Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AB831_RS02100 Genome accession   NZ_CP011884
Coordinates   417612..419336 (+) Length   574 a.a.
NCBI ID   WP_005456306.1    Uniprot ID   Q87LB1
Organism   Vibrio parahaemolyticus strain MAVP-Q     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 412612..424336
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB831_RS02075 (AB831_01945) gspM 413017..413667 (+) 651 WP_005497276.1 type II secretion system protein GspM -
  AB831_RS02080 (AB831_01950) - 413660..413989 (+) 330 WP_005481006.1 MSHA biogenesis protein MshK -
  AB831_RS02085 (AB831_01955) mshL 414010..415653 (+) 1644 WP_005456314.1 pilus (MSHA type) biogenesis protein MshL -
  AB831_RS02090 (AB831_01960) - 415697..416542 (+) 846 WP_005497278.1 ExeA family protein -
  AB831_RS02095 (AB831_01965) - 416539..417615 (+) 1077 WP_021450080.1 tetratricopeptide repeat protein -
  AB831_RS02100 (AB831_01970) pilB 417612..419336 (+) 1725 WP_005456306.1 GspE/PulE family protein Machinery gene
  AB831_RS02105 (AB831_01975) - 419345..420568 (+) 1224 WP_021450079.1 type II secretion system F family protein -
  AB831_RS02110 (AB831_01980) - 420572..421033 (+) 462 WP_020835335.1 hypothetical protein -
  AB831_RS25705 - 421130..421279 (+) 150 WP_021450078.1 hypothetical protein -
  AB831_RS02115 (AB831_01985) - 421327..421782 (+) 456 WP_021450077.1 type II secretion system protein -
  AB831_RS02120 (AB831_01990) - 421854..422087 (+) 234 WP_005481019.1 hypothetical protein -
  AB831_RS02125 (AB831_01995) - 422203..422688 (+) 486 WP_021450076.1 type II secretion system protein -
  AB831_RS02130 (AB831_02000) - 422685..423254 (+) 570 WP_021450075.1 type IV pilus modification PilV family protein -
  AB831_RS02135 (AB831_02005) - 423254..424003 (+) 750 WP_021450074.1 PilW family protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63886.21 Da        Isoelectric Point: 5.2324

>NTDB_id=129702 AB831_RS02100 WP_005456306.1 417612..419336(+) (pilB) [Vibrio parahaemolyticus strain MAVP-Q]
MKIQLRKRLGDLLVEEGIVSEDQIQQALSAQRSTGQKLGDALIDLGFITEKQMLEFLSQQLGLPLIDLGRAPVDADAVQI
LPEVHARRLRAMVVARNGDTLRVAMSDPADLFTQESLMNLLGEYNLEFIIASERQLISSFDRYYRRTKEIASFAEQLQAE
HQDVQSFDYGIDEADSEEVTVVKLVNSMFEDAVQVGASDIHIEPDDKVLRLRQRVDGVLHETILNEVNIASALVLRLKLM
AHLDISEKRLPQDGRFNIKVRGQSIDIRMSTLPTQYGESVVMRLLNQSSGLRPLEESGLPPELLARLRRQLSRPHGMILV
TGPTGSGKTTTLYGALSELNEPGKKIITAEDPVEYRLPRITQVQINSKIDLTFSRVLRTFLRQDPDIILIGEMRDQETVE
IGLRAALTGHLVLSTLHTNDAVDSALRMIDMGAPGYLVASAVRAVVAQRLVRRVCPDCKTQDHLDESRQQWLAGRFPNQV
GVTFHKGAGCQNCNLTGYRGRIGVFEMLELEHEMMDKLRANDAVGFAQAARRSENYKPLLASAMELALQGAVSLDEVMTL
GEGDASGKTDPIFM

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=129702 AB831_RS02100 WP_005456306.1 417612..419336(+) (pilB) [Vibrio parahaemolyticus strain MAVP-Q]
ATGAAAATTCAGTTGAGAAAACGCTTAGGTGACCTACTCGTTGAAGAAGGCATCGTATCGGAAGATCAAATCCAGCAAGC
TCTATCAGCTCAGCGCAGTACCGGACAAAAATTAGGTGATGCCCTGATTGACCTCGGGTTTATTACCGAAAAGCAAATGC
TGGAGTTTTTGTCTCAGCAACTAGGATTACCGTTGATCGATCTTGGACGTGCTCCAGTGGATGCGGATGCGGTGCAAATA
CTGCCAGAAGTGCATGCTCGTCGTTTACGTGCAATGGTGGTGGCTCGTAATGGTGACACTTTACGTGTTGCGATGAGTGA
TCCGGCGGATCTGTTTACCCAAGAGTCTTTAATGAACTTGCTAGGTGAGTACAACCTTGAGTTCATCATAGCCTCTGAGC
GTCAGTTGATCAGCAGTTTCGACCGTTACTACCGCCGTACCAAAGAGATCGCGTCCTTTGCTGAGCAGTTACAAGCCGAG
CACCAAGACGTACAAAGCTTTGACTATGGCATCGATGAAGCTGACAGTGAAGAAGTCACCGTTGTAAAACTGGTTAATTC
GATGTTTGAAGACGCGGTGCAAGTTGGTGCATCGGATATCCATATCGAACCAGACGATAAAGTCCTGCGTTTACGCCAGC
GTGTCGATGGTGTGCTGCATGAAACCATTCTGAACGAAGTGAACATCGCTTCTGCATTGGTATTACGCCTTAAATTGATG
GCGCACTTGGATATTTCTGAGAAGCGTCTGCCGCAAGATGGTCGCTTCAACATCAAAGTTCGTGGTCAATCGATTGATAT
TCGTATGTCGACGTTACCGACTCAGTACGGTGAATCGGTCGTAATGCGTCTGCTTAACCAGTCGTCAGGTTTACGTCCAT
TAGAAGAATCGGGTTTACCGCCTGAGCTACTGGCGCGCTTGCGTCGTCAGTTATCTCGTCCTCATGGCATGATTTTGGTA
ACCGGCCCTACGGGGTCGGGTAAAACCACGACACTTTACGGCGCGTTGAGTGAGCTCAATGAACCGGGTAAAAAAATCAT
CACCGCAGAAGATCCGGTGGAATACCGTTTACCACGTATCACGCAAGTACAGATCAACAGCAAAATCGACCTGACATTTT
CACGCGTGTTACGCACCTTCTTGCGTCAAGACCCAGACATCATTTTGATTGGTGAGATGCGTGACCAAGAGACGGTAGAG
ATTGGCTTGCGAGCAGCATTAACTGGTCACTTAGTATTGAGTACGTTGCACACCAACGATGCGGTCGACAGCGCACTGCG
TATGATCGACATGGGGGCGCCAGGCTATCTCGTCGCCAGTGCCGTGCGTGCCGTTGTGGCTCAGCGATTGGTTCGCCGTG
TTTGTCCAGATTGTAAAACCCAAGATCATCTCGATGAATCGCGGCAACAATGGTTAGCGGGGCGTTTTCCAAATCAAGTT
GGGGTGACTTTCCACAAAGGAGCAGGTTGTCAAAACTGTAACTTGACGGGGTATCGAGGTCGAATCGGTGTGTTTGAAAT
GCTTGAGCTGGAACATGAGATGATGGACAAGCTGCGCGCGAATGATGCGGTTGGCTTTGCACAAGCGGCTCGACGTTCAG
AAAACTACAAACCGCTTCTTGCCTCAGCAATGGAGCTTGCACTGCAAGGTGCTGTGAGCCTGGATGAAGTGATGACCCTC
GGAGAGGGTGACGCTTCGGGTAAAACTGATCCCATTTTCATGTAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-148)

Type II/IV secretion system protein (T2SSE)

(187-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87LB1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.819

97.387

0.378
  pilB/pilB1 Synechocystis sp. PCC 6803

34.272

100

0.361

Multiple sequence alignment