Detailed information NTG0130903

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: AK40_RS22995
• Genome accession: NZ_CP009641
• Coordinates: 3674061..3675410 (-)
• Length: 449 a.a.
• NCBI ID: WP_000225366.1
• Uniprot ID: A0A158RMK4
• Organism: Bacillus cereus 03BB108
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3669061..3680410

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AK40_RS22975 (AK40_3818)	secA	3669383..3671890 (-)	2508	WP_000579383.1	preprotein translocase subunit SecA	-
AK40_RS22980 (AK40_3819)	hpf	3672167..3672709 (-)	543	WP_000671189.1	ribosome hibernation-promoting factor, HPF/YfiA family	-
AK40_RS22985 (AK40_3820)	cspC	3673033..3673230 (-)	198	WP_001990088.1	cold shock protein CspC	-
AK40_RS22990 (AK40_3821)	comFC	3673357..3674061 (-)	705	WP_003162242.1	comF operon protein ComFC	-
AK40_RS22995 (AK40_3822)	comFA	3674061..3675410 (-)	1350	WP_000225366.1	ATP-dependent helicase ComFA	Machinery gene
AK40_RS23000 (AK40_3823)	-	3675538..3676848 (-)	1311	WP_000734330.1	NlpC/P60 family protein	-
AK40_RS23005 (AK40_3824)	-	3677102..3677416 (-)	315	WP_000400857.1	winged helix-turn-helix transcriptional regulator	-
AK40_RS23015 (AK40_3825)	-	3677590..3678432 (-)	843	WP_000844766.1	DegV family protein	-
AK40_RS23020 (AK40_3826)	-	3678672..3679307 (+)	636	WP_000926685.1	YigZ family protein	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 51428.10 Da
• Isoelectric Point: 9.6542

>NTDB_id=130903 AK40_RS22995 WP_000225366.1 3674061..3675410(-) (comFA) [Bacillus cereus 03BB108]
MVAGKQLLLEELSSDLRRELSDLKKKGEIICVQGVIKKASKYICQRCGNIEQRLFASFLCKRCSKVCTYCRKCITMGRVS
ECAVLVRGIHERKGERELHSLQWKGTLSTGQELAAQGVIEAIKQKESFFIWAVCGAGKTEMLFYGIEEALQKEERVCIAT
PRTDVVLELVPRLQEVFPSINVAALYGGSVDREKDAALVVATTHQLLRYYRAFHVMIVDEIDAFPYHADQMLQYAVQQAM
KERAARIYLTATPDEKWKRNFRRGKQKGIIVSGRYHRHPLPVPLFSWCGNWKKSLHHKKIPRVLLQWLKMYVNKKYPIFL
FVPHVRYIEEMSLLLKGLDHRIDGVHAEDPMRKEKVEAFRKGDIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEEPYGEVVYFHYGKTESMVRAKRHIQSMNKSAKEQGLID

Nucleotide

• Length: 1350 bp

>NTDB_id=130903 AK40_RS22995 WP_000225366.1 3674061..3675410(-) (comFA) [Bacillus cereus 03BB108]
ATGGTTGCTGGAAAGCAGTTACTATTAGAAGAACTCTCTTCAGATTTACGGAGAGAATTAAGTGATTTGAAAAAGAAGGG
AGAGATCATATGTGTACAAGGCGTAATAAAGAAGGCTTCTAAATATATATGTCAGCGCTGCGGAAATATAGAGCAGCGGC
TATTTGCATCATTTTTATGTAAAAGGTGCAGTAAAGTATGTACGTATTGCCGGAAGTGTATAACGATGGGGAGAGTTAGT
GAATGTGCTGTACTTGTTCGCGGAATTCATGAAAGAAAAGGAGAAAGAGAATTACATTCGTTACAGTGGAAAGGAACTTT
GTCTACTGGTCAGGAGTTGGCAGCGCAAGGTGTTATAGAAGCTATTAAGCAGAAAGAATCGTTTTTTATTTGGGCGGTAT
GCGGGGCTGGAAAAACAGAAATGTTATTTTACGGTATAGAAGAGGCACTTCAAAAAGAAGAAAGAGTTTGTATTGCAACG
CCAAGGACGGACGTTGTACTGGAATTAGTACCGAGATTACAAGAAGTGTTTCCAAGTATAAATGTAGCTGCTTTATACGG
AGGGAGTGTAGATCGTGAAAAAGATGCAGCGTTAGTCGTTGCAACGACTCACCAACTGTTACGTTACTATAGAGCATTCC
ATGTGATGATTGTAGATGAGATTGATGCCTTCCCGTATCATGCGGATCAAATGTTACAGTATGCAGTGCAACAAGCGATG
AAAGAGAGAGCGGCGCGTATTTATTTAACTGCAACCCCGGATGAAAAGTGGAAGCGCAATTTCAGAAGGGGGAAACAAAA
AGGTATCATTGTCTCTGGACGATACCATCGTCATCCGTTACCAGTTCCTCTATTTAGTTGGTGCGGTAATTGGAAGAAAA
GCCTTCATCATAAAAAAATTCCTCGAGTGTTACTACAATGGTTGAAAATGTACGTAAACAAAAAATATCCTATTTTTTTA
TTTGTTCCTCATGTGCGATATATAGAAGAAATGAGCCTGTTATTGAAAGGGTTGGATCATAGAATCGATGGCGTACATGC
AGAAGATCCGATGAGAAAAGAAAAAGTGGAAGCGTTTAGAAAGGGAGACATTCCGTTATTAGTTACAACGACAATTTTAG
AAAGAGGGGTAACTGTGAAGAACTTACAAGTGGCTGTGTTAGGGGCTGAAGAAGAAATATTTTCAGAAAGTGCGCTCGTA
CAAATTGCAGGCCGAGCAGGTCGTAGTTTTGAAGAACCGTATGGTGAGGTTGTTTATTTTCACTACGGCAAGACAGAGTC
GATGGTACGTGCGAAAAGACATATTCAAAGTATGAACAAAAGTGCGAAAGAACAAGGATTAATTGATTAA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (107-254)

• Helicase conserved C-terminal domain (Helicase_C) (312-408)

3D structure

• AlphaFold DB: A0A158RMK4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	51.914	93.096	0.483
comFA	Latilactobacillus sakei subsp. sakei 23K	40.5	89.087	0.361