGCTGACCAGCAGAAGGCTTCCGATGCCATTCAGACGGCATTGGGAC
GCTTCCAGCCGTTTCTGCTGTACGGCATCACCGGCAGCGGCAAGACCGAGGTGTATTTTGATGCGATGGCGAAAGTGTTG
GCTCAGGGGCGGCAGGTGTTGTTTCTGTTGCCCGAAATCAACCTCACGCCGCAGCTTTTGAAGCGGGTAGAAAACCGTTT
TGCCGACGTGCCGACCGCCGTGTTGCACAGCCAGATGGCGGCAGGCAGGCGCACGCAAGATTATTTGCGCGCGATGTTGG
GGCAGGCGAAATTAGTCATCGGCACGCGGCTGGCGGTGTTCACGCCGATGGATGATGTCGGGCTGATTGTGGTCGATGAG
GAACACGACGGCTCGTTCAAACAAGACAACGAATTACGCTACCACGCCCGCGATTTGGCGGTGTGGCGGGCGAAGCAGAG
CGGCTGCCCCGTCGTGCTGGGTAGCGCCACGCCCAGCTTGGAGAGCTGGCACAAGGTGCAAAGCGGCGCGTACCGCCTGC
TGCAACTGACCGAGCGCGCCCATACCGCTGCGCAACTGCCGCAAGTGGACATCCTCAACGTAGGCCGTCTGAAACTAGAC
AATGGCTTCTCGCCGCAAGCCTTGCAGCTTTTGAAACAGAATTTTGAAGCGGGCGGTATGTCGCTGGTGTACCTCAACCG
TCGCGGCTTCGCGCCCGCGCTGTTTTGCGGCGACTGCGGCCATACCTTCGGCTGCCCGAACTGCTCCGCCAAAATGGTGC
TGCACCAACGCGTCCGCCAACTGCGCTGCCACCACTGCGACCACCGCGAACCCATACCGTTCAAATGCCCCGACTGCGGA
AATCAAGACCTGACCGCCGTCGGACACGGCACGCAGCGCGTCGAAGAAACCCTGCGCGCCTTCCTGCCCAAGGCAGCCGT
CGTCCGCGTGGACAGGGACAGCACCGCGCACAAAAACGACTGGGCGGATTTGTACCGCCGCATCGCCGACAACGAAATCG
ACATTCTGGTCGGCACGCAGATGCTCGCCAAAGGCCATGACTTTGCGCGGCTCAACCTCGTTATCGTATTGAACGCCGAC
GGCAGCCTTTACAGCGCGGATTTCCGCGCGCCGGAAAGACTGTTCGCCGAGCTGATGCAGGTGTCCGGCAGGGCGGGGCG
CGCCGACAAACCCGGCAAGGTGTTGATACAGACCCAACTGCCCGAACATCCCGTCTTCGCCGCCGTCAAAGCGCAGGATT
ATGCCGTGTTTGCCGAAAACGAATTGAACGAACGGCAAATGTTCGCCATGCCGCCCTTCGGTTTTCAGACCGCCGTCCGC
GCGGACGCGCCGCGTGTTGCCGATGCGCTGGAATTTCTCAATGCCGCCAAAGAAACCCTCGCCCCGCTGTTGCCCGCAAG
CGTTTCCCAGTTTGGCGCCGCCCCCATGCTGATGGTGCGCCTCGCCGAACGCGAACGCGCGCAAATCTTCCTCGAATCCC
CGTCCCGACAGGATTTGCACCGTGCCGTGAGTTTGTGGGTGCAGGTGTTGCAGCAAAACCGCGACGGCAAAATTAGATGG
TCGGTGGATGTCGATCCGCAGGAGGCTTGA

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(207-362)

Primosomal protein N C-terminal domain (PriA_C)

(632-726)

3DNA-binding domain (3BD) (PriA_3primeBD)

(5-99)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(445-471)

Helicase conserved C-terminal domain (Helicase_C)

(516-588)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

37.231

100

0.38

Multiple sequence alignment