Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BW21_RS30245 Genome accession   NZ_CP009549
Coordinates   3749438..3750946 (+) Length   502 a.a.
NCBI ID   WP_006024494.1    Uniprot ID   A0AAU8SQX0
Organism   Burkholderia sp. 2002721687     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3744438..3755946
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BW21_RS30220 (BW21_3358) kdsD 3745671..3746654 (+) 984 WP_006024499.1 arabinose 5-phosphate isomerase KdsD -
  BW21_RS30225 (BW21_3359) - 3746654..3747193 (+) 540 WP_009911188.1 KdsC family phosphatase -
  BW21_RS30230 (BW21_3360) lptC 3747198..3747812 (+) 615 WP_006024497.1 LPS export ABC transporter periplasmic protein LptC -
  BW21_RS30235 (BW21_3361) lptA 3747831..3748496 (+) 666 WP_006024496.1 lipopolysaccharide transport periplasmic protein LptA -
  BW21_RS30240 (BW21_3362) lptB 3748493..3749269 (+) 777 WP_006024495.1 LPS export ABC transporter ATP-binding protein -
  BW21_RS30245 (BW21_3364) rpoN/rpoN1 3749438..3750946 (+) 1509 WP_006024494.1 RNA polymerase factor sigma-54 Machinery gene
  BW21_RS30250 (BW21_3365) hpf 3751096..3751455 (+) 360 WP_006024493.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  BW21_RS30255 (BW21_3366) ptsN 3751688..3752197 (+) 510 WP_004534308.1 PTS IIA-like nitrogen regulatory protein PtsN -
  BW21_RS30260 (BW21_3367) hprK 3752321..3753289 (+) 969 WP_004195225.1 HPr(Ser) kinase/phosphatase -
  BW21_RS30265 (BW21_3368) rapZ 3753366..3754259 (+) 894 WP_006024491.1 RNase adapter RapZ -
  BW21_RS30270 (BW21_3369) - 3754286..3754918 (+) 633 WP_009911186.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 55162.29 Da        Isoelectric Point: 4.8337

>NTDB_id=130128 BW21_RS30245 WP_006024494.1 3749438..3750946(+) (rpoN/rpoN1) [Burkholderia sp. 2002721687]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAIAQNPLLESDDDWIASPLRVAADGSVIAQTPAPSAPEPMA
GASNGTTATNERTERDEAAGVDEYDSYSADSGDANQWNLDDFGRSPAASDDDDLPPMQIHETSTSLRDHLMAQLRVTQAS
PRDRALVTFLIESLDDDGYLTASLEEVLADLPEELEVDVDELNAALALVHSFDPAGVGARSASECLKLQLLRLDPSPTRT
LALDIVSQHLELLAARDFTRLRKQLKATDDELRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKTGQNWQAELNPEVV
PKLRINHLYANILRNSRGDPSTGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTL
GLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVA
RRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=130128 BW21_RS30245 WP_006024494.1 3749438..3750946(+) (rpoN/rpoN1) [Burkholderia sp. 2002721687]
ATGAAAGCCAGTCTCCAACTTCGCCTGTCGCAGCATCTCGCGCTCACGCCCCAGCTTCAGCAGTCGATCCGGCTGCTGCA
GTTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGACGGCGATCGCGCAGAACCCGCTGCTCGAAAGCGACGACGACTGGA
TCGCGAGCCCGTTGCGCGTCGCGGCCGACGGCTCGGTGATCGCGCAGACGCCCGCGCCGTCCGCGCCCGAGCCGATGGCG
GGCGCGTCGAACGGCACGACTGCGACGAATGAGCGCACCGAGCGCGACGAAGCCGCCGGCGTCGACGAATACGACAGCTA
TTCGGCCGACAGCGGCGACGCGAACCAGTGGAACCTCGACGATTTCGGCCGCTCGCCCGCCGCGTCCGACGACGACGATC
TGCCGCCGATGCAGATCCACGAAACGTCGACGTCGCTGCGCGATCATCTGATGGCGCAGTTGCGCGTCACGCAGGCGAGC
CCGCGCGATCGCGCGCTCGTCACGTTCCTGATCGAATCGCTCGACGACGACGGCTATCTGACCGCGTCGCTCGAGGAAGT
GCTCGCCGATCTGCCCGAGGAGCTCGAAGTCGACGTCGACGAACTGAATGCGGCGCTCGCGCTCGTGCACAGCTTCGATC
CCGCGGGCGTCGGCGCGCGCTCCGCATCCGAATGCCTGAAGCTGCAACTGCTGCGCCTGGATCCGTCCCCGACGCGCACG
CTCGCCCTCGATATCGTGTCGCAGCATCTCGAGCTGCTCGCCGCGCGCGATTTCACGCGCCTGCGCAAGCAACTGAAGGC
GACCGACGACGAGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTCGAGCCGTTCCCCGGCGCGGCATACGGCAAGGCCG
AAGCCGATTACGTGGTGCCCGACATCATCGTGAAGAAGACCGGCCAGAACTGGCAGGCGGAACTCAATCCGGAAGTGGTG
CCGAAGCTGCGGATCAACCATCTTTACGCGAACATCCTGCGCAACAGCCGGGGCGATCCGAGCACGGGATCGCTGAAGCA
GCAGCTTCAGGAAGCGCGCTGGCTGATCAAGAATATTCAGCAGCGATTCGAGACGATCCTGCGCGTCGCGCAGGCGATTG
TCGAACGTCAGAAGAATTTCTTTGCGCACGGCGAAATCGCGATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTG
GGCCTACACGAATCGACTGTCTCCCGTGTGACAACCGGCAAGTACATGCTGACCCCGTTCGGCACGCTTGAATTTAAGTA
CTTCTTCGGATCGCACGTATCGACAGACACCGGAGGCGCCGCGTCGTCGACAGCGATCCGCGCCCTCATCAAACAACTGA
TAGGAGCCGAAGACCCCAAATCCCCTCTTTCGGACAGTCGCATAGCCGAGCTGCTAGCGGAACAAGGGTTCGTGGTCGCG
CGCCGCACGGTTGCCAAATATCGCGAAGCCCTCAAGATCCCGGCGGTGAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(345-500)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(138-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.762

100

0.697

Multiple sequence alignment