Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   BF33_RS00655 Genome accession   NZ_CP009605
Coordinates   41101..41847 (+) Length   248 a.a.
NCBI ID   WP_000487010.1    Uniprot ID   Q81LT9
Organism   Bacillus cereus strain S2-8     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 36101..46847
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BF33_RS00625 (BF33_42) - 36390..38534 (+) 2145 WP_000946454.1 HD family phosphohydrolase -
  BF33_RS00630 (BF33_43) ybeY 38531..39001 (+) 471 WP_000054692.1 rRNA maturation RNase YbeY -
  BF33_RS00635 (BF33_44) - 38998..39351 (+) 354 WP_000715480.1 diacylglycerol kinase family protein -
  BF33_RS00640 (BF33_45) - 39451..39849 (+) 399 WP_001086294.1 cytidine deaminase -
  BF33_RS00645 (BF33_46) era 39842..40747 (+) 906 WP_001080241.1 GTPase Era -
  BF33_RS00650 (BF33_47) - 40924..41067 (+) 144 WP_000883924.1 YqzL family protein -
  BF33_RS00655 (BF33_48) recO 41101..41847 (+) 747 WP_000487010.1 DNA repair protein RecO Machinery gene
  BF33_RS00660 (BF33_49) - 41955..42587 (+) 633 WP_000583759.1 helix-turn-helix transcriptional regulator -
  BF33_RS00665 (BF33_50) - 42617..43429 (+) 813 WP_000368943.1 pyruvate, water dikinase regulatory protein -
  BF33_RS31400 - 43797..43952 (+) 156 WP_001179661.1 hypothetical protein -
  BF33_RS00680 (BF33_51) dnaG 44283..46079 (+) 1797 WP_000528216.1 DNA primase -

Sequence

Protein


Download         Length: 248 a.a.        Molecular weight: 28665.22 Da        Isoelectric Point: 8.0540

>NTDB_id=130605 BF33_RS00655 WP_000487010.1 41101..41847(+) (recO) [Bacillus cereus strain S2-8]
MFQKVEGIVIRTTDYGETNKIVTIFSRELGKVSAMARGAKKPKSRLASVSQLMTHGHFLIQMGSGLGTLQQGEIISTMKE
IREDIFLTAYASFIVELTDKATEDKKHNPYLFEMLYQTLHYMCEGVDPEVLSLIYQTKMLPVLGMRPYFDTCAICHQETD
FVAFSVREGGFLCSRHAEQDQYRIPVGEAVHKLLRLFYHFDLHRLGNVSVKDSTKKQMRLVLNTYYDEYCGIYLKSRRFL
EQLDKFQI

Nucleotide


Download         Length: 747 bp        

>NTDB_id=130605 BF33_RS00655 WP_000487010.1 41101..41847(+) (recO) [Bacillus cereus strain S2-8]
ATGTTTCAAAAAGTTGAGGGCATCGTTATCCGTACGACAGATTACGGAGAAACGAACAAGATTGTTACAATATTTTCAAG
AGAATTGGGTAAGGTAAGTGCAATGGCAAGAGGAGCGAAAAAACCGAAAAGTCGGTTAGCATCTGTTTCGCAACTTATGA
CACATGGTCATTTTCTTATTCAAATGGGATCTGGACTTGGAACTTTGCAACAAGGCGAGATTATTTCAACTATGAAAGAA
ATTCGCGAGGATATATTTTTAACTGCTTATGCATCATTTATTGTTGAATTAACTGATAAAGCAACAGAAGATAAAAAACA
TAATCCATATTTATTTGAAATGTTATATCAAACGTTGCATTATATGTGTGAGGGTGTTGATCCAGAAGTATTATCATTAA
TTTATCAAACGAAAATGCTTCCGGTATTAGGGATGCGCCCATACTTTGACACATGTGCGATTTGTCATCAAGAAACAGAT
TTTGTCGCCTTCTCTGTTAGAGAGGGCGGTTTTCTGTGCTCACGTCATGCTGAGCAAGATCAGTATCGTATACCAGTGGG
GGAAGCCGTTCATAAATTATTGCGTCTTTTCTACCACTTCGATTTACATAGACTTGGCAATGTATCAGTGAAGGATAGCA
CAAAAAAACAAATGCGTTTAGTATTGAATACATATTATGATGAATATTGCGGGATTTATTTGAAATCAAGACGTTTCTTA
GAACAACTTGATAAGTTTCAAATATAA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-238)

Recombination protein O N terminal (RecO_N)

(1-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q81LT9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

55.466

99.597

0.552

Multiple sequence alignment