Detailed information NTG0131087

Bioinformatically predicted

Overview

• Name: comEC
• Type: Machinery gene
• Locus tag: NG74_RS12040
• Genome accession: NZ_CP009679
• Coordinates: 2537776..2540127 (-)
• Length: 783 a.a.
• NCBI ID: WP_022552933.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain JS25R
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2532776..2545127

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NG74_RS12010 (NG74_02488)	-	2533142..2533480 (-)	339	WP_007408270.1	YqxA family protein	-
NG74_RS12015 (NG74_02489)	spoIIP	2533497..2534690 (-)	1194	WP_007612673.1	stage II sporulation protein P	-
NG74_RS12020 (NG74_02490)	gpr	2534758..2535864 (-)	1107	WP_022552935.1	GPR endopeptidase	-
NG74_RS12025 (NG74_02491)	rpsT	2536067..2536333 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
NG74_RS12030 (NG74_02492)	holA	2536350..2537391 (-)	1042	Protein_2382	DNA polymerase III subunit delta	-
NG74_RS20145	-	2537431..2537582 (-)	152	Protein_2383	hypothetical protein	-
NG74_RS19750	-	2537623..2537757 (+)	135	WP_003152870.1	YqzM family protein	-
NG74_RS12040 (NG74_02493)	comEC	2537776..2540127 (-)	2352	WP_022552933.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
NG74_RS12045 (NG74_02494)	-	2540128..2540697 (-)	570	WP_003152868.1	ComE operon protein 2	-
NG74_RS12050 (NG74_02495)	comEA	2540764..2541378 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
NG74_RS12055 (NG74_02496)	comER	2541437..2542258 (+)	822	WP_014418420.1	late competence protein ComER	-
NG74_RS12060 (NG74_02497)	-	2542327..2543064 (-)	738	WP_014418421.1	class I SAM-dependent DNA methyltransferase	-
NG74_RS12065 (NG74_02498)	rsfS	2543061..2543417 (-)	357	WP_007408260.1	ribosome silencing factor	-
NG74_RS12070 (NG74_02499)	yqeK	2543435..2543995 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
NG74_RS12075 (NG74_02500)	-	2543985..2544554 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
NG74_RS12080 (NG74_02501)	yhbY	2544565..2544855 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-

Sequence

Protein

• Length: 783 a.a.
• Molecular weight: 86713.55 Da
• Isoelectric Point: 8.7941

>NTDB_id=131087 NG74_RS12040 WP_022552933.1 2537776..2540127(-) (comEC) [Bacillus velezensis strain JS25R]
MKYKYLLLPLAAVSATAGIAAAHVFWVLLLFLLYLFFIIVKTKQHAPVIVCLVSFCLYFFLYTVCDAANVTRYQAGSYTE
QVVITNIPKVDGAKMSAVIRTHDKEKWAASYKIRSLEEKRLIEQLEPGMRCTFTGSLEQPAHATVPGGFDYNEYLYSQQI
HWLFSVTSIQQCEKSKQPLFKLLNIRKNLISIIRNHVPESSAGIVEALTLGERFSIEDDILSAYQNLGVVHLMAISGMHV
GLITAGLFYALIRIGLTREKAGILLLLFLPVYTLLSGAAPSVLRASLMLGFYIAGTLVKRGIHSSAALSLSYLLLLLFNP
YFLWQAGFQLSFAVSASLILSSSILKKAGKSRLAGLAMASFIAELSSLPFLLYHFQQISLVSFPMNMVMVPFYTLFVIPV
SVIGFLLLLLSGQMGECLFDMFDLVMKPVHDFITYAASVDLFTMIVSKPDFLSLLLLAVSVFTLFAALEKGGFLKLRKSV
LFFCAVLAYLICRPYFSPWGEADMLDIGQGDSLFISAPHRKGTVMVDTGGVIAYPGESWKEKRHPYSIGEKVLIPFLNGK
GVKKLDALILTHADQDHIGEAGVLIKNHRVKRLIVPVGFVKEPKDQNILNMAKENNIPVAEAKRGDTITAGDLQFQVLSP
ESSDGRSKNDSSLVLWTVLSGVSWLLTGDLESDGETEVLKTYPNLKADILKAGHHGSKSSTSEAFLKQLQPEAALISAGK
ENRYHHPHEEVLDRLKAYSVNVLRTDISGTIQYRFKKGAGTFSVFPPYDIEEIRAQEVKKTAD

Nucleotide

• Length: 2352 bp

>NTDB_id=131087 NG74_RS12040 WP_022552933.1 2537776..2540127(-) (comEC) [Bacillus velezensis strain JS25R]
ATGAAATATAAATACCTTCTTCTGCCTCTGGCGGCGGTTTCTGCAACTGCGGGAATTGCCGCCGCTCATGTCTTCTGGGT
TCTGCTCCTTTTTCTTCTGTATCTTTTCTTTATTATTGTAAAAACAAAGCAGCATGCTCCGGTTATTGTCTGCCTCGTTT
CTTTTTGTCTTTATTTCTTTCTTTATACGGTTTGTGACGCTGCGAATGTAACGCGGTATCAGGCCGGCAGTTATACTGAA
CAGGTCGTCATCACTAACATTCCGAAGGTTGACGGAGCGAAAATGTCAGCCGTTATCCGTACACATGACAAGGAAAAATG
GGCGGCTTCGTACAAAATCCGGTCTCTTGAGGAAAAGAGACTCATTGAACAGCTTGAACCGGGGATGCGCTGCACGTTTA
CAGGTTCTCTGGAGCAGCCTGCACATGCGACGGTTCCCGGAGGTTTTGATTATAATGAATATCTTTACTCTCAGCAGATT
CACTGGTTATTTTCCGTGACTTCCATTCAGCAGTGTGAAAAATCCAAACAGCCGCTGTTTAAACTGCTGAACATCAGAAA
AAATTTGATTTCGATCATTCGGAATCACGTGCCTGAATCTTCCGCCGGAATTGTTGAAGCGCTGACCTTAGGTGAAAGAT
TTTCTATAGAGGACGATATACTGAGTGCATATCAAAATTTGGGAGTCGTTCATTTAATGGCGATTTCCGGAATGCATGTC
GGTCTTATTACGGCGGGATTATTTTATGCTCTGATCAGAATCGGGCTGACAAGAGAAAAAGCGGGAATTTTGCTACTGTT
GTTTTTGCCGGTGTATACGCTGCTGAGCGGTGCCGCCCCGTCCGTATTGCGCGCATCCCTCATGCTGGGATTTTATATCG
CCGGAACTCTTGTTAAACGCGGCATTCATTCCTCCGCTGCATTGTCCCTGTCTTATCTGCTGCTCCTGCTGTTTAATCCT
TACTTCCTTTGGCAGGCGGGCTTCCAGCTTTCCTTTGCGGTAAGCGCCTCTTTAATTCTGTCATCCTCCATTTTAAAGAA
AGCAGGGAAAAGCAGACTTGCCGGGCTTGCGATGGCTTCATTCATTGCGGAGCTCAGCTCACTTCCGTTTCTTCTCTATC
ATTTTCAGCAGATTTCACTTGTCAGTTTTCCGATGAATATGGTGATGGTGCCATTTTATACGTTATTTGTCATTCCGGTT
TCTGTCATTGGTTTCCTTCTTCTTTTACTTTCAGGGCAGATGGGAGAATGTTTGTTTGATATGTTCGACCTTGTGATGAA
GCCTGTACATGATTTCATTACATATGCGGCATCCGTTGATTTATTTACTATGATTGTGTCAAAGCCTGACTTTCTTTCCC
TTCTTCTGCTTGCGGTTTCCGTTTTTACTCTTTTTGCGGCTTTGGAAAAGGGAGGTTTTTTAAAACTCAGGAAATCGGTT
CTTTTTTTCTGCGCGGTTTTGGCTTATTTAATATGCCGTCCGTATTTCAGTCCATGGGGAGAAGCGGATATGCTTGATAT
CGGGCAGGGAGACTCACTGTTTATAAGCGCGCCGCACCGCAAAGGGACCGTAATGGTTGATACAGGGGGAGTGATTGCTT
ATCCCGGAGAATCATGGAAAGAAAAACGCCACCCGTATTCTATCGGCGAGAAGGTTTTGATTCCGTTTTTAAACGGAAAA
GGGGTGAAAAAGCTGGATGCGCTGATTTTAACCCATGCGGATCAAGATCACATCGGAGAAGCCGGAGTGTTAATCAAAAA
TCATAGAGTCAAACGGTTAATTGTCCCCGTGGGATTCGTAAAAGAACCGAAGGATCAGAACATATTAAATATGGCGAAAG
AAAACAACATTCCCGTTGCCGAAGCAAAGCGGGGCGACACCATTACAGCCGGTGATCTTCAGTTTCAGGTGCTGTCTCCG
GAGTCGTCTGACGGAAGGAGTAAAAATGATTCGTCACTGGTGCTTTGGACGGTTTTAAGCGGAGTGAGCTGGCTTTTGAC
GGGAGATTTAGAATCGGACGGCGAAACGGAAGTGCTGAAAACGTATCCGAATCTGAAGGCTGATATATTGAAGGCGGGTC
ATCACGGCAGCAAAAGCTCAACGAGTGAAGCCTTTTTGAAACAGCTTCAGCCGGAAGCGGCGCTGATTTCAGCAGGAAAA
GAGAATCGATACCATCATCCGCATGAAGAAGTGCTGGATCGTTTGAAGGCGTACTCTGTCAATGTGCTTCGCACCGATAT
CAGCGGAACGATTCAATACAGATTTAAAAAAGGCGCCGGAACGTTTTCCGTCTTCCCTCCATATGATATAGAAGAAATCA
GGGCGCAAGAAGTAAAAAAGACTGCCGATTGA

Domains

Predicted by InterproScan.

• Metallo-beta-lactamase superfamily (Lactamase_B) (508-719)

• Domain of unknown function (DUF4131) (DUF4131) (26-171)

• Competence protein (Competence) (208-469)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC	Bacillus subtilis subsp. subtilis str. 168	56.921	98.723	0.562