Detailed information NTG0131604

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: ADZ28_RS14060
• Genome accession: NZ_CP012127
• Coordinates: 2841005..2841997 (-)
• Length: 330 a.a.
• NCBI ID: WP_000081586.1
• Uniprot ID: Q540P1
• Organism: Escherichia coli strain DH1Ec169
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 2836005..2846997

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ADZ28_RS14040 (ADZ28_14070)	otnK	2836781..2837947 (+)	1167	WP_001393459.1	3-oxo-tetronate kinase	-
ADZ28_RS14045 (ADZ28_14075)	ygbL	2838039..2838677 (+)	639	WP_001278994.1	aldolase	-
ADZ28_RS14050 (ADZ28_14080)	ygbM	2838682..2839458 (+)	777	WP_001136934.1	HPr family phosphocarrier protein	-
ADZ28_RS14055 (ADZ28_14085)	ygbN	2839547..2840911 (+)	1365	WP_000104441.1	GntP family transporter	-
ADZ28_RS14060 (ADZ28_14090)	rpoS	2841005..2841997 (-)	993	WP_000081586.1	RNA polymerase sigma factor RpoS	Regulator
ADZ28_RS14065 (ADZ28_14095)	nlpD	2842060..2843154 (-)	1095	WP_306174120.1	murein hydrolase activator NlpD	-
ADZ28_RS14070 (ADZ28_14100)	pcm	2843339..2843965 (-)	627	WP_000254708.1	protein-L-isoaspartate O-methyltransferase	-
ADZ28_RS14075 (ADZ28_14105)	surE	2843959..2844720 (-)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
ADZ28_RS14080 (ADZ28_14110)	truD	2844701..2845750 (-)	1050	WP_000568970.1	tRNA pseudouridine(13) synthase TruD	-
ADZ28_RS14085 (ADZ28_14115)	ispF	2845747..2846226 (-)	480	WP_001219242.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
ADZ28_RS14090 (ADZ28_14120)	ispD	2846226..2846936 (-)	711	WP_000246138.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37956.89 Da
• Isoelectric Point: 4.5886

>NTDB_id=131604 ADZ28_RS14060 WP_000081586.1 2841005..2841997(-) (rpoS) [Escherichia coli strain DH1Ec169]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVELEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=131604 ADZ28_RS14060 WP_000081586.1 2841005..2841997(-) (rpoS) [Escherichia coli strain DH1Ec169]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAACTGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 region 3 (Sigma70_r3) (174-248)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

3D structure

• AlphaFold DB: Q540P1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715