Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACJ74_RS24610 Genome accession   NZ_CP011915
Coordinates   4810525..4810965 (+) Length   146 a.a.
NCBI ID   WP_000360913.1    Uniprot ID   -
Organism   Escherichia coli strain PSUO2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4805525..4815965
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJ74_RS24585 (ACJ74_23670) aroP 4806525..4807895 (+) 1371 WP_000399017.1 aromatic amino acid transporter AroP -
  ACJ74_RS24590 (ACJ74_23675) ampE 4807938..4808792 (-) 855 WP_000171984.1 beta-lactamase regulator AmpE -
  ACJ74_RS24595 (ACJ74_23680) ampD 4808789..4809340 (-) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACJ74_RS24600 (ACJ74_23685) nadC 4809428..4810321 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACJ74_RS24610 (ACJ74_23695) pilA 4810525..4810965 (+) 441 WP_000360913.1 prepilin peptidase-dependent pilin Machinery gene
  ACJ74_RS24615 (ACJ74_23700) pilB 4810975..4812360 (+) 1386 WP_001025178.1 type II secretion system protein GspE Machinery gene
  ACJ74_RS24620 (ACJ74_23705) hofC 4812350..4813552 (+) 1203 WP_000157292.1 protein transport protein HofC -
  ACJ74_RS24625 (ACJ74_23710) guaC 4813587..4814630 (-) 1044 WP_001217343.1 GMP reductase -
  ACJ74_RS26355 - 4814786..4814830 (-) 45 WP_120795372.1 protein YacM -
  ACJ74_RS24630 (ACJ74_23715) coaE 4814855..4815475 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15634.80 Da        Isoelectric Point: 4.5237

>NTDB_id=129857 ACJ74_RS24610 WP_000360913.1 4810525..4810965(+) (pilA) [Escherichia coli strain PSUO2]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSIVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=129857 ACJ74_RS24610 WP_000360913.1 4810525..4810965(+) (pilA) [Escherichia coli strain PSUO2]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCATCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363