Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   BF33_RS14195 Genome accession   NZ_CP009605
Coordinates   2653974..2654576 (+) Length   200 a.a.
NCBI ID   WP_000155594.1    Uniprot ID   Q81SS2
Organism   Bacillus cereus strain S2-8     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2648974..2659576
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BF33_RS14155 (BF33_2706) gpsB 2649355..2649693 (-) 339 WP_000622430.1 cell division regulator GpsB -
  BF33_RS14160 (BF33_2707) - 2649791..2650345 (-) 555 WP_000862921.1 DUF1273 domain-containing protein -
  BF33_RS14165 (BF33_2708) - 2650402..2650827 (-) 426 WP_000546265.1 CotD family spore coat protein -
  BF33_RS14170 (BF33_2709) - 2650983..2651462 (-) 480 WP_000487705.1 YppG family protein -
  BF33_RS14175 (BF33_2710) - 2651640..2651831 (+) 192 WP_000242663.1 YppF family protein -
  BF33_RS14180 (BF33_2711) - 2651857..2652201 (-) 345 WP_000872959.1 YppE family protein -
  BF33_RS14185 (BF33_2712) - 2652217..2652459 (-) 243 WP_001252043.1 hypothetical protein -
  BF33_RS31565 - 2652545..2652721 (+) 177 WP_001982922.1 hypothetical protein -
  BF33_RS14190 (BF33_2714) - 2652845..2653870 (-) 1026 WP_000495642.1 DUF2515 domain-containing protein -
  BF33_RS14195 (BF33_2715) recU 2653974..2654576 (+) 603 WP_000155594.1 Holliday junction resolvase RecU Machinery gene
  BF33_RS14200 (BF33_2716) - 2654642..2657335 (+) 2694 WP_001283116.1 PBP1A family penicillin-binding protein -
  BF33_RS14205 (BF33_2717) - 2657359..2657877 (-) 519 WP_000442686.1 YpoC family protein -
  BF33_RS14210 (BF33_2718) nth 2657881..2658528 (-) 648 WP_000933025.1 endonuclease III -
  BF33_RS14215 (BF33_2719) dnaD 2658544..2659251 (-) 708 WP_000728549.1 DNA replication protein DnaD -

Sequence

Protein


Download         Length: 200 a.a.        Molecular weight: 23342.66 Da        Isoelectric Point: 9.5601

>NTDB_id=130616 BF33_RS14195 WP_000155594.1 2653974..2654576(+) (recU) [Bacillus cereus strain S2-8]
MTIRYPNGKRYNQASQPHKTPIKKHTYSNRGMSLEEELNETNEYYLTHNIACVHKKPTPLQIVKVDYPARSAAVVKEAYF
KQPSTTDYNGVYKGKYIDFEAKETKNKTSFPLQNFHLHQIEHMKQVIAHNGIAFVIIKFTLFDELYLLDAKHIITFWNRQ
NTGGRKSITKEEIVEHGSLLSCGYHPRIDYIRVLDTVYFS

Nucleotide


Download         Length: 603 bp        

>NTDB_id=130616 BF33_RS14195 WP_000155594.1 2653974..2654576(+) (recU) [Bacillus cereus strain S2-8]
ATGACCATTCGTTACCCAAATGGAAAACGGTACAATCAAGCTTCACAACCTCATAAAACACCAATTAAAAAACATACTTA
TAGTAACAGAGGTATGTCCCTTGAAGAGGAATTGAATGAAACAAATGAATATTACTTAACCCATAATATTGCATGTGTAC
ATAAAAAACCTACACCTCTTCAAATTGTAAAAGTAGATTACCCCGCTCGAAGTGCTGCAGTGGTAAAAGAGGCGTATTTT
AAACAACCTTCTACAACAGATTACAACGGTGTATATAAAGGGAAATACATCGATTTTGAAGCGAAAGAAACAAAAAATAA
AACCAGCTTTCCACTTCAAAACTTCCACCTTCATCAAATTGAACATATGAAGCAAGTAATTGCTCATAATGGAATTGCAT
TTGTTATTATTAAATTTACACTTTTTGATGAACTTTATTTATTAGATGCAAAACATATTATTACATTTTGGAATCGTCAA
AATACTGGTGGACGAAAATCGATTACGAAAGAAGAAATAGTAGAGCATGGATCTTTATTATCATGCGGTTATCACCCTCG
GATTGATTATATCCGTGTACTAGACACGGTTTATTTTTCGTGA

Domains

Predicted by InterproScan.

Recombination protein U (RecU)

(30-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q81SS2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

62.5

100

0.625

Multiple sequence alignment