Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EP23_RS23305 Genome accession   NZ_CP009623
Coordinates   2271477..2272847 (+) Length   456 a.a.
NCBI ID   WP_037566299.1    Uniprot ID   A0A2T4MFZ0
Organism   Staphylococcus agnetis strain 908     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2266477..2277847
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EP23_RS23285 (EP23_11120) - 2266812..2267273 (+) 462 WP_037566295.1 CtsR family transcriptional regulator -
  EP23_RS23290 (EP23_11125) - 2267285..2267803 (+) 519 WP_060552317.1 UvrB/UvrC motif-containing protein -
  EP23_RS23295 (EP23_11130) - 2267796..2268800 (+) 1005 WP_060552318.1 protein arginine kinase -
  EP23_RS23300 (EP23_11135) - 2268813..2271275 (+) 2463 WP_060552319.1 ATP-dependent Clp protease ATP-binding subunit -
  EP23_RS23305 (EP23_11140) radA 2271477..2272847 (+) 1371 WP_037566299.1 DNA repair protein RadA Machinery gene
  EP23_RS23310 (EP23_11145) - 2272862..2273923 (+) 1062 WP_060552320.1 PIN/TRAM domain-containing protein -
  EP23_RS23315 (EP23_11150) ispD 2273926..2274621 (+) 696 WP_060552321.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  EP23_RS23320 (EP23_11155) ispF 2274614..2275090 (+) 477 WP_060552322.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  EP23_RS23325 (EP23_11160) gltX 2275179..2276633 (+) 1455 WP_060552323.1 glutamate--tRNA ligase -
  EP23_RS23330 (EP23_11165) cysE 2276963..2277589 (+) 627 WP_168429109.1 serine O-acetyltransferase -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50082.67 Da        Isoelectric Point: 8.0159

>NTDB_id=130846 EP23_RS23305 WP_037566299.1 2271477..2272847(+) (radA) [Staphylococcus agnetis strain 908]
MAKKKITFECTACGYQSPKWMGKCPNCGAWNSMEESLEQKTAHPKHGVRAQKTHAKVQTLNEIKNEQTPRILTKSEEFNR
VLGGGIVQGSLVLIGGDPGIGKSTLLLQMCAALSKTKKVLYITGEESLNQTKLRADRLDEDASALNVFAETDLEVIHDAV
KKVEPELIVVDSIQTIYHPEISSAPGSVSQVRESTQSLMHIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGD
AHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKSVPNPSEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNP
RRMATGIDHNRLSLLMAVLEKKEGYLLQQQDAYIKVAGGVKLSEPAVDLGIIIATASSFKDQAVDGLDCFIGEVGLTGEV
RRVSRIEQRVQEAAKLGFKRVIIPKTNIGGWTFPENIEVIGVSSTNEALKHALKQR

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=130846 EP23_RS23305 WP_037566299.1 2271477..2272847(+) (radA) [Staphylococcus agnetis strain 908]
GTGGCCAAAAAAAAGATTACATTTGAGTGTACTGCATGCGGATACCAGTCTCCAAAATGGATGGGGAAATGCCCAAATTG
CGGTGCATGGAATTCAATGGAAGAATCATTGGAACAAAAAACAGCTCACCCTAAACATGGCGTGCGTGCGCAAAAAACAC
ATGCAAAAGTGCAAACATTAAATGAAATTAAAAATGAACAGACACCACGTATTCTTACTAAAAGTGAAGAATTTAACCGT
GTGCTTGGAGGCGGAATTGTTCAAGGTTCTCTCGTGCTCATCGGGGGCGATCCAGGTATCGGTAAATCAACACTCCTCCT
TCAAATGTGTGCTGCGTTATCTAAAACTAAGAAAGTACTTTATATTACCGGTGAAGAATCGCTAAATCAAACGAAACTAC
GCGCCGACCGTTTAGATGAAGATGCTAGTGCACTCAATGTGTTTGCAGAAACGGATCTTGAAGTCATTCATGATGCAGTA
AAAAAAGTGGAGCCTGAGTTAATCGTTGTAGATTCCATTCAAACCATTTATCACCCAGAAATCAGTTCTGCACCGGGGTC
AGTATCTCAAGTGAGGGAGAGTACACAAAGCCTCATGCATATTGCCAAACAAATGAATATCGCTACATTTATCGTTGGAC
ACGTTACGAAAGAAGGCCAAATTGCGGGTCCTCGATTGTTAGAACATATGGTGGATACTGTGCTCTATTTTGAAGGCGAT
GCACATCATGCATATCGCATTTTGCGTGCGGTGAAGAACCGGTTTGGTTCTACAAATGAAATGGGAATATTTGAAATGAA
ACAATCAGGTTTAAAAAGCGTGCCGAATCCGTCTGAAATGTTTTTAGAAGAACGCTCTACAAATGTACCAGGTTCTACGA
TTGTGGCAACAATGGAAGGAACCCGTCCGTTACTGATTGAAGTGCAAGCTTTAGTCACACCAACAACTTTTAACAATCCT
AGACGAATGGCTACAGGTATAGATCATAATCGCCTAAGTTTGTTAATGGCTGTTTTAGAGAAAAAAGAAGGGTATTTACT
CCAGCAACAAGATGCATATATTAAAGTAGCAGGTGGTGTCAAATTAAGTGAACCCGCTGTTGATTTAGGTATCATTATTG
CGACAGCTTCGAGTTTTAAAGATCAAGCTGTTGATGGATTAGATTGTTTTATAGGTGAAGTAGGTTTAACCGGGGAAGTG
CGCAGAGTCTCCCGTATTGAACAAAGGGTTCAAGAAGCGGCGAAGTTGGGATTCAAACGCGTCATTATTCCGAAAACCAA
TATCGGTGGGTGGACATTCCCAGAAAATATAGAAGTAATCGGTGTGTCTTCAACAAATGAAGCATTAAAGCATGCCTTAA
AACAAAGATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(344-427)

AAA domain (AAA_25)

(62-219)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T4MFZ0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.304

100

0.669
  radA Streptococcus pneumoniae Rx1

59.471

99.561

0.592
  radA Streptococcus pneumoniae D39

59.471

99.561

0.592
  radA Streptococcus pneumoniae R6

59.471

99.561

0.592
  radA Streptococcus pneumoniae TIGR4

59.471

99.561

0.592
  radA Streptococcus mitis NCTC 12261

59.471

99.561

0.592
  radA Streptococcus mitis SK321

59.471

99.561

0.592

Multiple sequence alignment