Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BG04_RS04215 Genome accession   NZ_CP009920
Coordinates   670616..670990 (+) Length   124 a.a.
NCBI ID   WP_230586599.1    Uniprot ID   -
Organism   Priestia megaterium NBRC 15308 = ATCC 14581     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 665616..675990
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BG04_RS04195 (BG04_786) - 666109..666780 (-) 672 WP_034649601.1 response regulator transcription factor -
  BG04_RS04200 (BG04_787) - 667106..667924 (+) 819 WP_013058559.1 squalene/phytoene synthase family protein -
  BG04_RS04205 (BG04_788) - 667960..669909 (-) 1950 WP_034649599.1 FUSC family protein -
  BG04_RS04210 (BG04_789) - 670066..670326 (-) 261 WP_013058561.1 metal-sensitive transcriptional regulator -
  BG04_RS04215 (BG04_790) nucA/comI 670616..670990 (+) 375 WP_230586599.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  BG04_RS04220 (BG04_791) comJ 671026..671433 (+) 408 WP_016765176.1 competence protein ComJ -
  BG04_RS04225 (BG04_792) helD 671462..673780 (-) 2319 WP_034649592.1 RNA polymerase recycling motor HelD -
  BG04_RS31630 (BG04_793) - 674025..674150 (-) 126 WP_016765178.1 hypothetical protein -
  BG04_RS04230 (BG04_794) - 674290..674805 (-) 516 WP_034655000.1 enoyl-CoA hydratase/isomerase family protein -
  BG04_RS04235 (BG04_795) - 675047..675415 (+) 369 WP_013084552.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 124 a.a.        Molecular weight: 13659.93 Da        Isoelectric Point: 4.2601

>NTDB_id=132943 BG04_RS04215 WP_230586599.1 670616..670990(+) (nucA/comI) [Priestia megaterium NBRC 15308 = ATCC 14581]
MGHGAFLAPEKTEAEVNYDATLYFPLDRYPETGDHIRDAIAAGHSSVCTIDRDGAEANREESLKGYPTKIGYDRDEWPMA
MCEEGGAGADIRYISPSDNRGAGSWVGNQLEQYPDGTRVQFIVQ

Nucleotide


Download         Length: 375 bp        

>NTDB_id=132943 BG04_RS04215 WP_230586599.1 670616..670990(+) (nucA/comI) [Priestia megaterium NBRC 15308 = ATCC 14581]
ATGGGACATGGAGCTTTTTTAGCTCCCGAAAAAACGGAAGCTGAAGTAAATTATGATGCGACACTTTATTTTCCGTTAGA
CCGATATCCTGAAACGGGTGATCATATTCGTGACGCGATTGCCGCTGGGCACTCTTCCGTTTGCACAATTGATCGCGACG
GTGCTGAAGCGAACCGAGAAGAATCCCTGAAAGGATATCCGACAAAAATAGGTTATGACCGTGATGAATGGCCAATGGCT
ATGTGCGAAGAAGGCGGAGCAGGAGCTGACATTCGCTATATTTCGCCTTCTGATAACCGTGGAGCCGGCTCATGGGTTGG
AAATCAGTTAGAACAGTATCCAGATGGTACACGCGTACAATTTATCGTTCAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(30-120)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

67.227

95.968

0.645

Multiple sequence alignment