Detailed information NTG0133216

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: BF28_RS05005
• Genome accession: NZ_CP009968
• Coordinates: 486497..487846 (+)
• Length: 449 a.a.
• NCBI ID: WP_000225365.1
• Uniprot ID: -
• Organism: Bacillus cereus E33L
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 481497..492846

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BF28_RS04980 (BF28_493)	-	482599..483234 (-)	636	WP_000926685.1	YigZ family protein	-
BF28_RS04985 (BF28_494)	-	483474..484316 (+)	843	WP_000844770.1	DegV family protein	-
BF28_RS04995 (BF28_495)	-	484479..484793 (+)	315	WP_000400857.1	winged helix-turn-helix transcriptional regulator	-
BF28_RS05000 (BF28_496)	-	485047..486369 (+)	1323	WP_011199072.1	NlpC/P60 family protein	-
BF28_RS05005 (BF28_497)	comFA	486497..487846 (+)	1350	WP_000225365.1	ATP-dependent helicase ComFA	Machinery gene
BF28_RS05010 (BF28_498)	comFC	487846..488550 (+)	705	WP_011199070.1	comF operon protein ComFC	-
BF28_RS05015 (BF28_499)	cspC	488677..488874 (+)	198	WP_001990088.1	cold shock protein CspC	-
BF28_RS05020 (BF28_500)	hpf	489198..489740 (+)	543	WP_000671189.1	ribosome hibernation-promoting factor, HPF/YfiA family	-
BF28_RS05025 (BF28_501)	secA	490017..492524 (+)	2508	WP_000579383.1	preprotein translocase subunit SecA	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 51177.91 Da
• Isoelectric Point: 9.7732

>NTDB_id=133216 BF28_RS05005 WP_000225365.1 486497..487846(+) (comFA) [Bacillus cereus E33L]
MVAGKQLLLEELSSDLRRELSDLKKKGEIICVQGIIKKASKYICQRCGNIEQRLFASFLCKRCSKVCTYCRKCITMGRVS
ECAVLVRGIHERKGERELHSLQWKGSLSLGQELAAQGVIEAIKQKESFFIWAVCGAGKTEMLFYGIEEALQKGERVCIAT
PRTDVVLELAPRLQEVFPSINVAALYGGSVDREKDAALVVATTHQLLRYYRAFHVMIVDEIDAFPYHADQMLQYAVQQAM
KEKAARIYLTATPDEKWKRNFRRGKQKGIIVSGRYHRHPLPVPLFSWCGNWKKSLHHKKIPRVLLQWLKMYVNKKYPIFL
FVPHVRYIEEIGLLLKGLDHRIDGVHAEDPMRKEKVAAFRKGDIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEEPYGEVVYFHYGKTESMVRAKKHIQSMNKSAKEQGLID

Nucleotide

• Length: 1350 bp

>NTDB_id=133216 BF28_RS05005 WP_000225365.1 486497..487846(+) (comFA) [Bacillus cereus E33L]
ATGGTTGCTGGAAAGCAGTTACTATTAGAAGAACTCTCTTCAGATTTACGGAGAGAATTAAGTGATTTGAAAAAGAAGGG
AGAGATCATATGTGTACAAGGCATAATAAAGAAGGCTTCTAAATATATATGTCAGCGCTGCGGGAATATAGAGCAGCGGC
TATTTGCATCATTTTTATGTAAAAGGTGCAGTAAAGTATGTACGTATTGCCGGAAGTGTATAACGATGGGAAGAGTTAGT
GAATGTGCTGTACTTGTTCGTGGGATTCACGAAAGAAAAGGAGAAAGGGAATTACATTCGTTACAGTGGAAAGGGAGTTT
GTCTCTTGGTCAGGAGCTGGCGGCGCAAGGTGTTATAGAAGCTATTAAGCAGAAAGAATCGTTTTTTATTTGGGCTGTGT
GCGGGGCTGGAAAAACAGAAATGTTATTTTACGGTATAGAAGAGGCACTTCAAAAAGGAGAAAGAGTGTGTATCGCAACG
CCAAGGACGGACGTTGTACTGGAATTAGCACCGAGATTACAAGAAGTGTTTCCAAGTATAAATGTAGCTGCTTTATACGG
AGGGAGTGTAGATCGTGAAAAAGATGCAGCGTTAGTCGTTGCAACGACTCACCAACTGTTACGTTACTATAGAGCGTTTC
ATGTAATGATTGTAGATGAGATTGATGCCTTCCCGTATCATGCGGATCAAATGTTACAGTATGCAGTGCAGCAAGCGATG
AAAGAGAAAGCAGCGCGTATTTATTTAACTGCAACCCCTGATGAAAAGTGGAAGCGCAATTTCAGAAGGGGGAAACAAAA
AGGTATCATTGTCTCTGGACGATATCACCGTCATCCGTTACCAGTTCCTCTATTTAGTTGGTGCGGAAATTGGAAGAAAA
GCCTCCATCACAAAAAAATTCCTCGTGTGTTACTACAATGGTTAAAAATGTACGTAAACAAAAAATATCCTATTTTTTTA
TTTGTTCCTCATGTGCGATATATAGAAGAAATAGGCCTGTTATTGAAAGGGTTGGATCATAGAATCGATGGCGTACATGC
AGAAGATCCGATGAGAAAAGAAAAAGTGGCAGCGTTTAGAAAGGGAGACATTCCGTTATTAGTTACAACGACAATTTTAG
AAAGAGGGGTAACTGTGAAGAACTTACAAGTGGCTGTGTTAGGGGCTGAAGAAGAAATCTTTTCAGAAAGTGCGCTCGTA
CAAATTGCAGGCCGAGCAGGTCGTAGTTTTGAAGAACCGTATGGTGAGGTTGTTTATTTTCATTACGGTAAGACAGAGTC
GATGGTACGTGCGAAAAAACATATTCAAAGTATGAACAAAAGTGCGAAAGAACAAGGATTAATTGATTAA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (108-254)

• Helicase conserved C-terminal domain (Helicase_C) (312-408)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	52.871	93.096	0.492