Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NL30_RS27055 Genome accession   NZ_CP009744
Coordinates   2541547..2543052 (+) Length   501 a.a.
NCBI ID   WP_046544390.1    Uniprot ID   -
Organism   Burkholderia contaminans strain MS14     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2536547..2548052
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NL30_RS27030 (NL30_27020) kdsD 2537791..2538774 (+) 984 WP_047852234.1 arabinose 5-phosphate isomerase KdsD -
  NL30_RS27035 (NL30_27025) - 2538774..2539310 (+) 537 WP_046544393.1 HAD family hydrolase -
  NL30_RS27040 (NL30_27030) lptC 2539314..2539928 (+) 615 WP_046544392.1 LPS export ABC transporter periplasmic protein LptC -
  NL30_RS27045 (NL30_27035) lptA 2539949..2540614 (+) 666 WP_039346006.1 lipopolysaccharide transport periplasmic protein LptA -
  NL30_RS27050 (NL30_27040) lptB 2540611..2541387 (+) 777 WP_046544391.1 LPS export ABC transporter ATP-binding protein -
  NL30_RS27055 (NL30_27045) rpoN/rpoN1 2541547..2543052 (+) 1506 WP_046544390.1 RNA polymerase factor sigma-54 Machinery gene
  NL30_RS27060 (NL30_27050) raiA 2543211..2543570 (+) 360 WP_006477786.1 ribosome-associated translation inhibitor RaiA -
  NL30_RS27065 (NL30_27055) - 2543739..2544278 (+) 540 WP_046544389.1 PTS sugar transporter subunit IIA -
  NL30_RS27070 (NL30_27060) hprK 2544408..2545376 (+) 969 WP_011353227.1 HPr(Ser) kinase/phosphatase -
  NL30_RS27075 (NL30_27065) rapZ 2545452..2546360 (+) 909 WP_046544388.1 RNase adapter RapZ -
  NL30_RS27080 (NL30_27070) - 2546388..2547023 (+) 636 WP_039346030.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54695.80 Da        Isoelectric Point: 4.8740

>NTDB_id=131761 NL30_RS27055 WP_046544390.1 2541547..2543052(+) (rpoN/rpoN1) [Burkholderia contaminans strain MS14]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPAA
ANGSSSSSDRAERDEPAGADEYDGYGSGDGNDGPQWNLDEFGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=131761 NL30_RS27055 WP_046544390.1 2541547..2543052(+) (rpoN/rpoN1) [Burkholderia contaminans strain MS14]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTGGCGCTGACGCCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGCCCGCTGCGTGTCGCTGCGGACGGATCGCTGATCGCGCAAACGCCTCCCGCGCAGAACACCGAGCCCGCTGCG
GCAAACGGTAGCAGCTCGTCGAGCGATCGTGCCGAGCGCGACGAGCCGGCCGGCGCGGACGAATACGACGGTTACGGGTC
CGGCGACGGCAACGACGGCCCGCAGTGGAACCTCGACGAATTCGGCCGCTCGAGCGGCGCATCCGACGACGACGACCTCC
CGCCGCTGCAGGTGCAGGAAGCGGCGACGTCGCTGCGCGACCACCTGTCCGCGCAGTTGCGCGTCACGCAGGCCGGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGATGAAGTGCT
CGCCGACCTGCCGCCCGAGCTGGAGATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGATCCAG
CCGGGGTCGGTGCCCGTTCGGCATCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTCGATCCGTCCCCGACACGCACGCTC
GCGCTCGAGATCGTGTCGCAGCATCTCGAACTGCTCGCCGCCCGCGACTTCACGCGATTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGTTCGCTGGAGCCGTTCCCTGGCGCCGCATACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGATGGCTCGCGGAGCTCAATCCGGAGGTCGTGCCG
CGCCTGCGGATCAACAACCTTTACGCGAACATCCTGCGCAACAGCCGCGGCGACCCGTCGGCCGGTTCGCTCAAGCAGCA
GCTCCAGGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGTTTCGACACGATCCTGCGTGTCGCGCAGGCGATTGTCG
AGCGTCAAAAGAACTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTTTCGACCGACACCGGTGGCGCCGCTTCGTCGACAGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCTAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAAGGATTCGTGGTCGCGCGC
CGCACGGTTGCGAAGTATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.577

100

0.693

Multiple sequence alignment