Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BF36_RS05660 Genome accession   NZ_CP009720
Coordinates   917687..918121 (+) Length   144 a.a.
NCBI ID   WP_000811492.1    Uniprot ID   A0A0G8EA73
Organism   Bacillus thuringiensis strain HD682     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 912687..923121
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BF36_RS05640 (BF36_1015) - 913576..914739 (-) 1164 WP_000434592.1 aminotransferase A -
  BF36_RS05645 (BF36_1016) - 914862..915062 (-) 201 WP_000929284.1 hypothetical protein -
  BF36_RS05650 (BF36_1017) kinB 915373..916647 (+) 1275 WP_000420390.1 sporulation sensor histidine kinase KinB -
  BF36_RS05655 (BF36_1018) - 916701..917450 (-) 750 WP_000388464.1 DUF3967 domain-containing protein -
  BF36_RS05660 (BF36_1019) nucA/comI 917687..918121 (+) 435 WP_000811492.1 DNA-entry nuclease Machinery gene
  BF36_RS05665 (BF36_1020) comJ 918158..918547 (+) 390 WP_000424033.1 competence protein ComJ -
  BF36_RS05670 (BF36_1021) metE 918584..920872 (-) 2289 WP_001007635.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  BF36_RS05680 (BF36_1022) - 921797..922354 (-) 558 WP_042510888.1 PadR family transcriptional regulator -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16012.35 Da        Isoelectric Point: 8.4331

>NTDB_id=131569 BF36_RS05660 WP_000811492.1 917687..918121(+) (nucA/comI) [Bacillus thuringiensis strain HD682]
MKQLKGIIISIIAILSILVAVYEVLVPEETSTKKTNAYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=131569 BF36_RS05660 WP_000811492.1 917687..918121(+) (nucA/comI) [Bacillus thuringiensis strain HD682]
ATGAAGCAATTAAAAGGTATTATAATTTCAATTATTGCAATTCTTTCTATTTTAGTAGCGGTTTATGAAGTACTTGTTCC
AGAGGAAACAAGTACAAAAAAAACAAATGCGTATGATCAAGTTCTAGAATTTCCGAAAGAGAGATATCCAGAAACAGGGA
AACATATTACGGATGCGATAAAGGAAGGACATTCAGAAGTTTGTACAATCGACCGTGGTGGCGCTGCGGATAGAAGAAAG
TTATCGTTAGCTCCATACCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGAGCACATATCGAATATATAAGTCCAGCAGATAACCGCGGAGCAGGGTCTTGGGTAGGAAATAAGTTAGATAAATACC
CAGATGGTACGCGTGTGAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0G8EA73

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

70.796

78.472

0.556

Multiple sequence alignment