Detailed information    

insolico Bioinformatically predicted

Overview

Name   recF   Type   Machinery gene
Locus tag   BF28_RS03450 Genome accession   NZ_CP009968
Coordinates   156527..157654 (-) Length   375 a.a.
NCBI ID   WP_000470747.1    Uniprot ID   -
Organism   Bacillus cereus E33L     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 151527..162654
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BF28_RS03440 (BF28_186) gyrA 152006..154477 (-) 2472 WP_001282855.1 DNA gyrase subunit A -
  BF28_RS03445 (BF28_187) gyrB 154566..156488 (-) 1923 WP_000435986.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  BF28_RS03450 (BF28_188) recF 156527..157654 (-) 1128 WP_000470747.1 DNA replication/repair protein RecF Machinery gene
  BF28_RS03455 (BF28_189) yaaA 157667..157879 (-) 213 WP_000821367.1 S4 domain-containing protein YaaA -
  BF28_RS03460 (BF28_190) dnaN 158007..159146 (-) 1140 WP_001212891.1 DNA polymerase III subunit beta -
  BF28_RS03465 (BF28_191) dnaA 159325..160665 (-) 1341 WP_000428021.1 chromosomal replication initiator protein DnaA -
  BF28_RS03470 (BF28_192) rpmH 161231..161365 (+) 135 WP_000831901.1 50S ribosomal protein L34 -
  BF28_RS03475 (BF28_193) rnpA 161442..161801 (+) 360 WP_000726628.1 ribonuclease P protein component -
  BF28_RS03480 (BF28_194) spoIIIJ 161841..162608 (+) 768 WP_000727748.1 YidC family membrane integrase SpoIIIJ -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 43362.67 Da        Isoelectric Point: 6.6001

>NTDB_id=133210 BF28_RS03450 WP_000470747.1 156527..157654(-) (recF) [Bacillus cereus E33L]
MFISEIQLKNYRNYEKLELSFEDKVNVIIGENAQGKTNLMEAIYVLAMAKSHRTSNDRELIRWDEDFGQIKGKLQKRNSS
LSLELNISKKGKKAKLNQLEQQKLSQYIGVMNVVMFAPEDLNLVKGSPQVRRRFLDMELGQIAPVYLYELSQYQKVLTQR
NHLLKKMQGNSKNEETMLDVFTLQLIEHGAKILQKRFEFLHLLQEWAAPIHRGISRGLEELEIVYKPSVDVSESMDLSKI
KEVYYESFQSVKQREIFRGTTLLGPHRDDLQFFVNSKNVQVFGSQGQQRTTALSLKLAEIELIYSEVKEYPILLLDDVLS
ELDDYRQSHLLNTIQGKVQTFVTTTSVDGIEHETLKEAKTIHVTNGTVDCEIDRE

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=133210 BF28_RS03450 WP_000470747.1 156527..157654(-) (recF) [Bacillus cereus E33L]
TTGTTTATTTCAGAAATACAATTAAAAAACTATCGCAATTATGAAAAGTTAGAGCTTTCCTTTGAAGATAAGGTAAATGT
AATTATCGGCGAGAATGCGCAAGGGAAAACGAATTTGATGGAAGCTATTTATGTTTTAGCGATGGCGAAATCTCATAGAA
CCTCTAATGATCGCGAGCTTATCCGTTGGGATGAAGATTTCGGTCAAATTAAGGGAAAGTTACAAAAGAGAAATAGTTCT
TTGTCTTTGGAATTAAATATCTCGAAAAAAGGTAAAAAGGCAAAATTAAATCAACTTGAACAACAAAAATTGAGTCAATA
TATTGGCGTAATGAATGTTGTCATGTTTGCCCCAGAAGATTTAAATCTTGTAAAAGGAAGCCCTCAAGTAAGAAGACGCT
TTTTAGATATGGAATTAGGCCAAATAGCTCCGGTCTATTTGTATGAATTGAGTCAATATCAAAAGGTGCTCACACAACGA
AATCACTTGCTGAAAAAGATGCAAGGGAATAGTAAGAATGAGGAAACGATGTTGGATGTATTTACACTTCAACTTATTGA
GCATGGTGCAAAGATATTGCAAAAGCGTTTTGAATTTTTGCATTTATTACAAGAATGGGCAGCTCCAATTCATCGCGGTA
TAAGCCGTGGATTAGAAGAATTAGAAATTGTCTATAAACCAAGTGTAGATGTATCAGAATCAATGGATTTGTCGAAAATA
AAAGAAGTATACTATGAAAGTTTTCAATCTGTGAAACAACGTGAAATTTTCCGTGGTACGACTTTACTTGGTCCTCATCG
TGATGATTTACAATTCTTCGTTAATAGTAAAAATGTTCAAGTCTTTGGTTCACAAGGACAACAACGAACGACCGCACTGT
CCCTAAAATTAGCTGAAATTGAATTGATTTACTCAGAAGTTAAGGAATATCCGATCCTTTTATTGGATGATGTATTATCA
GAATTAGATGATTATCGTCAATCACATCTGTTAAATACAATTCAAGGAAAAGTGCAAACATTTGTGACAACGACGAGTGT
CGACGGAATTGAACACGAAACATTAAAAGAAGCGAAAACAATTCATGTAACGAACGGCACGGTAGATTGTGAAATAGATA
GGGAATAA

Domains

Predicted by InterproScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(2-348)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recF Bacillus subtilis subsp. subtilis str. 168

66.576

98.133

0.653

Multiple sequence alignment