Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   BWEI_RS16265 Genome accession   NZ_CP009746
Coordinates   3276404..3277006 (+) Length   200 a.a.
NCBI ID   WP_002030800.1    Uniprot ID   A0A366GKC2
Organism   Bacillus mycoides strain WSBC10204     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3271404..3282006
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BWEI_RS16230 (bwei_3433) - 3271829..3272383 (-) 555 WP_002030808.1 DUF1273 domain-containing protein -
  BWEI_RS16235 (bwei_3434) - 3272440..3272850 (-) 411 WP_002126485.1 CotD family spore coat protein -
  BWEI_RS16240 (bwei_3435) - 3273007..3273669 (-) 663 WP_033708939.1 YppG family protein -
  BWEI_RS16245 (bwei_3436) - 3273847..3274038 (+) 192 WP_002030817.1 YppF family protein -
  BWEI_RS16250 (bwei_3437) - 3274064..3274408 (-) 345 WP_002011864.1 YppE family protein -
  BWEI_RS16255 (bwei_3438) - 3274424..3274666 (-) 243 WP_002030820.1 hypothetical protein -
  BWEI_RS31040 - 3274785..3274931 (+) 147 WP_306302660.1 phage portal protein -
  BWEI_RS16260 (bwei_3439) - 3275275..3276300 (-) 1026 WP_002030803.1 DUF2515 domain-containing protein -
  BWEI_RS16265 (bwei_3440) recU 3276404..3277006 (+) 603 WP_002030800.1 Holliday junction resolvase RecU Machinery gene
  BWEI_RS16270 (bwei_3441) - 3277073..3279808 (+) 2736 WP_002030798.1 penicillin-binding protein 1A -
  BWEI_RS16275 (bwei_3442) - 3279835..3280353 (-) 519 WP_002030796.1 YpoC family protein -
  BWEI_RS16280 (bwei_3443) nth 3280357..3281004 (-) 648 WP_002030794.1 endonuclease III -
  BWEI_RS16285 (bwei_3444) dnaD 3281020..3281727 (-) 708 WP_033708938.1 DNA replication protein DnaD -

Sequence

Protein


Download         Length: 200 a.a.        Molecular weight: 23347.59 Da        Isoelectric Point: 9.5601

>NTDB_id=131787 BWEI_RS16265 WP_002030800.1 3276404..3277006(+) (recU) [Bacillus mycoides strain WSBC10204]
MTIRYPNGKRYDQASQPHKTPIKKHTYSNRGMSLEEELNDTNQYYLTHNIACVHKKPTPLQIVKVDYPARSAAVVKEAYF
KQPSTTDYNGVYKGKYIDFEAKETKNKTSFPLQNFHLHQIEHMKQVVAHNGIAFVIIKFTLFDEFYLLDAKHIIAFWNRQ
NTGGRKSITKQEIEEHGSLLSCGYHPRIDYIRVLDTVYFS

Nucleotide


Download         Length: 603 bp        

>NTDB_id=131787 BWEI_RS16265 WP_002030800.1 3276404..3277006(+) (recU) [Bacillus mycoides strain WSBC10204]
ATGACCATTCGTTACCCAAATGGAAAAAGGTACGATCAAGCTTCACAACCTCATAAAACACCAATAAAAAAACATACTTA
CAGTAATAGAGGTATGTCCCTTGAAGAGGAATTGAATGACACCAATCAATATTACTTAACCCATAATATTGCATGCGTAC
ATAAAAAACCTACACCTCTTCAAATTGTAAAAGTAGATTACCCCGCTCGAAGTGCTGCAGTGGTAAAAGAAGCGTATTTC
AAACAACCTTCTACAACAGATTACAACGGTGTATACAAAGGGAAATACATCGATTTTGAAGCGAAAGAAACTAAAAATAA
AACCAGTTTTCCACTTCAAAACTTCCACCTTCACCAAATTGAACATATGAAGCAAGTAGTCGCTCATAATGGAATTGCAT
TTGTTATTATTAAATTTACACTTTTTGATGAATTTTATTTATTAGATGCAAAACATATTATTGCATTTTGGAATCGTCAA
AATACTGGTGGACGTAAATCGATTACAAAACAAGAAATAGAAGAGCATGGGTCTTTATTATCATGCGGTTATCACCCTCG
AATTGATTATATCCGCGTACTAGACACGGTTTATTTTTCGTGA

Domains

Predicted by InterproScan.

Recombination protein U (RecU)

(30-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A366GKC2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

62.5

100

0.625

Multiple sequence alignment