Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   AKO64_RS19275 Genome accession   NZ_CP012380
Coordinates   3679829..3680296 (+) Length   155 a.a.
NCBI ID   WP_094033060.1    Uniprot ID   -
Organism   Escherichia coli strain WAT     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3674829..3685296
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AKO64_RS19225 (AKO64_3648) rplP 3674939..3675349 (-) 411 WP_000941212.1 50S ribosomal protein L16 -
  AKO64_RS19230 (AKO64_3649) rpsC 3675362..3676063 (-) 702 WP_000529945.1 30S ribosomal protein S3 -
  AKO64_RS19235 (AKO64_3650) rplV 3676081..3676413 (-) 333 WP_000447529.1 50S ribosomal protein L22 -
  AKO64_RS19240 rpsS 3676428..3676706 (-) 279 WP_001138117.1 30S ribosomal protein S19 -
  AKO64_RS19245 (AKO64_3652) rplB 3676723..3677544 (-) 822 WP_000301864.1 50S ribosomal protein L2 -
  AKO64_RS19250 (AKO64_3653) rplW 3677562..3677864 (-) 303 WP_000617544.1 50S ribosomal protein L23 -
  AKO64_RS19255 (AKO64_3654) rplD 3677861..3678466 (-) 606 WP_000424395.1 50S ribosomal protein L4 -
  AKO64_RS19260 (AKO64_3655) rplC 3678477..3679106 (-) 630 WP_000579833.1 50S ribosomal protein L3 -
  AKO64_RS19265 (AKO64_3656) rpsJ 3679139..3679450 (-) 312 WP_001181004.1 30S ribosomal protein S10 -
  AKO64_RS19275 (AKO64_3657) pilD 3679829..3680296 (+) 468 WP_094033060.1 A24 family peptidase Machinery gene
  AKO64_RS19280 (AKO64_3658) bfr 3680293..3680769 (-) 477 WP_000675515.1 bacterioferritin -
  AKO64_RS19285 (AKO64_3659) bfd 3680842..3681036 (-) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  AKO64_RS19295 (AKO64_3660) tuf 3681219..3682403 (-) 1185 WP_000031783.1 elongation factor Tu -
  AKO64_RS19300 (AKO64_3661) fusA 3682474..3684588 (-) 2115 WP_000124700.1 elongation factor G -
  AKO64_RS19305 (AKO64_3662) rpsG 3684685..3685155 (-) 471 WP_001138043.1 30S ribosomal protein S7 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16926.03 Da        Isoelectric Point: 8.1296

>NTDB_id=133296 AKO64_RS19275 WP_094033060.1 3679829..3680296(+) (pilD) [Escherichia coli strain WAT]
MDATLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFSQVCNPDGLADALWGAIIGYGTFAVTYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=133296 AKO64_RS19275 WP_094033060.1 3679829..3680296(+) (pilD) [Escherichia coli strain WAT]
ATGGACGCTACCCTTCCCTTTCTGATTTTGTACGCTTGTTTATCTGTCCTACTTTTTTTATGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTCTCAGGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCACTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACCTGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTGTTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGTTGGGATAGTTTGCTGGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

38.095

94.839

0.361