Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NG74_RS01665 Genome accession   NZ_CP009679
Coordinates   319664..320101 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain JS25R     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 314664..325101
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NG74_RS01640 (NG74_00338) - 315684..316820 (+) 1137 WP_007609290.1 zinc-dependent alcohol dehydrogenase -
  NG74_RS01645 (NG74_00339) - 316835..317269 (+) 435 WP_014416871.1 RDD family protein -
  NG74_RS01650 (NG74_00340) - 317386..317664 (+) 279 WP_263612330.1 YckD family protein -
  NG74_RS01655 (NG74_00341) - 317768..319204 (+) 1437 WP_022552568.1 glycoside hydrolase family 1 protein -
  NG74_RS01660 (NG74_00342) nin/comJ 319245..319643 (-) 399 WP_022552569.1 competence protein ComJ Regulator
  NG74_RS01665 (NG74_00343) nucA/comI 319664..320101 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  NG74_RS01670 (NG74_00344) hxlB 320432..320989 (-) 558 WP_014416874.1 6-phospho-3-hexuloisomerase -
  NG74_RS01675 (NG74_00345) hxlA 320986..321621 (-) 636 WP_015239130.1 3-hexulose-6-phosphate synthase -
  NG74_RS01680 (NG74_00346) - 321853..322215 (+) 363 WP_007409352.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=131046 NG74_RS01665 WP_003156588.1 319664..320101(-) (nucA/comI) [Bacillus velezensis strain JS25R]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=131046 NG74_RS01665 WP_003156588.1 319664..320101(-) (nucA/comI) [Bacillus velezensis strain JS25R]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCCGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment