Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   BG07_RS08200 Genome accession   NZ_CP009651
Coordinates   1177421..1180933 (-) Length   1170 a.a.
NCBI ID   WP_040119298.1    Uniprot ID   -
Organism   Bacillus pseudomycoides strain BTZ     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1172421..1185933
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BG07_RS08180 (BG07_1187) - 1172595..1172981 (+) 387 WP_006093913.1 spore germination protein GerPE -
  BG07_RS08185 (BG07_1188) gerPF 1173027..1173242 (+) 216 WP_003195867.1 spore germination protein GerPF -
  BG07_RS08190 (BG07_1189) - 1173328..1173621 (-) 294 WP_006093912.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  BG07_RS08195 (BG07_1190) addA 1173699..1177424 (-) 3726 WP_040119299.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  BG07_RS08200 (BG07_1191) addB 1177421..1180933 (-) 3513 WP_040119298.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  BG07_RS08205 (BG07_1192) lepB 1181059..1181622 (-) 564 WP_018783054.1 signal peptidase I -
  BG07_RS08210 (BG07_1193) - 1181681..1182274 (-) 594 WP_006093908.1 TVP38/TMEM64 family protein -
  BG07_RS08215 (BG07_1194) - 1182674..1184461 (-) 1788 WP_040119297.1 PAS domain-containing sensor histidine kinase -
  BG07_RS33725 - 1184533..1184798 (-) 266 Protein_1212 DDE-type integrase/transposase/recombinase -

Sequence

Protein


Download         Length: 1170 a.a.        Molecular weight: 133996.87 Da        Isoelectric Point: 4.9498

>NTDB_id=130975 BG07_RS08200 WP_040119298.1 1177421..1180933(-) (addB) [Bacillus pseudomycoides strain BTZ]
MSLRFVIGRAGSGKSTFCLHEVQEELGQRPRGETILYLVPEQMTFQTQQALIGNEGVSASIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVEARKEGLYVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSGDEQKLLA
NKMYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPDSDYVKGAEIYIDGFHSFSPGELEIIRQLMICGARITITLTVDE
KTLSQPTSELDLFYQTVLTYEKVKQLAREEKIAVESTISMSEQPRFLSPALAHLEAEFESRPSKKFQSEPDITLYTAANL
RAEVEGVAREIRRLVAEEEYRYRDITVLLRNGESYYDVMKTLFTDYDIPHFIDEKRPMSHHPLIECIRSALEVISGNWRY
DAIFRCIKTELLYPLDVRKESMREEMDEFENYCLAYGVQGKRWTADEPWTYRRYRSLDGVNGMQTDSEREMEEKINCLRD
VVRTPIIRLQKRLKRASSVVQMCEAVYLFLEELDVPKKLEDLRMRAEAEGNFLFASDHDQVWEEVMNLLDTFVEMLGEEK
MSRSMFIDVMTTGLEALQFANIPPSLDQVLIANIDRSRLSDIRAAFVIGANEGIIPAAPSDEGMLSDEEREFLVTAGVEL
APTTRQTLLEEQFVIYQTVTRASEKLYISCPLADEEGKTLLSSSFIKKVKRMFPSVKEVFVTNDVNDLSRQVQISYVATP
EVTLSYVTQQLQNWKRYGFEGNLDFWWDVYNFYVTSPEWKQKSSRVLSSLFYRNRAQKLSSVVSRDLYGDKIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKKIADKLLRENRTWADLSIKECEHLSAVVIEEIAPLLQRQIL
LSSNRNYYLKQKLQQIIFRTSLILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TFLRIIDYKSSTKALDLTEVYYGLALQMLTYLDVVISNANTWMKKDGTASPAGVLYFHIHNPIVEMKGDASEEEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFTTLQQYVHHTFENIGKDITEGVIDIAP
YKMGNKAACTFCNFRSVCQFDESLEDNQFRTLKDMKDSEAMEKMREEVGE

Nucleotide


Download         Length: 3513 bp        

>NTDB_id=130975 BG07_RS08200 WP_040119298.1 1177421..1180933(-) (addB) [Bacillus pseudomycoides strain BTZ]
ATGTCGCTTCGATTTGTGATTGGACGAGCTGGAAGTGGAAAAAGTACATTTTGTTTACATGAAGTGCAAGAAGAATTAGG
GCAGCGTCCAAGAGGCGAAACAATTTTATATCTTGTACCGGAACAAATGACGTTCCAAACGCAGCAAGCTTTAATAGGTA
ATGAAGGAGTTAGCGCCTCCATCAGGGCGCAAGTCTTTAGTTTTTCAAGGTTAGCGTGGAAGGTCTTGCAAGAAGTTGGT
GGTGCAAGCCGCCTTCATATTGATGAGGCTGGCGTGCATATGTTACTACGTAAAATTGTAGAAGCGCGTAAAGAAGGATT
GTATGTGTTTCAAAAAGCAGCAGAACAAAATGGCTTTTTTGAGCATTTAGGCAGCATGATTGCCGAGTTTAAACGTTATA
ATGTAACGCCATCGAATGTATATGAGATGTGGCAACAGTTAGATGCTCACAGTAGCGGTGATGAACAAAAATTATTAGCA
AATAAAATGTACGACCTTCAGCTTTTATATGATGATTTTGAACGGGCTTTAATTGGTAAATATTTAGATTCAGAAGATTA
TTTACAGTTGCTTGTAGAAAAGCTTCCGGATTCTGATTATGTAAAAGGCGCAGAAATTTATATAGACGGATTTCATTCAT
TTTCACCTGGGGAATTAGAGATTATTAGACAGCTCATGATTTGCGGAGCAAGAATAACAATTACGTTAACAGTAGATGAA
AAAACATTATCGCAGCCAACAAGTGAACTAGATTTGTTTTATCAAACAGTACTTACGTATGAAAAGGTAAAACAACTGGC
ACGTGAGGAGAAAATCGCAGTTGAAAGTACAATTTCAATGAGCGAACAGCCACGTTTTCTTTCGCCAGCATTGGCACATC
TAGAAGCTGAGTTTGAATCTCGTCCGTCTAAGAAGTTCCAAAGTGAACCAGACATTACATTATATACAGCGGCAAACTTG
CGAGCGGAAGTTGAAGGTGTAGCTAGGGAAATTCGCAGACTTGTTGCGGAAGAGGAGTATCGCTACAGAGATATTACGGT
TCTTCTTCGTAATGGAGAAAGCTATTATGATGTAATGAAAACATTGTTTACTGATTATGATATTCCGCACTTTATCGATG
AAAAGCGCCCAATGTCACATCATCCATTGATTGAATGCATTCGTTCTGCACTAGAAGTGATTAGTGGGAATTGGCGCTAT
GATGCTATTTTTCGCTGTATAAAAACGGAATTATTATATCCGTTGGATGTGAGAAAAGAATCAATGCGCGAAGAAATGGA
TGAGTTTGAAAATTACTGTTTAGCATATGGCGTACAAGGAAAGAGATGGACGGCAGATGAGCCTTGGACGTATCGTCGTT
ATCGTTCGCTAGATGGAGTAAACGGAATGCAAACAGATAGCGAACGTGAAATGGAAGAGAAAATAAACTGCCTTCGTGAT
GTGGTAAGGACACCAATTATTCGTTTGCAGAAGCGTTTAAAACGTGCAAGTTCAGTTGTACAAATGTGTGAAGCGGTTTA
TTTGTTTTTAGAAGAATTAGATGTTCCTAAAAAACTGGAAGATTTGCGTATGCGTGCTGAGGCAGAAGGGAATTTTCTAT
TTGCAAGTGACCATGATCAAGTTTGGGAAGAAGTAATGAATCTTCTTGATACATTTGTAGAAATGCTTGGTGAGGAAAAG
ATGTCTCGTTCAATGTTTATCGATGTAATGACAACGGGACTTGAGGCACTTCAATTTGCAAATATCCCGCCTTCTTTAGA
TCAAGTGCTCATTGCGAACATTGATCGTTCAAGGTTATCGGATATTCGCGCGGCTTTTGTGATTGGTGCGAATGAAGGAA
TCATTCCGGCAGCCCCTTCAGATGAAGGGATGTTATCGGATGAAGAAAGGGAATTTCTCGTTACGGCTGGTGTAGAATTA
GCACCAACAACAAGGCAGACGTTATTAGAAGAGCAATTTGTTATTTATCAAACAGTAACTCGTGCATCTGAGAAGTTATA
TATTTCTTGCCCCCTTGCGGATGAAGAAGGAAAGACGCTACTTTCATCTAGCTTTATTAAGAAAGTGAAAAGAATGTTTC
CTTCTGTTAAAGAAGTATTTGTAACGAATGATGTGAATGATTTATCTCGTCAGGTACAAATTTCGTATGTAGCAACGCCA
GAGGTTACCTTGTCATATGTAACGCAGCAATTACAAAATTGGAAACGATATGGTTTTGAAGGGAACCTTGATTTTTGGTG
GGATGTTTACAACTTCTATGTTACATCGCCCGAGTGGAAGCAAAAAAGTAGTCGTGTACTATCTAGTTTATTTTATCGAA
ACCGTGCGCAGAAGCTTAGCTCAGTTGTAAGTAGAGATCTATACGGAGATAAAATTAAAGGAAGCGTCTCTCGCATGGAA
TTATTTAATCGATGCGCATATGCTCATTTTGCGCAGCATGGATTATCGCTGAGAGAGCGTGATATTTTCAAGTTAGATGC
ACCGGATATCGGCGAATTATTCCATGCAGCATTAAAGAAAATTGCAGACAAGTTACTTAGGGAAAATCGTACTTGGGCTG
ATTTATCGATAAAAGAGTGTGAGCATCTTTCTGCCGTAGTAATTGAAGAAATCGCACCGTTATTACAAAGACAAATTTTA
TTAAGTTCTAATCGGAATTATTATTTGAAACAAAAGTTACAGCAAATCATTTTCCGTACGTCACTTATCCTTCGTGAACA
TGCGAAGTCCAGTGGATTTGTGCCAGTTGATTTAGAGGTTCCATTTGGTATGGGCGGGACAGGCTCACTTCCGCCGATGG
AGTTTTCGTTGCCAAACGGTGTGAAAATGGAAGTTGTTGGCCGGATTGACCGTGTAGATAAAGCGGAAGACGAAAATGGT
ACCTTCCTTCGCATTATTGATTATAAATCGAGCACAAAAGCTTTAGATTTAACAGAAGTATATTATGGGTTAGCACTTCA
AATGTTAACGTATTTAGACGTTGTTATTTCAAATGCAAATACGTGGATGAAAAAAGATGGAACGGCATCACCTGCTGGCG
TTTTATATTTCCATATTCATAACCCGATTGTGGAGATGAAAGGCGACGCTTCGGAAGAAGAGATTGAAAAGGAAATTTTA
AAGAAATTTAAAATGAAAGGTCTCGTACTAGGAGATGCGGATGTTGTTCGATTGATGGATAACAAGCTTTCAACAGGAAG
TTCAGACATTATTTCTGCAGGTCTGAAAAAAGATGGTAGCTTTAGTGCGCGTTCTAGTATTGCAAGTGAGCAAGAATTTA
CAACCTTACAGCAATATGTACATCATACATTTGAAAACATCGGGAAAGACATTACAGAAGGTGTTATTGATATCGCACCG
TATAAAATGGGGAACAAAGCAGCTTGTACATTCTGTAATTTCCGTTCTGTTTGTCAGTTTGATGAATCACTTGAGGATAA
TCAGTTCCGCACGTTAAAAGATATGAAAGATAGTGAAGCGATGGAGAAAATGAGAGAGGAGGTTGGCGAATGA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)

UvrD-like helicase C-terminal domain (UvrD_C)

(306-669)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

48.547

100

0.485

Multiple sequence alignment