Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   ACJ74_RS02995 Genome accession   NZ_CP011915
Coordinates   601208..603406 (+) Length   732 a.a.
NCBI ID   WP_001340148.1    Uniprot ID   B7UNQ5
Organism   Escherichia coli strain PSUO2     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 596208..608406
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJ74_RS02975 (ACJ74_02855) metB 598310..599470 (-) 1161 WP_000197201.1 cystathionine gamma-synthase -
  ACJ74_RS02980 (ACJ74_02860) metJ 599747..600064 (+) 318 WP_000852812.1 met regulon transcriptional regulator MetJ -
  ACJ74_RS02985 (ACJ74_02865) yiiX 600124..600732 (+) 609 WP_000702302.1 YiiX family permuted papain-like enzyme -
  ACJ74_RS02990 (ACJ74_02870) rpmE 600793..601005 (-) 213 WP_000710769.1 50S ribosomal protein L31 -
  ACJ74_RS02995 (ACJ74_02875) priA 601208..603406 (+) 2199 WP_001340148.1 primosomal protein N' Machinery gene
  ACJ74_RS03000 (ACJ74_02880) cytR 603562..604587 (+) 1026 WP_000644904.1 DNA-binding transcriptional regulator CytR Regulator
  ACJ74_RS03005 (ACJ74_02885) ftsN 604679..605638 (+) 960 WP_000068847.1 cell division protein FtsN -
  ACJ74_RS03010 (ACJ74_02890) hslV 605731..606261 (+) 531 WP_000208242.1 ATP-dependent protease subunit HslV -
  ACJ74_RS03015 (ACJ74_02895) hslU 606271..607602 (+) 1332 WP_001293344.1 HslU--HslV peptidase ATPase subunit -

Sequence

Protein


Download         Length: 732 a.a.        Molecular weight: 81638.94 Da        Isoelectric Point: 9.2478

>NTDB_id=129784 ACJ74_RS02995 WP_001340148.1 601208..603406(+) (priA) [Escherichia coli strain PSUO2]
MPVAHVALPVPLPRTFDYLLPEGMTVKAGSRVRVPFGKQQERIGVVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRL
LLWAADYYHHPIGDVLFHALPILLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFSAWLLAGVTGSGKTEVYLSVLEN
VLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVID
EEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVLDLKGQKVQ
AGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQQHLRCHHCDSQRPVPRQCPSCG
STHLVPVGLGTEQLEQTLAPLFPGVPISRIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVD
GALFSADFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAERRMMQLPPWTSHVIVR
AEDHNNQHAPLFLQQLRNLILASPLADEKLWVLGPVPALAPKRGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRK
VKWVLDVDPIEG

Nucleotide


Download         Length: 2199 bp        

>NTDB_id=129784 ACJ74_RS02995 WP_001340148.1 601208..603406(+) (priA) [Escherichia coli strain PSUO2]
ATGCCCGTTGCCCACGTTGCCTTGCCCGTTCCGCTTCCTCGTACCTTTGACTATCTGCTGCCAGAAGGCATGACGGTTAA
AGCCGGGAGTCGCGTGCGCGTGCCGTTTGGCAAACAACAGGAGCGTATCGGTGTTGTGGTGTCAGTGAGCGATGTCAGTG
AGCTGCCGCTTAACGAGCTGAAAGCGGTAGTTGAAGTGCTGGACGTTGAGCCGGTATTTACTCACTCTGTCTGGCGATTA
CTGCTCTGGGCGGCAGATTACTATCATCATCCGATTGGCGATGTGCTGTTTCATGCCTTGCCGATTTTACTGCGCCAGGG
GCGGCCTGCGGCGAACGCGCCGATGTGGTACTGGTTTGCCACTGAACAAGGTCAGGCGGTGGATCTGAACAGCCTGAAAC
GTTCGCCAAAGCAACAACAGGCGCTGGCAGCGTTACGGCAAGGCAAAATCTGGCGCGACCAGGTTGCCACGCTCGAATTT
AATGATGCCGCGTTGCAGGCACTGCGCAAAAAAGGTCTGTGTGATTTAGCAAGTGAAACACCAGAGTTTAGCGACTGGCG
AACGAACTATGCCGTTTCTGGTGAGCGGTTGCGGTTGAATACCGAACAGGCCACCGCCGTTGGCGCAATTCATAGCGCGG
CAGATACTTTTTCTGCCTGGCTGCTGGCGGGCGTTACCGGTTCCGGTAAAACGGAGGTTTATCTCAGTGTACTGGAAAAC
GTGCTCGCTCAGGGCAAACAGGCGCTGGTGATGGTGCCGGAAATCGGCCTGACACCGCAAACTATCGCCCGTTTTCGTGA
ACGTTTTAATGCCCCGGTGGAAGTTCTGCATTCCGGCCTGAACGACAGCGAGCGTCTTTCGGCGTGGTTGAAAGCGAAAA
ATGGCGAGGCGGCGATTGTCATCGGCACCCGCTCCGCGCTGTTTACGCCGTTTAAAAATCTCGGCGTGATTGTTATTGAT
GAAGAGCACGACAGCTCCTACAAGCAGCAGGAAGGCTGGCGCTATCATGCCCGCGACCTGGCGGTGTATCGCGCGCATAG
CGAGCAAATCCCAATTATTCTTGGCTCCGCAACGCCCGCACTGGAAACGTTATGCAACGTGCAGCAGAAAAAATACCGCC
TGCTGCGCCTGACCCGTCGGGCGGGCAATGCGCGCCCGGCAATTCAACATGTGCTGGATTTAAAAGGTCAGAAGGTGCAG
GCAGGTCTGGCTCCGGCGTTAATCACCCGTATGCGCCAGCATTTACAGGCCAACAATCAGGTTATCCTCTTTCTTAATCG
TCGGGGTTTTGCGCCTGCGCTGCTGTGCCACGACTGCGGCTGGATAGCCGAATGCCCACGTTGCGATCACTACTACACGC
TGCATCAGGCGCAACAGCATTTGCGCTGCCACCATTGCGACAGTCAGCGTCCGGTGCCGCGCCAGTGCCCTTCTTGCGGT
TCCACGCACCTGGTACCCGTGGGGCTGGGCACCGAACAGCTTGAACAGACGCTCGCGCCGTTGTTCCCTGGTGTCCCCAT
TTCTCGTATCGACCGCGATACCACCAGCCGCAAAGGGGCGCTGGAACAGCAACTGGCGGAAGTGCATCGCGGCGGCGCGA
GGATCTTGATTGGCACGCAAATGCTGGCGAAAGGTCACCACTTTCCGGATGTGACGCTGGTGGCGTTACTGGATGTGGAC
GGCGCGCTGTTTTCTGCCGATTTTCGCTCGGCAGAACGTTTCGCCCAGCTTTACACCCAGGTCGCCGGTCGTGCCGGGCG
TGCGGGTAAACAGGGCGAAGTGGTGCTGCAAACGCACCATCCGGAACATCCTCTGTTGCAAACATTGCTCTATAAAGGCT
ACGACGCCTTTGCCGAACAGGCACTGGCTGAACGACGCATGATGCAGCTGCCGCCGTGGACCAGCCATGTGATTGTGCGT
GCGGAAGATCATAACAATCAGCACGCGCCATTGTTCCTGCAACAACTGCGTAATCTCATTCTCGCCAGCCCACTGGCAGA
CGAGAAACTGTGGGTTCTCGGTCCGGTTCCGGCGCTGGCCCCTAAACGTGGCGGTCGCTGGCGCTGGCAGATATTGTTGC
AGCACCCTTCCCGCGTGCGCTTACAGCACATCATCAATGGTACGCTGGCGCTTATTAATACAATACCGGATTCCCGCAAG
GTGAAATGGGTGCTGGATGTCGATCCGATTGAGGGTTAA

Domains

Predicted by InterProScan.

Primosomal protein N C-terminal domain (PriA_C)

(632-729)

DEAD/DEAH box helicase (DEAD)

(203-362)

3DNA-binding domain (3BD) (PriA_3primeBD)

(6-101)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(445-471)

Helicase conserved C-terminal domain (Helicase_C)

(522-589)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7UNQ5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

45.047

100

0.454

Multiple sequence alignment