Detailed information NTG0130476

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: BG11_RS22220
• Genome accession: NZ_CP009596
• Coordinates: 3951648..3952997 (+)
• Length: 449 a.a.
• NCBI ID: WP_033688222.1
• Uniprot ID: -
• Organism: Bacillus cereus strain 3a
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3946648..3957997

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BG11_RS22195 (BG11_4040)	-	3947765..3948400 (-)	636	WP_000926685.1	YigZ family protein	-
BG11_RS22200 (BG11_4041)	-	3948640..3949482 (+)	843	WP_000844766.1	DegV family protein	-
BG11_RS22210 (BG11_4042)	-	3949656..3949970 (+)	315	WP_000400857.1	winged helix-turn-helix transcriptional regulator	-
BG11_RS22215 (BG11_4043)	-	3950223..3951521 (+)	1299	WP_000734327.1	NlpC/P60 family protein	-
BG11_RS22220 (BG11_4044)	comFA	3951648..3952997 (+)	1350	WP_033688222.1	ATP-dependent helicase ComFA	Machinery gene
BG11_RS22225 (BG11_4045)	comFC	3952997..3953701 (+)	705	WP_033688220.1	comF operon protein ComFC	-
BG11_RS22230 (BG11_4046)	cspC	3953828..3954025 (+)	198	WP_001990088.1	cold shock protein CspC	-
BG11_RS22235 (BG11_4047)	hpf	3954350..3954892 (+)	543	WP_000671189.1	ribosome hibernation-promoting factor, HPF/YfiA family	-
BG11_RS22240 (BG11_4048)	secA	3955169..3957676 (+)	2508	WP_000579377.1	preprotein translocase subunit SecA	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 51279.96 Da
• Isoelectric Point: 9.7211

>NTDB_id=130476 BG11_RS22220 WP_033688222.1 3951648..3952997(+) (comFA) [Bacillus cereus strain 3a]
MLAGKQLLLEELSSDLRRELSDLKKRGEIICVQGVIKKASKYICQRCGNIEQRLFASFLCKRCSKVCTYCRKCITMGRVS
ECTVLVRGIHERKGERELHSLQWKGALTTGQELAAQGVIKAIQQKESFFIWAVCGAGKTEMLFYGIEEALQKGERVCIAT
PRTDVVLELVPRLQEVFPSINVAALYGGSVDREKDAALVVATTHQLLRYYRAFHVMIVDEIDAFPYHADQMLQYAVQQAM
KEKAARIYLTATPDEKWKRNFRTGKQKGIIVSGRYHRHPLPVPLFSWCGNWKKSLHHKKIPRVLLQWLKMYVNKKYPIFL
FVPHVRYIEEIGLLLKGLDHRIDGVHAEDPMRKEKVEAFRKGDIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEEPYGEVVYFHYGKTESMVRAKRHIQSMNKSAKEQGLID

Nucleotide

• Length: 1350 bp

>NTDB_id=130476 BG11_RS22220 WP_033688222.1 3951648..3952997(+) (comFA) [Bacillus cereus strain 3a]
ATGCTCGCTGGAAAGCAGTTACTATTAGAAGAACTCTCTTCAGATTTACGGAGAGAATTAAGTGATTTGAAAAAGAGGGG
AGAGATCATATGTGTACAAGGCGTAATAAAGAAGGCTTCTAAATATATATGTCAGCGCTGCGGAAATATAGAGCAGCGGC
TATTTGCATCATTTTTATGTAAAAGGTGCAGTAAAGTATGTACGTATTGCCGGAAGTGTATAACGATGGGGAGAGTTAGT
GAATGTACTGTACTTGTTCGCGGGATTCATGAAAGAAAAGGCGAAAGGGAATTACATTCGTTACAGTGGAAAGGTGCATT
GACTACTGGTCAGGAGTTGGCGGCGCAAGGCGTCATAAAAGCGATTCAGCAAAAAGAATCTTTTTTTATTTGGGCTGTGT
GCGGAGCTGGAAAAACAGAGATGTTATTTTACGGTATAGAAGAGGCACTTCAAAAAGGAGAAAGAGTTTGTATCGCAACG
CCAAGGACGGACGTTGTACTGGAATTAGTACCGAGATTACAAGAAGTGTTTCCAAGTATAAATGTAGCTGCTTTATACGG
AGGGAGTGTAGATCGTGAAAAAGATGCAGCGTTAGTCGTTGCAACGACGCATCAATTGTTACGTTATTATAGAGCGTTTC
ATGTAATGATTGTAGATGAGATTGATGCCTTCCCGTATCATGCGGATCAAATGTTACAGTATGCAGTGCAGCAAGCGATG
AAAGAGAAAGCAGCGCGTATTTATTTAACTGCAACCCCTGATGAAAAGTGGAAGCGCAATTTCAGAACGGGGAAACAAAA
AGGTATCATTGTTTCGGGACGATACCATCGTCATCCGTTACCAGTTCCTCTATTTAGTTGGTGCGGAAATTGGAAGAAAA
GCCTACATCATAAAAAAATTCCTCGTGTGTTACTACAATGGTTAAAAATGTACGTAAACAAAAAATACCCTATTTTTTTA
TTTGTTCCTCATGTGCGATATATAGAAGAAATAGGCCTGTTATTGAAAGGGTTGGATCATAGAATCGATGGCGTACATGC
AGAAGATCCGATGAGAAAAGAAAAAGTGGAAGCGTTTCGAAAGGGAGACATTCCGTTATTAGTTACAACGACAATTTTAG
AAAGAGGGGTAACTGTGAAGAACTTACAAGTGGCTGTGTTAGGGGCTGAAGAAGAAATATTTTCAGAAAGTGCGCTCGTA
CAAATTGCAGGCCGAGCAGGTCGTAGTTTTGAAGAACCGTATGGTGAGGTTGTTTATTTTCATTACGGTAAGACAGAGTC
GATGGTACGTGCGAAAAGACATATTCAAAGTATGAACAAAAGTGCGAAAGAACAAGGATTAATTGATTAA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (107-254)

• Helicase conserved C-terminal domain (Helicase_C) (312-408)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	51.675	93.096	0.481
comFA	Latilactobacillus sakei subsp. sakei 23K	40.955	88.641	0.363