Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   BF28_RS02950 Genome accession   NZ_CP009968
Coordinates   61994..63370 (-) Length   458 a.a.
NCBI ID   WP_001085207.1    Uniprot ID   A0A2B4FB19
Organism   Bacillus cereus E33L     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 56994..68370
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BF28_RS02930 (BF28_81) ispF 58358..58834 (-) 477 WP_000488386.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  BF28_RS02935 (BF28_82) ispD 58949..59629 (-) 681 WP_000288292.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  BF28_RS02940 (BF28_83) - 59646..60755 (-) 1110 WP_000919684.1 PIN/TRAM domain-containing protein -
  BF28_RS02945 (BF28_84) disA 60917..61990 (-) 1074 WP_000392168.1 DNA integrity scanning diadenylate cyclase DisA -
  BF28_RS02950 (BF28_85) radA 61994..63370 (-) 1377 WP_001085207.1 DNA repair protein RadA Machinery gene
  BF28_RS02955 (BF28_86) clpC 63467..65902 (-) 2436 WP_000971192.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  BF28_RS02960 (BF28_87) - 65925..66989 (-) 1065 WP_000050831.1 protein arginine kinase -
  BF28_RS02965 (BF28_88) - 66994..67542 (-) 549 WP_000128391.1 UvrB/UvrC motif-containing protein -
  BF28_RS02970 (BF28_89) ctsR 67702..68163 (-) 462 WP_001244563.1 transcriptional regulator CtsR -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49814.39 Da        Isoelectric Point: 7.1421

>NTDB_id=133206 BF28_RS02950 WP_001085207.1 61994..63370(-) (radA) [Bacillus cereus E33L]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQSEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVNGSNLFVVSETDLQRIA
AHIEEVNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=133206 BF28_RS02950 WP_001085207.1 61994..63370(-) (radA) [Bacillus cereus E33L]
ATGGCTAAAAAGAAAACAAAATTCACATGTCAAGAGTGTGGGTATCAGTCACCGAAATATATGGGGAAATGCCCTGGATG
TGGTCAATGGAATACGCTTGTTGAAGAGATGGAACCGGTTGTATCATCAAGGCGCCTTAATTATGCGAATGCAATTCAAT
CGGAAGTAACAAAACCAAGACGCCTAACAGAAGTAGAAACAAAGTCTGAGGCGCGTATTGAAACGAAATTTCAAGAATTT
AATCGTGTACTTGGTGGCGGGATTGTAGATGGTTCTTTAGTACTTATTGGTGGAGACCCTGGGATTGGAAAATCAACGTT
ACTATTACAAATATCATCGCAATTAGCGGATTCTTCATATGATGTACTATATATATCGGGTGAAGAATCAGCAAAACAGA
TTAAACTGCGTGCGGATCGTTTACATGTAAATGGTAGTAATCTATTTGTTGTGTCAGAGACGGATTTACAGCGAATTGCG
GCACACATTGAAGAAGTGAATCCAGCCTTTGTTGTTATTGACTCTATTCAAACAATACATCTACCTGAAGTAACATCAGC
GCCGGGAAGTGTGGCACAAGTTCGTGAATGTACAGCTGAATTAATGAAACTTGCAAAAACGAAAGGAATCCCTATTTTTA
TCGTTGGACATGTGACAAAAGAGGGAGCAATTGCAGGACCTCGTATGTTAGAACATATGGTCGATGCAGTTCTTTATTTT
GAAGGAGACCGTCATCATACATACCGTATTTTGCGAGCAGTAAAGAATCGCTTTGGTTCTACGAATGAAATGGGTATTTT
TGAAATGAAGGAACTTGGTCTTGCAGAAGTATTAAACCCTTCTGAGATCTTCCTTGAGGAAAGACCAGTTGGGGTTGCGG
GATCAACAGTGGTTGCTTCAATGGAAGGAACAAGACCAGTTTTAGTAGAAATACAAGCATTAATCTCCCCTACTAGTTTT
GGAAACCCTCGAAGAATGGCAACGGGAATTGATCATAACCGTGTATCGCTTATTATGGCGGTGTTAGAAAAAAGAACAGG
CTTATTATTGCAAAATCAGGACGCATATTTAAAAGTAGCAGGTGGTTTGAAGTTAGATGAACCAGCTATTGATTTAGCTG
TCGCTTTAAGTATAGCTTCAAGTTTCAGAGATAAATCTACAGCACCAACCGATGCAGTAATAGGAGAAGTAGGATTAACT
GGAGAAATAAGAAGAGTATCAAGAATTGAACAACGTGTACAAGAAGCAGCTAAATTAGGATTTCAACGTGCTATTATTCC
TAGAAAAAATTTGGGAGGATGGACAATTCCGGATGGGATTGAGGTAGTTGGTGTATCTAATTTAGGAGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(60-222)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2B4FB19

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.728

100

0.749
  radA Streptococcus pneumoniae Rx1

63.797

98.908

0.631
  radA Streptococcus pneumoniae D39

63.797

98.908

0.631
  radA Streptococcus pneumoniae R6

63.797

98.908

0.631
  radA Streptococcus pneumoniae TIGR4

63.797

98.908

0.631
  radA Streptococcus mitis SK321

63.797

98.908

0.631
  radA Streptococcus mitis NCTC 12261

63.576

98.908

0.629

Multiple sequence alignment