Detailed information NTG0130753

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: lpt_RS15225
• Genome accession: NZ_CP012019
• Coordinates: 3411834..3414011 (+)
• Length: 725 a.a.
• NCBI ID: WP_042754571.1
• Uniprot ID: -
• Organism: Legionella pneumophila subsp. pneumophila strain Toronto-20052
• Function: DNA puliing through the inner membrane (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3406834..3419011

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
lpt_RS15205 (lpt_15205)	lgt2	3407254..3409164 (-)	1911	WP_027226134.1	Dot/Icm type IV secretion system effector glucosyltransferase Lgt2/LegC8	-
lpt_RS15210 (lpt_15210)	-	3409290..3410045 (-)	756	WP_027226133.1	pteridine reductase	-
lpt_RS15215 (lpt_15215)	-	3410224..3411336 (+)	1113	WP_061784493.1	class I SAM-dependent methyltransferase	-
lpt_RS15220 (lpt_15220)	-	3411339..3411833 (+)	495	WP_011216699.1	6-carboxytetrahydropterin synthase	-
lpt_RS15225 (lpt_15225)	priA	3411834..3414011 (+)	2178	WP_042754571.1	primosomal protein N'	Machinery gene
lpt_RS15230 (lpt_15230)	-	3414008..3414400 (+)	393	WP_015961865.1	thioesterase family protein	-
lpt_RS15235 (lpt_15235)	-	3414518..3414787 (-)	270	WP_013102003.1	hypothetical protein	-
lpt_RS15240 (lpt_15240)	thyA	3414863..3415657 (-)	795	WP_011947748.1	thymidylate synthase	-
lpt_RS15245 (lpt_15245)	lgt	3415654..3416424 (-)	771	WP_027226129.1	prolipoprotein diacylglyceryl transferase	-
lpt_RS15250 (lpt_15250)	-	3416442..3417248 (-)	807	WP_027226128.1	sulfite exporter TauE/SafE family protein	-

Sequence

Protein

• Length: 725 a.a.
• Molecular weight: 81629.04 Da
• Isoelectric Point: 9.2815

>NTDB_id=130753 lpt_RS15225 WP_042754571.1 3411834..3414011(+) (priA) [Legionella pneumophila subsp. pneumophila strain Toronto-20052]
MNDIYQVCIPHTNRDCFDYEALDLTPCIGGRVWVPFRNQARVGIVINKVCGQQTGHSLKKITSIIDEKPMVPEDLLELCL
WVGSYYQSPLSEVIPLVIPKKYRLGLPCSLPTEDFYQLNIPVEKAKTLISKKAKKQHELIDFLSRHQKGISKQTLKQHGY
NGTQIATLLACQAINLSQQVTLPTKIPEKLSPPLTLNPEQAVAVSSILEHIHHYQCFLLQGVTGSGKTEVYLHVIAKILE
QGKQVLVLVPEIGLTPQLLSRFTARFNHPITVLHSNLNESERQIAWQLAKENKVKMVIGTRSAVFTPLPNLGLIIIDEEH
DSSLKQMEGVRYSARDTALMRAHLANIPIILGTATPSLETIHNCKQKKYTLLRLTHKAASTTPLHYQLIDLRSQMVQHGL
ALPTLKIIENHLLQQNQVLVFINRRGFAPVLLCHQCGWMVDCKACDSHLTLHKQAGQMICHHCGLTQRIPLFCNNCQSKE
LIPVGSGTQRIYEFLSHQFPDTNVLRIDRDEVRKKNSLNSHLDKINKGEAQLIIGTQMLAKGHHFPRLSLVVVVDADAGF
YNQDFRAIEHLGQLLTQVSGRAGRAEHPGQVLIQTYLPDHPLLNLLIQHGYDEFANALLISRQEAEMPPFQFLAVIRAQG
KTVNKVDQFLKATKDQIQAHPLTVLGPAPAPLPRKANQHRMQLLIKSPSRKVLKSSLTQLREWLTMNKLSNGIRWNVDVD
PMDLS

Nucleotide

• Length: 2178 bp

>NTDB_id=130753 lpt_RS15225 WP_042754571.1 3411834..3414011(+) (priA) [Legionella pneumophila subsp. pneumophila strain Toronto-20052]
ATGAACGATATTTATCAAGTTTGTATCCCCCATACCAATCGAGATTGTTTTGATTACGAAGCCCTGGATTTAACTCCATG
CATTGGTGGAAGAGTATGGGTTCCTTTTCGCAATCAAGCCCGAGTGGGCATTGTGATCAATAAAGTTTGTGGCCAGCAAA
CGGGCCATTCGTTAAAGAAAATCACTTCAATAATCGATGAGAAACCTATGGTTCCTGAAGATCTCTTAGAGCTTTGTCTA
TGGGTAGGAAGTTATTATCAGTCACCATTATCTGAAGTGATTCCTTTGGTAATACCCAAAAAATATCGCCTGGGTTTGCC
TTGCTCACTACCTACAGAAGATTTTTATCAATTGAATATCCCTGTTGAAAAGGCGAAGACACTCATCTCGAAAAAAGCAA
AAAAACAACATGAATTGATTGATTTTTTAAGCAGACACCAGAAAGGAATTTCCAAACAGACCCTAAAACAACATGGCTAC
AACGGAACTCAAATTGCGACACTACTTGCTTGTCAAGCCATTAACTTGTCACAGCAAGTTACCTTGCCAACCAAAATTCC
TGAAAAACTCAGTCCACCGTTAACACTCAATCCTGAACAAGCCGTTGCCGTATCTTCCATTTTAGAACACATCCATCATT
ACCAATGCTTTTTACTGCAAGGTGTTACCGGAAGTGGTAAGACAGAGGTTTATTTGCATGTCATTGCCAAAATATTGGAG
CAAGGCAAACAAGTATTGGTGCTGGTACCAGAAATCGGGTTAACCCCTCAGCTTTTATCTCGTTTTACAGCCCGATTTAA
CCATCCCATAACCGTATTACATTCCAATTTGAATGAATCAGAAAGGCAAATTGCTTGGCAGCTGGCAAAAGAGAACAAAG
TTAAAATGGTGATTGGAACTCGTTCTGCCGTTTTTACTCCTCTGCCCAATCTCGGTTTAATTATCATCGATGAAGAGCAT
GACAGTTCGCTCAAACAAATGGAGGGGGTGCGTTATTCTGCGCGAGATACTGCTTTAATGCGTGCTCATCTTGCCAATAT
TCCTATCATTCTTGGTACAGCCACTCCCAGTCTGGAAACTATCCACAACTGCAAACAAAAAAAATATACTTTATTACGCT
TAACGCATAAAGCTGCCTCCACTACACCATTACACTATCAACTGATTGATTTAAGATCGCAAATGGTACAGCATGGCTTG
GCTCTCCCAACCCTTAAAATCATAGAAAATCATTTGCTGCAACAAAACCAGGTTCTTGTTTTCATTAACCGACGCGGCTT
TGCCCCAGTGTTACTTTGCCATCAATGTGGATGGATGGTTGATTGCAAAGCATGCGATAGCCATTTAACCTTGCATAAAC
AGGCAGGACAAATGATTTGTCATCATTGCGGATTAACCCAAAGAATACCTTTGTTTTGCAATAACTGCCAAAGCAAAGAA
TTAATCCCCGTAGGATCTGGCACCCAGAGAATTTATGAGTTTTTAAGCCATCAGTTTCCAGATACAAATGTTTTACGAAT
TGACCGAGATGAGGTTCGTAAAAAAAATTCTCTAAACAGCCATTTGGACAAAATTAATAAAGGGGAAGCTCAGCTGATCA
TAGGCACTCAAATGCTAGCCAAGGGACACCATTTCCCCCGCCTATCCCTGGTCGTTGTCGTAGATGCTGATGCAGGCTTT
TACAACCAGGATTTCCGGGCTATTGAACATTTGGGGCAATTATTAACTCAAGTATCTGGACGAGCTGGGCGAGCAGAACA
TCCCGGACAAGTATTGATTCAAACTTATTTACCTGATCATCCCCTGCTTAATCTACTCATTCAGCATGGGTATGATGAAT
TTGCAAACGCTTTATTAATTTCAAGACAAGAGGCTGAAATGCCCCCTTTTCAGTTTTTAGCGGTCATCAGAGCTCAGGGC
AAAACCGTCAACAAGGTGGATCAATTCCTAAAAGCAACCAAAGATCAAATTCAGGCTCATCCTCTCACGGTACTGGGTCC
TGCGCCAGCTCCTTTGCCACGAAAAGCTAACCAGCATAGGATGCAACTATTAATTAAATCCCCTTCAAGAAAAGTTCTAA
AAAGTTCATTGACGCAATTAAGAGAGTGGTTGACAATGAACAAATTAAGTAATGGCATCCGCTGGAATGTGGATGTTGAT
CCTATGGACTTATCATGA

Domains

Predicted by InterProScan.

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (442-468)

• Primosomal protein N C-terminal domain (PriA_C) (629-721)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (6-99)

• Type III restriction enzyme, res subunit (ResIII) (195-357)

• Helicase conserved C-terminal domain (Helicase_C) (489-585)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Acinetobacter baumannii D1279779	42.49	100	0.433