Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   FORC6_RS01610 Genome accession   NZ_CP009765
Coordinates   335215..336231 (+) Length   338 a.a.
NCBI ID   WP_005496568.1    Uniprot ID   A0A3E1IL27
Organism   Vibrio parahaemolyticus strain FORC_006     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 330215..341231
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC6_RS01595 (FORC6_0268) - 330639..331367 (-) 729 WP_015297397.1 redoxin family protein -
  FORC6_RS01600 (FORC6_0269) oxyR 331538..332446 (+) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  FORC6_RS01605 (FORC6_0270) - 332502..335072 (-) 2571 WP_005496567.1 penicillin-binding protein 1A -
  FORC6_RS01610 (FORC6_0271) pilM 335215..336231 (+) 1017 WP_005496568.1 type IV pilus biogenesis protein PilM Machinery gene
  FORC6_RS01615 (FORC6_0272) pilN 336215..336793 (+) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  FORC6_RS01620 (FORC6_0273) pilO 336786..337379 (+) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  FORC6_RS01625 (FORC6_0274) pilP 337369..337884 (+) 516 WP_060657245.1 pilus assembly protein PilP Machinery gene
  FORC6_RS01630 (FORC6_0275) pilQ 337916..339664 (+) 1749 WP_025612022.1 type IV pilus secretin PilQ Machinery gene
  FORC6_RS01635 (FORC6_0276) aroK 339853..340371 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37762.28 Da        Isoelectric Point: 6.8323

>NTDB_id=131983 FORC6_RS01610 WP_005496568.1 335215..336231(+) (pilM) [Vibrio parahaemolyticus strain FORC_006]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=131983 FORC6_RS01610 WP_005496568.1 335215..336231(+) (pilM) [Vibrio parahaemolyticus strain FORC_006]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTATTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTCGTACGCCTTCGTGCGGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAAGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGTTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATCTCAATGGGGACGTCACAAGAAGACTTACTTTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACGTCGATCAACGGTTCGCAAAACATTAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTGTCGCATCAGTTGGCATTGGAGTGTGAGTTGTTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGCCGAGCGGCCGATTGGCAACATTTTTCGACGGCTGCA
GGGCTGGCGGTGCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTGTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3E1IL27

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

85.207

100

0.852
  pilM Vibrio cholerae strain A1552

48.368

99.704

0.482

Multiple sequence alignment