Detailed information NTG0130641

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: BF33_RS23855
• Genome accession: NZ_CP009605
• Coordinates: 4496471..4497820 (+)
• Length: 449 a.a.
• NCBI ID: WP_033688222.1
• Uniprot ID: -
• Organism: Bacillus cereus strain S2-8
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4491471..4502820

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BF33_RS23830 (BF33_4626)	-	4492588..4493223 (-)	636	WP_000926685.1	YigZ family protein	-
BF33_RS23835 (BF33_4627)	-	4493463..4494305 (+)	843	WP_000844766.1	DegV family protein	-
BF33_RS23845 (BF33_4628)	-	4494479..4494793 (+)	315	WP_000400857.1	winged helix-turn-helix transcriptional regulator	-
BF33_RS23850 (BF33_4629)	-	4495046..4496344 (+)	1299	WP_000734327.1	NlpC/P60 family protein	-
BF33_RS23855 (BF33_4630)	comFA	4496471..4497820 (+)	1350	WP_033688222.1	ATP-dependent helicase ComFA	Machinery gene
BF33_RS23860 (BF33_4631)	comFC	4497820..4498524 (+)	705	WP_033688220.1	comF operon protein ComFC	-
BF33_RS23865 (BF33_4632)	cspC	4498651..4498848 (+)	198	WP_001990088.1	cold shock protein CspC	-
BF33_RS23870 (BF33_4633)	hpf	4499173..4499715 (+)	543	WP_000671189.1	ribosome hibernation-promoting factor, HPF/YfiA family	-
BF33_RS23875 (BF33_4634)	secA	4499992..4502499 (+)	2508	WP_000579377.1	preprotein translocase subunit SecA	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 51279.96 Da
• Isoelectric Point: 9.7211

>NTDB_id=130641 BF33_RS23855 WP_033688222.1 4496471..4497820(+) (comFA) [Bacillus cereus strain S2-8]
MLAGKQLLLEELSSDLRRELSDLKKRGEIICVQGVIKKASKYICQRCGNIEQRLFASFLCKRCSKVCTYCRKCITMGRVS
ECTVLVRGIHERKGERELHSLQWKGALTTGQELAAQGVIKAIQQKESFFIWAVCGAGKTEMLFYGIEEALQKGERVCIAT
PRTDVVLELVPRLQEVFPSINVAALYGGSVDREKDAALVVATTHQLLRYYRAFHVMIVDEIDAFPYHADQMLQYAVQQAM
KEKAARIYLTATPDEKWKRNFRTGKQKGIIVSGRYHRHPLPVPLFSWCGNWKKSLHHKKIPRVLLQWLKMYVNKKYPIFL
FVPHVRYIEEIGLLLKGLDHRIDGVHAEDPMRKEKVEAFRKGDIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEEPYGEVVYFHYGKTESMVRAKRHIQSMNKSAKEQGLID

Nucleotide

• Length: 1350 bp

>NTDB_id=130641 BF33_RS23855 WP_033688222.1 4496471..4497820(+) (comFA) [Bacillus cereus strain S2-8]
ATGCTCGCTGGAAAGCAGTTACTATTAGAAGAACTCTCTTCAGATTTACGGAGAGAATTAAGTGATTTGAAAAAGAGGGG
AGAGATCATATGTGTACAAGGCGTAATAAAGAAGGCTTCTAAATATATATGTCAGCGCTGCGGAAATATAGAGCAGCGGC
TATTTGCATCATTTTTATGTAAAAGGTGCAGTAAAGTATGTACGTATTGCCGGAAGTGTATAACGATGGGGAGAGTTAGT
GAATGTACTGTACTTGTTCGCGGGATTCATGAAAGAAAAGGCGAAAGGGAATTACATTCGTTACAGTGGAAAGGTGCATT
GACTACTGGTCAGGAGTTGGCGGCGCAAGGCGTCATAAAAGCGATTCAGCAAAAAGAATCTTTTTTTATTTGGGCTGTGT
GCGGAGCTGGAAAAACAGAGATGTTATTTTACGGTATAGAAGAGGCACTTCAAAAAGGAGAAAGAGTTTGTATCGCAACG
CCAAGGACGGACGTTGTACTGGAATTAGTACCGAGATTACAAGAAGTGTTTCCAAGTATAAATGTAGCTGCTTTATACGG
AGGGAGTGTAGATCGTGAAAAAGATGCAGCGTTAGTCGTTGCAACGACGCATCAATTGTTACGTTATTATAGAGCGTTTC
ATGTAATGATTGTAGATGAGATTGATGCCTTCCCGTATCATGCGGATCAAATGTTACAGTATGCAGTGCAGCAAGCGATG
AAAGAGAAAGCAGCGCGTATTTATTTAACTGCAACCCCTGATGAAAAGTGGAAGCGCAATTTCAGAACGGGGAAACAAAA
AGGTATCATTGTTTCGGGACGATACCATCGTCATCCGTTACCAGTTCCTCTATTTAGTTGGTGCGGAAATTGGAAGAAAA
GCCTACATCATAAAAAAATTCCTCGTGTGTTACTACAATGGTTAAAAATGTACGTAAACAAAAAATACCCTATTTTTTTA
TTTGTTCCTCATGTGCGATATATAGAAGAAATAGGCCTGTTATTGAAAGGGTTGGATCATAGAATCGATGGCGTACATGC
AGAAGATCCGATGAGAAAAGAAAAAGTGGAAGCGTTTCGAAAGGGAGACATTCCGTTATTAGTTACAACGACAATTTTAG
AAAGAGGGGTAACTGTGAAGAACTTACAAGTGGCTGTGTTAGGGGCTGAAGAAGAAATATTTTCAGAAAGTGCGCTCGTA
CAAATTGCAGGCCGAGCAGGTCGTAGTTTTGAAGAACCGTATGGTGAGGTTGTTTATTTTCATTACGGTAAGACAGAGTC
GATGGTACGTGCGAAAAGACATATTCAAAGTATGAACAAAAGTGCGAAAGAACAAGGATTAATTGATTAA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (107-254)

• Helicase conserved C-terminal domain (Helicase_C) (312-408)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	51.675	93.096	0.481
comFA	Latilactobacillus sakei subsp. sakei 23K	40.955	88.641	0.363