Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BF32_RS08450 Genome accession   NZ_CP009600
Coordinates   1587262..1587696 (-) Length   144 a.a.
NCBI ID   WP_000811492.1    Uniprot ID   A0A0G8EA73
Organism   Bacillus thuringiensis strain HD571     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1582262..1592696
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BF32_RS08430 (BF32_1604) - 1583026..1583583 (+) 558 WP_000062080.1 PadR family transcriptional regulator -
  BF32_RS08440 (BF32_1605) metE 1584511..1586799 (+) 2289 WP_001007632.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  BF32_RS08445 (BF32_1606) comJ 1586836..1587225 (-) 390 WP_000424033.1 competence protein ComJ -
  BF32_RS08450 (BF32_1607) nucA/comI 1587262..1587696 (-) 435 WP_000811492.1 DNA-entry nuclease Machinery gene
  BF32_RS08455 (BF32_1608) - 1587933..1588682 (+) 750 WP_000388464.1 DUF3967 domain-containing protein -
  BF32_RS08460 (BF32_1609) kinB 1588736..1590010 (-) 1275 WP_000420389.1 sporulation sensor histidine kinase KinB -
  BF32_RS08465 (BF32_1610) - 1590321..1590521 (+) 201 WP_000929284.1 hypothetical protein -
  BF32_RS08470 (BF32_1611) - 1590644..1591807 (+) 1164 WP_000434590.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16012.35 Da        Isoelectric Point: 8.4331

>NTDB_id=130546 BF32_RS08450 WP_000811492.1 1587262..1587696(-) (nucA/comI) [Bacillus thuringiensis strain HD571]
MKQLKGIIISIIAILSILVAVYEVLVPEETSTKKTNAYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=130546 BF32_RS08450 WP_000811492.1 1587262..1587696(-) (nucA/comI) [Bacillus thuringiensis strain HD571]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTTTAGTAGCGGTTTATGAAGTACTTGTTCC
AGAGGAAACAAGTACAAAAAAAACAAATGCGTATGATCAAGTTCTAGAATTTCCGAAAGAGAGATATCCAGAAACAGGGA
AACATATTACGGATGCGATAAAGGAAGGACATTCAGAAGTTTGTACAATCGACCGTGGTGGTGCTGCGGATAGAAGAAAG
TTATCGTTAGCTCCGTACCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGAGCACATATCGAATATATAAGTCCAGCAGATAACCGCGGAGCAGGGTCTTGGGTAGGAAATAAGTTAGATAAATACC
CAGATGGTACGCGTGTGAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0G8EA73

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

70.796

78.472

0.556

Multiple sequence alignment