Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   FORC16_RS12805 Genome accession   NZ_CP011775
Coordinates   2810026..2811750 (-) Length   574 a.a.
NCBI ID   WP_011079397.1    Uniprot ID   A0A3Q0L3L1
Organism   Vibrio vulnificus strain FORC_016     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2805026..2816750
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC16_RS12770 (FORC16_2516) - 2805366..2806055 (-) 690 WP_060530679.1 PilW family protein -
  FORC16_RS12775 (FORC16_2517) - 2806055..2806621 (-) 567 WP_060530678.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FORC16_RS12780 (FORC16_2518) - 2806621..2807118 (-) 498 WP_060530676.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FORC16_RS12785 (FORC16_2519) - 2807170..2807640 (-) 471 WP_045596380.1 type II secretion system protein -
  FORC16_RS12790 (FORC16_2520) - 2807674..2808252 (-) 579 WP_011079400.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FORC16_RS12795 (FORC16_2521) - 2808331..2808792 (-) 462 WP_039554781.1 hypothetical protein -
  FORC16_RS12800 (FORC16_2522) - 2808796..2810016 (-) 1221 WP_045620604.1 type II secretion system F family protein -
  FORC16_RS12805 (FORC16_2523) pilF 2810026..2811750 (-) 1725 WP_011079397.1 GspE/PulE family protein Machinery gene
  FORC16_RS12810 (FORC16_2524) - 2811747..2812868 (-) 1122 WP_060530674.1 tetratricopeptide repeat protein -
  FORC16_RS12815 (FORC16_2525) - 2812865..2813713 (-) 849 WP_060530672.1 ExeA family protein -
  FORC16_RS12820 (FORC16_2526) mshL 2813721..2815367 (-) 1647 WP_072605704.1 pilus (MSHA type) biogenesis protein MshL -
  FORC16_RS12825 (FORC16_2527) - 2815383..2815703 (-) 321 WP_011079393.1 MSHA biogenesis protein MshK -
  FORC16_RS12830 (FORC16_2528) gspM 2815696..2816346 (-) 651 WP_011079392.1 type II secretion system protein GspM -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63672.03 Da        Isoelectric Point: 5.3226

>NTDB_id=129362 FORC16_RS12805 WP_011079397.1 2810026..2811750(-) (pilF) [Vibrio vulnificus strain FORC_016]
MKVRLRKRLGDLLVEEGIINEAQLDQALGAQRSGGRKLGDTLIDLGFLSEQQMLAFLSQQLDLPLIDLNRATVDIDAVQL
LPEVHARRLRALVIGRNGDTLRIAMSDPADLFAQEALIGQLGQYGIEFVIAPERQLVEGFDRYYRRTKEIANFAEQLQAE
HQVNDAFDFNIEDDDSDEVTVVKLINSMFEDAIQVGASDIHIEPDANVLRLRQRIDGVLHETLLNEVNIAPALVLRLKLM
ANLDISEKRLPQDGRFNIRSKGQSVDIRMSTMPVQYGESVVMRLLNQSSGVRKLEDSGLPDDLLVRLRRQLKRPHGMILV
TGPTGSGKTTTLYGALTELNQPGKKIITAEDPVEYRLSRVNQVQINPKINLDFSTVLRTFLRQDPDIILIGEMRDHETVE
IGLRAALTGHLVLSTLHTNDAVDSALRMIDMGAPGYLVASAVRAVVAQRLVRRVCPDCKTEDHVGESRKAWLAQRFPNQV
EAGFVKGRGCQNCNLTGYRGRIGVFEMLELEHGMMDALRANDAVEFAHAARRSPGYKPLLASAMELALQGVVSLDEVMNL
GEGDSSGVAEAIIM

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=129362 FORC16_RS12805 WP_011079397.1 2810026..2811750(-) (pilF) [Vibrio vulnificus strain FORC_016]
ATGAAAGTAAGATTAAGAAAGCGACTTGGTGACCTTCTTGTTGAGGAAGGCATTATCAATGAAGCGCAACTTGATCAAGC
ACTCGGCGCGCAAAGAAGTGGTGGACGCAAGCTTGGCGATACGCTGATTGATTTGGGTTTTTTGTCTGAACAACAGATGC
TGGCGTTTTTGTCTCAGCAACTTGATCTGCCTTTGATTGACTTAAATCGAGCCACGGTCGATATCGATGCAGTGCAGTTG
TTACCAGAAGTGCATGCTCGTCGTTTACGCGCATTGGTGATTGGCCGTAATGGCGACACGTTGCGCATTGCGATGAGTGA
TCCAGCGGACCTTTTTGCGCAAGAAGCGTTGATCGGTCAGCTAGGACAATATGGCATTGAGTTTGTCATCGCGCCAGAAA
GGCAATTGGTGGAAGGCTTTGATCGCTATTATCGCCGTACCAAAGAGATCGCCAACTTTGCTGAGCAGTTACAAGCGGAA
CACCAAGTCAACGATGCATTCGATTTCAATATCGAAGATGACGACAGCGATGAGGTGACGGTCGTTAAGCTCATCAACTC
GATGTTTGAAGACGCCATCCAGGTGGGCGCGTCGGATATCCATATCGAACCGGACGCCAATGTGTTGCGCTTGCGTCAAC
GTATTGATGGCGTGTTGCACGAAACCTTGCTCAATGAAGTCAACATCGCGCCAGCATTGGTACTGAGATTGAAACTCATG
GCCAACTTGGATATTTCAGAAAAGCGTCTGCCGCAAGATGGCCGTTTTAACATCCGATCCAAAGGTCAGTCGGTGGATAT
CCGTATGTCGACCATGCCAGTTCAGTATGGTGAGTCGGTGGTAATGCGTTTGCTCAATCAATCTTCCGGAGTCAGAAAAT
TAGAAGACTCCGGTTTGCCGGACGATCTTTTAGTGCGTCTGCGCCGACAGTTGAAACGTCCGCATGGCATGATCCTTGTT
ACTGGTCCTACGGGATCCGGTAAAACCACCACGCTATATGGTGCTCTAACAGAATTAAACCAACCGGGGAAAAAGATCAT
CACAGCGGAAGATCCGGTTGAATACCGACTGTCGCGTGTCAACCAAGTGCAGATCAATCCGAAGATCAACCTTGATTTTT
CCACCGTGTTAAGAACATTCCTCCGTCAGGATCCCGATATCATTTTGATTGGTGAGATGCGTGACCATGAAACGGTTGAA
ATTGGCCTGCGAGCGGCGCTGACCGGTCACTTAGTATTGAGTACCTTACACACCAATGACGCCGTAGACAGTGCGCTTCG
TATGATTGATATGGGTGCTCCGGGCTATCTGGTCGCGAGTGCCGTACGTGCTGTGGTCGCGCAGCGATTGGTGCGCCGTG
TCTGTCCGGATTGCAAGACAGAAGATCATGTTGGTGAGTCACGCAAAGCGTGGTTGGCACAACGCTTCCCGAATCAAGTG
GAAGCAGGCTTTGTGAAAGGGCGCGGTTGTCAAAACTGTAACTTAACAGGTTATCGCGGCCGTATTGGTGTATTTGAAAT
GCTGGAACTTGAGCATGGAATGATGGACGCGTTGCGCGCCAATGATGCCGTCGAGTTTGCACATGCTGCGCGTCGTTCGC
CTGGCTATAAACCTCTGTTAGCGTCGGCAATGGAGTTGGCGCTGCAAGGCGTAGTGAGCTTAGACGAAGTGATGAATTTA
GGCGAAGGTGACTCCTCTGGGGTTGCTGAAGCGATCATTATGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-148)

Type II/IV secretion system protein (T2SSE)

(187-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q0L3L1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

38.104

97.387

0.371
  pilB Vibrio cholerae strain A1552

37.699

98.432

0.371