Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LACWKB8_RS06490 Genome accession   NZ_CP009531
Coordinates   1375746..1377122 (-) Length   458 a.a.
NCBI ID   WP_038523600.1    Uniprot ID   -
Organism   Lactobacillus sp. wkB8     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1370746..1382122
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LACWKB8_RS06465 (LACWKB8_1332) - 1371321..1371764 (-) 444 WP_038523590.1 Mini-ribonuclease 3 -
  LACWKB8_RS09405 - 1371832..1371939 (-) 108 WP_371211459.1 hypothetical protein -
  LACWKB8_RS06470 (LACWKB8_1333) - 1371939..1372184 (-) 246 WP_371211444.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family -
  LACWKB8_RS06475 (LACWKB8_1334) - 1372181..1372477 (-) 297 WP_038523591.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
  LACWKB8_RS06480 (LACWKB8_1335) cysS 1372582..1374006 (-) 1425 WP_038523594.1 cysteine--tRNA ligase -
  LACWKB8_RS06485 (LACWKB8_1336) gltX 1374172..1375671 (-) 1500 WP_038523597.1 glutamate--tRNA ligase -
  LACWKB8_RS06490 (LACWKB8_1337) radA 1375746..1377122 (-) 1377 WP_038523600.1 DNA repair protein RadA Machinery gene
  LACWKB8_RS06495 (LACWKB8_1338) - 1377123..1377674 (-) 552 WP_038523602.1 dUTP diphosphatase -
  LACWKB8_RS06500 (LACWKB8_1339) - 1377789..1378103 (+) 315 WP_038523604.1 hypothetical protein -
  LACWKB8_RS06505 (LACWKB8_1340) pepC 1378233..1379579 (+) 1347 WP_038523607.1 aminopeptidase C -
  LACWKB8_RS06510 (LACWKB8_1341) galT 1379674..1381152 (-) 1479 WP_038523610.1 UDP-glucose--hexose-1-phosphate uridylyltransferase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49974.77 Da        Isoelectric Point: 7.3471

>NTDB_id=129876 LACWKB8_RS06490 WP_038523600.1 1375746..1377122(-) (radA) [Lactobacillus sp. wkB8]
MAKLKTKYKCRSCGYISASYLGRCPNCGAWNQFEKETEAVQNRSTKGSPSRLIKKTGISEPVKLGNVKAEKEERIKTSMA
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLQIMSELADQYKVLYVSGEESANQIKLRADRLGLPANDMMLYPETDMEDI
RQQIADLEPDFVVIDSIQTMNEPSLDSMTGSASQVREVTSELMKIAKMDAITVFVIGHVTKEGAIAGPKILEHMVDTVLY
FEGDEHHAYRILHSVKNRFGAANEIGMFEMVNKGLQEVTNPSAVFLDERLPQSTGSAIVVSLEGTRPILAEIQALVTPTA
FGYAKRTTSGIDYNRAGLLLAVLEKRGNLMLQNQDVYLTATGGIRLNEPAVDLAVVMAIASSYKNQEILPTDCFVGEVGL
TGEVRRVNQIDARIKEAVKTGFKRIFIPKHNMRASLKNSGIEVIPVASIPQALKVVLG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=129876 LACWKB8_RS06490 WP_038523600.1 1375746..1377122(-) (radA) [Lactobacillus sp. wkB8]
ATGGCAAAACTGAAAACAAAATATAAATGCCGTTCTTGTGGTTATATCTCTGCCAGCTATTTAGGACGCTGTCCTAATTG
TGGTGCTTGGAATCAGTTTGAAAAAGAAACTGAAGCAGTGCAAAACCGTTCAACTAAAGGCAGTCCCAGCAGGCTCATCA
AGAAAACCGGAATTAGCGAACCGGTTAAATTGGGGAATGTGAAGGCTGAAAAAGAAGAGCGCATCAAAACAAGTATGGCG
GAGCTGAACAGGGTTTTAGGCGGCGGTATTGTTCCCGGTTCACTGGTTTTAATCGGTGGTGACCCTGGAATCGGTAAGTC
AACTTTAATGTTACAAATCATGAGCGAATTGGCTGATCAATATAAAGTGCTGTATGTTTCAGGGGAAGAATCTGCTAATC
AAATCAAGCTCCGTGCAGATCGCTTAGGTCTTCCTGCTAATGACATGATGCTTTATCCAGAAACAGATATGGAAGATATT
CGTCAGCAAATTGCTGATTTAGAGCCCGATTTTGTCGTGATTGACTCCATTCAGACCATGAATGAACCCAGTCTTGACTC
GATGACCGGTTCGGCGTCACAAGTAAGGGAAGTCACAAGCGAATTGATGAAAATCGCTAAAATGGATGCGATTACCGTTT
TTGTTATTGGTCACGTGACAAAAGAAGGTGCGATTGCAGGTCCTAAAATTCTGGAGCACATGGTAGATACAGTCCTTTAT
TTTGAAGGTGATGAACATCATGCCTACCGTATTTTACATTCTGTTAAAAATCGGTTTGGTGCTGCAAATGAAATCGGCAT
GTTTGAAATGGTTAACAAAGGTTTGCAAGAAGTAACTAACCCTTCAGCCGTATTCTTAGATGAACGGTTGCCCCAATCTA
CCGGTTCGGCGATAGTCGTATCGCTTGAAGGAACAAGGCCAATTTTGGCGGAAATTCAGGCATTAGTGACACCGACTGCT
TTTGGTTATGCAAAAAGAACCACTTCGGGGATTGACTACAATAGAGCTGGACTATTGCTAGCTGTCTTGGAAAAAAGAGG
CAACTTGATGCTGCAAAATCAAGACGTTTATCTAACTGCCACTGGAGGAATTCGTTTGAACGAACCGGCAGTTGATTTGG
CAGTTGTCATGGCAATTGCCTCAAGTTATAAAAATCAGGAAATTTTGCCTACAGACTGTTTTGTGGGCGAAGTCGGCTTA
ACTGGTGAGGTGCGCAGAGTCAATCAAATAGATGCGCGTATTAAAGAAGCTGTTAAGACTGGTTTCAAACGTATTTTTAT
TCCCAAACATAATATGCGTGCTAGTCTTAAAAACTCGGGAATTGAAGTGATTCCAGTAGCAAGTATTCCGCAGGCCTTAA
AAGTGGTTTTAGGGTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(68-223)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-34)

Subunit ChlI of Mg-chelatase (ChlI)

(364-431)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

58.297

100

0.583
  radA Streptococcus pneumoniae Rx1

58.297

100

0.583
  radA Streptococcus pneumoniae D39

58.297

100

0.583
  radA Streptococcus pneumoniae R6

58.297

100

0.583
  radA Streptococcus pneumoniae TIGR4

58.297

100

0.583
  radA Streptococcus mitis SK321

58.297

100

0.583
  radA Bacillus subtilis subsp. subtilis str. 168

56.399

100

0.568

Multiple sequence alignment