Detailed information
Bioinformatically predicted
Overview
| Name | nucA/comI | Type | Machinery gene |
| Locus tag | OB04_RS13150 | Genome accession | NZ_CP009796 |
| Coordinates | 2530872..2531282 (-) | Length | 136 a.a. |
| NCBI ID | WP_038429332.1 | Uniprot ID | - |
| Organism | Bacillus subtilis strain SG6 | ||
| Function | cleavage of dsDNA into ssDNA (predicted from homology) DNA processing |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| Prophage | 2526237..2555424 | 2530872..2531282 | within | 0 |
Gene organization within MGE regions
Location: 2526237..2555424
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| OB04_RS13120 (OB04_02571) | yqeF | 2526404..2527135 (-) | 732 | WP_015714294.1 | SGNH/GDSL hydrolase family protein | - |
| OB04_RS13130 (OB04_02572) | cwlH | 2527388..2528140 (-) | 753 | WP_038429329.1 | N-acetylmuramoyl-L-alanine amidase CwlH | - |
| OB04_RS13135 (OB04_02573) | yqeD | 2528327..2528953 (+) | 627 | WP_038429330.1 | TVP38/TMEM64 family protein | - |
| OB04_RS13140 (OB04_02574) | gnd | 2528972..2529865 (-) | 894 | WP_038429331.1 | phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) | - |
| OB04_RS13145 (OB04_02575) | yqeB | 2530117..2530839 (+) | 723 | WP_010886572.1 | hypothetical protein | - |
| OB04_RS13150 (OB04_02576) | nucA/comI | 2530872..2531282 (-) | 411 | WP_038429332.1 | sporulation-specific Dnase NucB | Machinery gene |
| OB04_RS13155 (OB04_02577) | sigK | 2531478..2532206 (+) | 729 | WP_013308023.1 | RNA polymerase sporulation sigma factor SigK | - |
| OB04_RS22445 | - | 2532206..2532298 (+) | 93 | Protein_2556 | hypothetical protein | - |
| OB04_RS21735 (OB04_02578) | - | 2532281..2532421 (-) | 141 | Protein_2557 | recombinase | - |
| OB04_RS13170 (OB04_02579) | yrkL | 2532966..2533490 (-) | 525 | WP_038429334.1 | NAD(P)H-dependent oxidoreductase | - |
| OB04_RS13175 (OB04_02580) | yrkK | 2533738..2534212 (-) | 475 | Protein_2559 | HXXEE domain-containing protein | - |
| OB04_RS13180 (OB04_02582) | - | 2534716..2536095 (-) | 1380 | WP_038429335.1 | aspartate aminotransferase family protein | - |
| OB04_RS13185 (OB04_02583) | - | 2536312..2537178 (+) | 867 | WP_038429336.1 | MurR/RpiR family transcriptional regulator | - |
| OB04_RS13190 (OB04_02584) | - | 2537912..2538253 (-) | 342 | WP_038429337.1 | MmcQ/YjbR family DNA-binding protein | - |
| OB04_RS13195 (OB04_02585) | - | 2538279..2539604 (-) | 1326 | WP_038429338.1 | replication-associated recombination protein A | - |
| OB04_RS13200 (OB04_02586) | - | 2539749..2540510 (-) | 762 | WP_250540521.1 | helix-turn-helix transcriptional regulator | - |
| OB04_RS13205 (OB04_02587) | - | 2541011..2541856 (+) | 846 | WP_038429340.1 | radical SAM protein | - |
| OB04_RS13210 (OB04_02588) | - | 2541907..2542503 (+) | 597 | WP_038429341.1 | 3'-5' exonuclease | - |
| OB04_RS13215 (OB04_02589) | - | 2542798..2544261 (+) | 1464 | WP_052124609.1 | carboxylesterase family protein | - |
| OB04_RS13220 (OB04_02591) | - | 2544612..2545739 (-) | 1128 | WP_038429342.1 | Rap family tetratricopeptide repeat protein | - |
| OB04_RS22740 (OB04_02592) | - | 2545925..2547850 (+) | 1926 | WP_038429343.1 | T7SS effector LXG polymorphic toxin | - |
| OB04_RS13230 (OB04_02593) | - | 2547864..2548151 (+) | 288 | WP_038429344.1 | hypothetical protein | - |
| OB04_RS22580 | - | 2548455..2548541 (+) | 87 | WP_072176383.1 | hypothetical protein | - |
| OB04_RS13235 (OB04_02594) | - | 2548554..2548994 (+) | 441 | WP_014480332.1 | SMI1/KNR4 family protein | - |
| OB04_RS13240 (OB04_02595) | - | 2549093..2549545 (+) | 453 | WP_038429345.1 | SMI1/KNR4 family protein | - |
| OB04_RS13245 (OB04_02596) | cdiI | 2549642..2550001 (+) | 360 | WP_038429346.1 | ribonuclease toxin immunity protein CdiI | - |
| OB04_RS13250 (OB04_02597) | - | 2550054..2550389 (+) | 336 | WP_038429347.1 | hypothetical protein | - |
| OB04_RS13255 (OB04_02598) | - | 2550542..2550958 (-) | 417 | WP_038429348.1 | hypothetical protein | - |
| OB04_RS13260 (OB04_02599) | - | 2551160..2551906 (-) | 747 | WP_350308192.1 | N-acetylmuramoyl-L-alanine amidase | - |
| OB04_RS21740 | - | 2551952..2552092 (-) | 141 | Protein_2578 | phage holin family protein | - |
| OB04_RS13265 (OB04_02600) | - | 2552119..2552304 (-) | 186 | Protein_2579 | phage terminase large subunit | - |
| OB04_RS13270 | terS | 2552301..2553009 (-) | 709 | Protein_2580 | phage terminase small subunit | - |
| OB04_RS22585 | - | 2553023..2553091 (-) | 69 | WP_250540529.1 | phage terminase small subunit-related protein | - |
| OB04_RS21745 | - | 2553273..2553359 (+) | 87 | WP_073991391.1 | YjcZ family sporulation protein | - |
| OB04_RS21750 (OB04_02603) | - | 2553400..2553492 (+) | 93 | WP_080019512.1 | YjcZ family sporulation protein | - |
| OB04_RS21755 (OB04_02604) | cotD | 2553678..2553893 (-) | 216 | WP_076458314.1 | spore coat protein CotD | - |
| OB04_RS13275 (OB04_02605) | yqaQ | 2554450..2554860 (-) | 411 | WP_052124610.1 | sigma factor-like helix-turn-helix DNA-binding protein | - |
| OB04_RS13280 (OB04_02606) | - | 2555158..2555424 (+) | 267 | WP_029318115.1 | hypothetical protein | - |
Sequence
Protein
Download Length: 136 a.a. Molecular weight: 14891.88 Da Isoelectric Point: 5.1854
>NTDB_id=132349 OB04_RS13150 WP_038429332.1 2530872..2531282(-) (nucA/comI) [Bacillus subtilis strain SG6]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSSPDGTRVLFIVQ
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSSPDGTRVLFIVQ
Nucleotide
Download Length: 411 bp
>NTDB_id=132349 OB04_RS13150 WP_038429332.1 2530872..2531282(-) (nucA/comI) [Bacillus subtilis strain SG6]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTCCCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTCCCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA
3D structure
| Source | ID | Structure |
|---|
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| nucA/comI | Bacillus subtilis subsp. subtilis str. 168 |
61.739 |
84.559 |
0.522 |