Detailed information    

insolico Bioinformatically predicted

Overview

Name   recA   Type   Machinery gene
Locus tag   NG74_RS08405 Genome accession   NZ_CP009679
Coordinates   1695557..1696600 (+) Length   347 a.a.
NCBI ID   WP_003154145.1    Uniprot ID   Q659J5
Organism   Bacillus velezensis strain JS25R     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1690557..1701600
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NG74_RS08375 (NG74_01733) ymfI 1690565..1691293 (+) 729 WP_014417800.1 elongation factor P 5-aminopentanone reductase -
  NG74_RS08380 (NG74_01734) - 1691369..1691626 (+) 258 WP_003154150.1 DUF3243 domain-containing protein -
  NG74_RS08385 (NG74_01735) - 1691754..1692545 (+) 792 WP_003154149.1 DUF3388 domain-containing protein -
  NG74_RS08390 (NG74_01736) - 1692564..1693463 (+) 900 WP_014417801.1 helix-turn-helix domain-containing protein -
  NG74_RS08395 (NG74_01737) pgsA 1693537..1694118 (+) 582 WP_007409821.1 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase -
  NG74_RS08400 (NG74_01738) cinA 1694136..1695386 (+) 1251 WP_021493669.1 competence/damage-inducible protein A Machinery gene
  NG74_RS08405 (NG74_01739) recA 1695557..1696600 (+) 1044 WP_003154145.1 recombinase RecA Machinery gene
  NG74_RS08410 (NG74_01740) - 1696767..1697948 (+) 1182 WP_017417799.1 serine hydrolase domain-containing protein -
  NG74_RS08415 (NG74_01741) rny 1698241..1699800 (+) 1560 WP_003154140.1 ribonuclease Y -
  NG74_RS08420 (NG74_01742) - 1699861..1700655 (+) 795 WP_038461458.1 TIGR00282 family metallophosphoesterase -
  NG74_RS08425 (NG74_01743) spoVS 1700855..1701115 (+) 261 WP_003154135.1 stage V sporulation protein SpoVS -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 37957.17 Da        Isoelectric Point: 4.6955

>NTDB_id=131073 NG74_RS08405 WP_003154145.1 1695557..1696600(+) (recA) [Bacillus velezensis strain JS25R]
MSDRQAALDMALKQIEKQFGKGSIMKLGEKTDTRISTVPSGSLALDTALGIGGYPRGRIIEVYGPESSGKTTVALHAIAE
VQEKGGQAAFIDAEHALDPVYAQKLGVNIEELLLSQPDTGEQALEIAEALVRSGAVDIVVVDSVAALVPKAEIEGDMGDS
HVGLQARLMSQALRKLSGAINKSKTIAIFINQIREKVGVMFGNPETTPGGRALKFYSSVRLEVRRAEQLKQGNDVMGNKT
RIKVVKNKVAPPFRTAEVDIMYGEGISKEGEIIDLGTELDIVQKSGSWYSYEEERLGQGRENAKQFLKENKDIMLMIQEQ
IREYYGLDNNGVTDKAEEVQEEMELEE

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=131073 NG74_RS08405 WP_003154145.1 1695557..1696600(+) (recA) [Bacillus velezensis strain JS25R]
ATGAGTGATCGTCAGGCAGCCTTAGATATGGCTCTTAAGCAAATAGAAAAACAATTCGGCAAAGGTTCCATCATGAAGCT
CGGAGAAAAAACGGATACAAGAATTTCAACGGTGCCAAGCGGTTCCCTTGCACTTGATACCGCTCTCGGAATAGGCGGAT
ACCCGCGCGGACGGATTATTGAAGTATACGGACCTGAAAGCTCAGGTAAAACGACTGTAGCGCTTCACGCAATCGCTGAG
GTTCAGGAAAAAGGCGGACAGGCAGCATTTATTGATGCTGAGCATGCTCTTGATCCTGTTTACGCGCAAAAGCTCGGTGT
CAATATCGAAGAGCTTCTGCTTTCTCAGCCGGATACGGGAGAGCAGGCGCTAGAGATTGCTGAAGCGCTGGTGCGAAGCG
GAGCTGTTGATATCGTAGTCGTTGACTCTGTTGCGGCGCTTGTTCCAAAAGCTGAAATTGAAGGTGACATGGGTGATTCA
CACGTCGGTTTACAGGCGCGTCTCATGTCTCAGGCGCTCCGTAAGCTTTCCGGCGCCATCAATAAATCTAAAACAATCGC
AATCTTTATTAACCAGATTCGTGAAAAAGTCGGCGTTATGTTCGGAAATCCGGAGACGACACCGGGGGGCCGCGCGCTGA
AATTCTATTCTTCCGTGCGCCTTGAAGTGCGCCGTGCCGAGCAATTAAAGCAGGGCAACGACGTCATGGGGAATAAAACG
AGAATTAAAGTCGTAAAAAACAAAGTCGCTCCTCCGTTCCGTACGGCTGAAGTGGACATTATGTACGGTGAAGGAATCTC
CAAAGAAGGGGAAATCATCGACCTTGGAACTGAACTGGATATCGTGCAAAAAAGCGGTTCATGGTATTCTTATGAAGAAG
AACGCCTCGGCCAGGGCCGTGAAAACGCCAAGCAGTTCTTAAAAGAAAATAAAGACATCATGCTGATGATTCAGGAGCAA
ATCCGTGAATATTACGGTTTGGACAATAACGGTGTAACGGACAAAGCGGAAGAAGTTCAGGAAGAGATGGAATTAGAAGA
ATAA

Domains

Predicted by InterproScan.

recA bacterial DNA recombination protein (RecA)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q659J5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recA Bacillus subtilis subsp. subtilis str. 168

96.839

100

0.971
  recA Latilactobacillus sakei subsp. sakei 23K

74.924

94.236

0.706
  recA Streptococcus mutans UA159

66.092

100

0.663
  recA Streptococcus pneumoniae D39

68.485

95.101

0.651
  recA Streptococcus mitis NCTC 12261

68.485

95.101

0.651
  recA Streptococcus pneumoniae Rx1

68.485

95.101

0.651
  recA Streptococcus pneumoniae R6

68.485

95.101

0.651
  recA Streptococcus pneumoniae TIGR4

68.485

95.101

0.651
  recA Streptococcus mitis SK321

68.182

95.101

0.648
  recA Streptococcus pyogenes NZ131

67.378

94.524

0.637
  recA Helicobacter pylori 26695

63.265

98.847

0.625
  recA Helicobacter pylori strain NCTC11637

63.265

98.847

0.625
  recA Lactococcus lactis subsp. cremoris KW2

65.455

95.101

0.622
  recA Neisseria gonorrhoeae strain FA1090

65.443

94.236

0.617
  recA Neisseria gonorrhoeae MS11

65.443

94.236

0.617
  recA Neisseria gonorrhoeae MS11

65.443

94.236

0.617
  recA Ralstonia pseudosolanacearum GMI1000

64.329

94.524

0.608
  recA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

63.609

94.236

0.599
  recA Riemerella anatipestifer ATCC 11845 = DSM 15868

63.609

94.236

0.599
  recA Glaesserella parasuis strain SC1401

60.29

99.424

0.599
  recA Acinetobacter baylyi ADP1

59.71

99.424

0.594
  recA Vibrio cholerae strain A1552

64.174

92.507

0.594
  recA Vibrio cholerae O1 biovar El Tor strain E7946

64.174

92.507

0.594
  recA Pseudomonas stutzeri DSM 10701

63.125

92.219

0.582
  recA Acinetobacter baumannii D1279779

62.305

92.507

0.576
  recA Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

60.625

92.219

0.559

Multiple sequence alignment