Detailed information NTG0131444

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: ADS71_RS14060
• Genome accession: NZ_CP012125
• Coordinates: 2844469..2845461 (-)
• Length: 330 a.a.
• NCBI ID: WP_000081586.1
• Uniprot ID: Q540P1
• Organism: Escherichia coli strain DH1Ec095
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 2839469..2850461

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ADS71_RS14040 (ADS71_14070)	otnK	2840245..2841411 (+)	1167	WP_001393459.1	3-oxo-tetronate kinase	-
ADS71_RS14045 (ADS71_14075)	ygbL	2841503..2842141 (+)	639	WP_001278994.1	aldolase	-
ADS71_RS14050 (ADS71_14080)	ygbM	2842146..2842922 (+)	777	WP_001136934.1	HPr family phosphocarrier protein	-
ADS71_RS14055 (ADS71_14085)	ygbN	2843011..2844375 (+)	1365	WP_000104441.1	GntP family transporter	-
ADS71_RS14060 (ADS71_14090)	rpoS	2844469..2845461 (-)	993	WP_000081586.1	RNA polymerase sigma factor RpoS	Regulator
ADS71_RS14065 (ADS71_14095)	nlpD	2845524..2846618 (-)	1095	WP_306174120.1	murein hydrolase activator NlpD	-
ADS71_RS14070 (ADS71_14100)	pcm	2846803..2847429 (-)	627	WP_000254708.1	protein-L-isoaspartate O-methyltransferase	-
ADS71_RS14075 (ADS71_14105)	surE	2847423..2848184 (-)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
ADS71_RS14080 (ADS71_14110)	truD	2848165..2849214 (-)	1050	WP_000568970.1	tRNA pseudouridine(13) synthase TruD	-
ADS71_RS14085 (ADS71_14115)	ispF	2849211..2849690 (-)	480	WP_001219242.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
ADS71_RS14090 (ADS71_14120)	ispD	2849690..2850400 (-)	711	WP_000246138.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37956.89 Da
• Isoelectric Point: 4.5886

>NTDB_id=131444 ADS71_RS14060 WP_000081586.1 2844469..2845461(-) (rpoS) [Escherichia coli strain DH1Ec095]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVELEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=131444 ADS71_RS14060 WP_000081586.1 2844469..2845461(-) (rpoS) [Escherichia coli strain DH1Ec095]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAACTGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 region 3 (Sigma70_r3) (174-248)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

3D structure

• AlphaFold DB: Q540P1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715