Detailed information NTG0130566

Bioinformatically predicted

Overview

• Name: recF
• Type: Machinery gene
• Locus tag: BF32_RS15995
• Genome accession: NZ_CP009600
• Coordinates: 2998680..2999807 (+)
• Length: 375 a.a.
• NCBI ID: WP_000470750.1
• Uniprot ID: A0A2C3GXQ0
• Organism: Bacillus thuringiensis strain HD571
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 2993680..3004807

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BF32_RS15965 (BF32_3086)	spoIIIJ	2993726..2994493 (-)	768	WP_000727748.1	YidC family membrane integrase SpoIIIJ	-
BF32_RS15970 (BF32_3087)	rnpA	2994533..2994892 (-)	360	WP_000726628.1	ribonuclease P protein component	-
BF32_RS15975 (BF32_3088)	rpmH	2994969..2995103 (-)	135	WP_000831901.1	50S ribosomal protein L34	-
BF32_RS15980 (BF32_3089)	dnaA	2995669..2997009 (+)	1341	WP_000428021.1	chromosomal replication initiator protein DnaA	-
BF32_RS15985 (BF32_3090)	dnaN	2997188..2998327 (+)	1140	WP_001212527.1	DNA polymerase III subunit beta	-
BF32_RS15990 (BF32_3091)	yaaA	2998455..2998667 (+)	213	WP_000821367.1	S4 domain-containing protein YaaA	-
BF32_RS15995 (BF32_3092)	recF	2998680..2999807 (+)	1128	WP_000470750.1	DNA replication/repair protein RecF	Machinery gene
BF32_RS16000 (BF32_3093)	gyrB	2999846..3001768 (+)	1923	WP_000435993.1	DNA topoisomerase (ATP-hydrolyzing) subunit B	-
BF32_RS16005 (BF32_3094)	gyrA	3001857..3004328 (+)	2472	WP_001282851.1	DNA gyrase subunit A	-

Sequence

Protein

• Length: 375 a.a.
• Molecular weight: 43334.66 Da
• Isoelectric Point: 6.7809

>NTDB_id=130566 BF32_RS15995 WP_000470750.1 2998680..2999807(+) (recF) [Bacillus thuringiensis strain HD571]
MFISEIQLKNYRNYEKLELSFEDKVNVIIGENAQGKTNLMEAIYVLAMAKSHRTSNDRELIRWDEDFGQIKGKLQKRNSS
LSLELNISKKGKKAKLNQLEQQKLSQYIGVMNVVMFAPEDLNLVKGSPQVRRRFLDMELGQIAPVYLYELSQYQKVLTQR
NHLLKKMQGNSKNEETMLDVFTLQLIEHGTKILQKRFEFLHLLQEWAAPIHRGISRGLEELEIVYKPSVDVSESMDLSKI
KEVYYESFQSVKQREIFRGTTLIGPHRDDLQFFVNSKNVQVFGSQGQQRTTALSLKLAEIELIYSEVKEYPILLLDDVLS
ELDDYRQSHLLNTIQGKVQTFVTTTSVDGIEHETLKEAKTIHVTNGTVDCEIDRA

Nucleotide

• Length: 1128 bp

>NTDB_id=130566 BF32_RS15995 WP_000470750.1 2998680..2999807(+) (recF) [Bacillus thuringiensis strain HD571]
TTGTTTATTTCAGAAATACAATTAAAAAACTATCGCAATTATGAAAAATTAGAGCTTTCCTTTGAAGATAAAGTAAATGT
AATTATCGGCGAGAACGCGCAAGGGAAAACGAATTTGATGGAAGCTATTTATGTTTTGGCGATGGCGAAATCTCATAGAA
CCTCTAATGATCGTGAGCTTATCCGTTGGGATGAAGATTTCGGTCAAATTAAAGGAAAGTTACAAAAGAGAAATAGTTCT
TTGTCTTTGGAATTAAATATCTCGAAAAAAGGTAAGAAGGCAAAATTAAATCAACTTGAACAACAAAAATTGAGTCAATA
TATTGGCGTGATGAATGTTGTCATGTTTGCCCCAGAAGATTTAAATCTTGTAAAAGGAAGCCCTCAAGTAAGAAGACGCT
TTTTAGATATGGAATTAGGCCAAATAGCTCCGGTCTATTTGTATGAATTGAGTCAATATCAAAAGGTGCTCACACAACGA
AATCACTTGCTGAAAAAGATGCAAGGGAATAGTAAGAATGAGGAAACGATGTTGGATGTATTTACACTTCAACTTATTGA
GCATGGTACAAAAATATTGCAAAAACGCTTTGAGTTTTTGCATTTACTACAAGAATGGGCAGCTCCAATTCATCGTGGTA
TAAGCCGTGGATTAGAAGAATTAGAAATTGTCTATAAACCGAGTGTAGATGTATCAGAATCAATGGATTTGTCGAAAATA
AAAGAAGTATACTATGAAAGTTTTCAATCAGTGAAACAACGTGAAATTTTCCGTGGTACGACTTTAATTGGTCCTCATCG
TGATGATTTACAATTCTTCGTTAATAGTAAAAATGTTCAAGTCTTTGGTTCGCAAGGACAACAACGAACGACCGCACTGT
CCCTAAAATTAGCTGAAATTGAATTGATTTATTCAGAAGTTAAGGAATATCCGATTCTTTTACTCGATGATGTATTATCA
GAATTAGATGATTATCGTCAATCGCATCTGTTAAATACAATTCAAGGAAAAGTGCAAACATTTGTGACAACGACGAGTGT
CGACGGAATTGAACACGAAACATTAAAAGAAGCGAAAACAATTCATGTAACGAACGGCACGGTAGATTGTGAAATAGATA
GGGCGTAA

Domains

Predicted by InterproScan.

• RecF/RecN/SMC N terminal domain (SMC_N) (2-348)

3D structure

• AlphaFold DB: A0A2C3GXQ0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recF	Bacillus subtilis subsp. subtilis str. 168	66.304	98.133	0.651