Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   BF32_RS09945 Genome accession   NZ_CP009600
Coordinates   1838848..1839594 (-) Length   248 a.a.
NCBI ID   WP_000487010.1    Uniprot ID   Q81LT9
Organism   Bacillus thuringiensis strain HD571     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 1833848..1844594
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BF32_RS09920 (BF32_1888) dnaG 1834616..1836412 (-) 1797 WP_000528216.1 DNA primase -
  BF32_RS29575 - 1836743..1836898 (-) 156 WP_001179661.1 hypothetical protein -
  BF32_RS09935 (BF32_1889) - 1837266..1838078 (-) 813 WP_000368943.1 pyruvate, water dikinase regulatory protein -
  BF32_RS09940 (BF32_1890) - 1838108..1838740 (-) 633 WP_000583759.1 helix-turn-helix transcriptional regulator -
  BF32_RS09945 (BF32_1891) recO 1838848..1839594 (-) 747 WP_000487010.1 DNA repair protein RecO Machinery gene
  BF32_RS09950 (BF32_1892) - 1839628..1839771 (-) 144 WP_000883924.1 YqzL family protein -
  BF32_RS09955 (BF32_1893) era 1839948..1840853 (-) 906 WP_001080241.1 GTPase Era -
  BF32_RS09960 (BF32_1894) - 1840846..1841244 (-) 399 WP_001086294.1 cytidine deaminase -
  BF32_RS09965 (BF32_1895) - 1841344..1841697 (-) 354 WP_000715480.1 diacylglycerol kinase family protein -
  BF32_RS09970 (BF32_1896) ybeY 1841694..1842164 (-) 471 WP_000054692.1 rRNA maturation RNase YbeY -
  BF32_RS09975 (BF32_1897) - 1842161..1844305 (-) 2145 WP_000946454.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 248 a.a.        Molecular weight: 28665.22 Da        Isoelectric Point: 8.0540

>NTDB_id=130550 BF32_RS09945 WP_000487010.1 1838848..1839594(-) (recO) [Bacillus thuringiensis strain HD571]
MFQKVEGIVIRTTDYGETNKIVTIFSRELGKVSAMARGAKKPKSRLASVSQLMTHGHFLIQMGSGLGTLQQGEIISTMKE
IREDIFLTAYASFIVELTDKATEDKKHNPYLFEMLYQTLHYMCEGVDPEVLSLIYQTKMLPVLGMRPYFDTCAICHQETD
FVAFSVREGGFLCSRHAEQDQYRIPVGEAVHKLLRLFYHFDLHRLGNVSVKDSTKKQMRLVLNTYYDEYCGIYLKSRRFL
EQLDKFQI

Nucleotide


Download         Length: 747 bp        

>NTDB_id=130550 BF32_RS09945 WP_000487010.1 1838848..1839594(-) (recO) [Bacillus thuringiensis strain HD571]
ATGTTTCAAAAAGTTGAGGGCATCGTTATCCGTACGACAGATTACGGAGAAACGAACAAGATTGTTACAATATTTTCAAG
AGAATTGGGTAAGGTAAGCGCAATGGCAAGAGGAGCGAAAAAACCGAAAAGCCGGTTAGCATCTGTTTCGCAACTTATGA
CACATGGTCATTTTCTTATTCAAATGGGATCTGGACTTGGAACTTTGCAACAAGGCGAGATTATTTCAACTATGAAAGAA
ATTCGCGAGGATATATTTTTAACTGCTTATGCATCATTTATTGTTGAATTAACTGATAAAGCAACAGAAGATAAAAAACA
TAATCCATATTTATTTGAAATGTTATATCAAACGTTGCATTATATGTGTGAGGGTGTTGATCCGGAAGTATTATCATTAA
TTTATCAAACGAAAATGCTTCCGGTATTAGGGATGCGCCCGTACTTTGACACATGTGCGATTTGTCATCAAGAAACAGAT
TTTGTCGCCTTCTCTGTTAGAGAGGGCGGTTTTCTGTGCTCACGTCATGCTGAGCAAGATCAGTATCGTATACCAGTGGG
GGAAGCCGTTCATAAATTATTGCGTCTTTTCTACCACTTCGATTTACATAGACTTGGCAATGTATCAGTGAAGGATAGCA
CAAAAAAACAAATGCGTTTAGTATTGAATACATATTATGATGAATATTGCGGGATTTATTTGAAATCAAGACGTTTCTTA
GAACAACTTGATAAGTTTCAAATATAA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-238)

Recombination protein O N terminal (RecO_N)

(1-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q81LT9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

55.466

99.597

0.552

Multiple sequence alignment