Detailed information NTG0132441

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: XFPR_RS06115
• Genome accession: NZ_CP009826
• Coordinates: 1363690..1364820 (+)
• Length: 376 a.a.
• NCBI ID: WP_010894128.1
• Uniprot ID: Q9PCX2
• Organism: Xylella fastidiosa strain Pr8x
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1358690..1369820

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
XFPR_RS06100 (XFPR_06055)	-	1358912..1359736 (+)	825	WP_023907665.1	glycosyltransferase family 2 protein	-
XFPR_RS06105 (XFPR_06060)	-	1360400..1361227 (-)	828	WP_031336660.1	glycosyltransferase family 2 protein	-
XFPR_RS06110 (XFPR_06070)	pilT	1362490..1363524 (+)	1035	WP_004084948.1	type IV pilus twitching motility protein PilT	Machinery gene
XFPR_RS06115 (XFPR_06075)	pilU	1363690..1364820 (+)	1131	WP_010894128.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
XFPR_RS06120 (XFPR_06080)	-	1365337..1366350 (-)	1014	WP_229716473.1	YafY family protein	-
XFPR_RS06125 (XFPR_06085)	-	1367194..1368003 (+)	810	WP_031336664.1	DUF481 domain-containing protein	-
XFPR_RS06130 (XFPR_06090)	hemC	1368057..1368974 (-)	918	WP_010894123.1	hydroxymethylbilane synthase	-
XFPR_RS06135 (XFPR_06095)	-	1368991..1369749 (-)	759	WP_010894122.1	LytTR family DNA-binding domain-containing protein	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42476.67 Da
• Isoelectric Point: 6.5027

>NTDB_id=132441 XFPR_RS06115 WP_010894128.1 1363690..1364820(+) (pilU) [Xylella fastidiosa strain Pr8x]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITN
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHERCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DRIINFFPEERRSQLLTDLSLNLKGIIAQQLIPSPDGKERCLAAEVLRDSRLVQDYIRVGEIHKLKEVMQNPNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGVEIAEMS

Nucleotide

• Length: 1131 bp

>NTDB_id=132441 XFPR_RS06115 WP_010894128.1 1363690..1364820(+) (pilU) [Xylella fastidiosa strain Pr8x]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAAGTCCATGAATGCAACTTCGCAATTGACATCACCAAC
ATTGGTCGCTTCCGCGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTGGGTG
CCACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAGATGCATCATCACTCAACGCGAAATAGGTACCGACACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTACGCCAAGCCCCAGATGTGATTGTGATTGGTGAGGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCGTTCTCAGAAACCGGCCATCTGGTCATGTGTACCCTACATGCCAACAACGCCAATCAAGCAATA
GACCGCATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGACTGATCTGTCGCTGAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTCCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGTCGATTAGTAC
AGGACTACATACGCGTAGGCGAGATCCATAAGCTCAAAGAAGTCATGCAAAACCCCAACGATCTTGGAATGACTACATTC
GATCAAAGCTTGGTCGAACTCTATCATAGCGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGAAAAGACGCCAAAACTCTGAGTCAAGGATTCGATGGAGTAGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-272)

3D structure

• AlphaFold DB: Q9PCX2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.58	97.606	0.601
pilU	Acinetobacter baylyi ADP1	59.887	94.149	0.564
pilU	Vibrio cholerae strain A1552	51.124	94.681	0.484
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.209	96.809	0.399