Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BW23_RS25415 Genome accession   NZ_CP009488
Coordinates   2252493..2253998 (-) Length   501 a.a.
NCBI ID   WP_042586339.1    Uniprot ID   -
Organism   Burkholderia ubonensis MSMB22     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2247493..2258998
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BW23_RS25390 (BW23_2089) - 2248508..2249146 (-) 639 WP_042586341.1 LON peptidase substrate-binding domain-containing protein -
  BW23_RS25395 (BW23_2090) rapZ 2249173..2250081 (-) 909 WP_042586340.1 RNase adapter RapZ -
  BW23_RS25400 (BW23_2091) hprK 2250158..2251126 (-) 969 WP_010090664.1 HPr(Ser) kinase/phosphatase -
  BW23_RS25405 (BW23_2092) - 2251263..2251775 (-) 513 WP_029226338.1 PTS sugar transporter subunit IIA -
  BW23_RS25410 (BW23_2093) hpf 2251966..2252334 (-) 369 WP_010090666.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  BW23_RS25415 (BW23_2094) rpoN/rpoN1 2252493..2253998 (-) 1506 WP_042586339.1 RNA polymerase factor sigma-54 Machinery gene
  BW23_RS25420 (BW23_2095) lptB 2254165..2254941 (-) 777 WP_010090668.1 LPS export ABC transporter ATP-binding protein -
  BW23_RS25425 (BW23_2096) lptA 2254938..2255603 (-) 666 WP_045567160.1 lipopolysaccharide transport periplasmic protein LptA -
  BW23_RS25430 (BW23_2097) lptC 2255624..2256238 (-) 615 WP_045567161.1 LPS export ABC transporter periplasmic protein LptC -
  BW23_RS25435 (BW23_2098) - 2256242..2256778 (-) 537 WP_042586336.1 KdsC family phosphatase -
  BW23_RS25440 (BW23_2099) kdsD 2256778..2257761 (-) 984 WP_042586335.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54782.00 Da        Isoelectric Point: 4.8190

>NTDB_id=129764 BW23_RS25415 WP_042586339.1 2252493..2253998(-) (rpoN/rpoN1) [Burkholderia ubonensis MSMB22]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLESDDEWIASPLRVAADGSLIAQTPPASNAEPMA
ATNGAGQTGERTERDEPAAADDYDGYASGDSDGPQWNLDEFGRASGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEVDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQYLELLAARDFTRLRKHLKANDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKVGQGWQAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDTKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=129764 BW23_RS25415 WP_042586339.1 2252493..2253998(-) (rpoN/rpoN1) [Burkholderia ubonensis MSMB22]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACACCTGGCGCTGACGCCGCAACTGCAGCAGTCGATCCGGCTCCTGCA
GCTGTCGACGCTCGAACTGCAGCAGGAAGTGGCGATGGCGGTCGCCCAGAATCCGCTGCTCGAGAGCGACGATGAATGGA
TCGCGAGCCCGCTGCGCGTTGCCGCGGACGGTTCGCTGATCGCGCAGACGCCGCCCGCGTCGAACGCCGAGCCGATGGCG
GCCACGAACGGCGCCGGCCAGACCGGCGAGCGCACCGAACGCGACGAGCCTGCCGCGGCCGACGACTACGACGGCTACGC
GTCCGGCGACAGCGACGGCCCGCAATGGAACCTCGACGAATTCGGCCGCGCGAGCGGCGCGTCGGATGACGACGACCTGC
CGCCGCTGCAGGTCCAGGAAGCGGCGACGTCGCTGCGCGACCACCTGTCCGCGCAGCTGCGCGTCACGCAGGCCGGGCCG
CGCGACCGTGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGACGAGGTGCT
CGCCGACCTGCCGCCCGAACTCGAAGTCGATTGCGACGAACTGAACGCCGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CCGGGGTCGGGGCCCGCTCGGCGTCCGAGTGCCTGAAGCTGCAATTGCTGCGGCTCGATCCGTCCCCGACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAATATCTCGAGCTGCTCGCCGCGCGCGACTTCACGCGGTTGCGCAAGCACCTGAAGGCGAA
CGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCGTACGGCAAGGCCGAAG
CGGACTACGTCGTGCCCGACATCATCGTGAAGAAGGTCGGCCAGGGCTGGCAGGCGGAGCTGAATCCCGAAGTCGTGCCG
CGCCTGCGGATCAACAATCTCTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCCGCGGGTTCGCTCAAGCAGCA
GCTGCAGGAAGCGCGCTGGCTGATCAAGAATATCCAGCAGCGGTTCGACACGATCCTGCGAGTTGCGCAGGCTATTGTCG
AACGTCAGAAGAATTTCTTTGCGCACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTCTCGACCGACACCGGTGGCGCCGCATCGTCGACCGCCATCCGTGCCCTGATCAAGCAACTGATAG
GAGCCGAAGACACCAAATCGCCTCTTTCGGACAGCCGCATTGCCGAACTGCTGGCAGAACAAGGCTTCGTGGTCGCGCGC
CGCACGGTTGCCAAGTATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.119

100

0.687

Multiple sequence alignment