Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ACJ74_RS06345 Genome accession   NZ_CP011915
Coordinates   1276294..1276971 (-) Length   225 a.a.
NCBI ID   WP_000178166.1    Uniprot ID   -
Organism   Escherichia coli strain PSUO2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1271294..1281971
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJ74_RS06330 (ACJ74_06130) chiA 1272652..1275354 (+) 2703 WP_000773168.1 bifunctional chitinase/lysozyme -
  ACJ74_RS06335 (ACJ74_06135) bfd 1275523..1275717 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  ACJ74_RS06340 (ACJ74_06140) bfr 1275789..1276265 (+) 477 WP_000675504.1 bacterioferritin -
  ACJ74_RS06345 (ACJ74_06145) pilD 1276294..1276971 (-) 678 WP_000178166.1 prepilin peptidase GspO Machinery gene
  ACJ74_RS06350 (ACJ74_06150) gspM 1276971..1277432 (-) 462 WP_001298207.1 type II secretion system protein GspM -
  ACJ74_RS06355 (ACJ74_06155) gspL 1277429..1278592 (-) 1164 WP_088895517.1 type II secretion system protein GspL -
  ACJ74_RS06360 (ACJ74_06160) gspK 1278607..1279590 (-) 984 WP_001058508.1 type II secretion system minor pseudopilin GspK -
  ACJ74_RS06365 (ACJ74_06165) gspJ 1279583..1280170 (-) 588 WP_000610638.1 type II secretion system minor pseudopilin GspJ -
  ACJ74_RS06370 (ACJ74_06170) gspI 1280163..1280540 (-) 378 WP_001041750.1 type II secretion system minor pseudopilin GspI -
  ACJ74_RS06375 (ACJ74_06175) gspH 1280537..1281046 (-) 510 WP_001076040.1 type II secretion system minor pseudopilin GspH -
  ACJ74_RS06380 (ACJ74_06180) gspG 1281054..1281491 (-) 438 WP_001202878.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24814.64 Da        Isoelectric Point: 7.6364

>NTDB_id=129807 ACJ74_RS06345 WP_000178166.1 1276294..1276971(-) (pilD) [Escherichia coli strain PSUO2]
MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLAHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=129807 ACJ74_RS06345 WP_000178166.1 1276294..1276971(-) (pilD) [Escherichia coli strain PSUO2]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGCTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.382

Multiple sequence alignment