Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   LG71_RS01205 Genome accession   NZ_CP009450
Coordinates   256538..257971 (+) Length   477 a.a.
NCBI ID   WP_043086293.1    Uniprot ID   A0A089PID0
Organism   Pluralibacter gergoviae strain FB2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 251538..262971
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG71_RS01175 (LG71_01190) - 252093..253070 (+) 978 WP_043080888.1 calcium/sodium antiporter -
  LG71_RS01180 (LG71_01195) kdsD 253085..254071 (+) 987 WP_043080889.1 arabinose-5-phosphate isomerase KdsD -
  LG71_RS01185 (LG71_01200) kdsC 254088..254654 (+) 567 WP_043080890.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  LG71_RS01190 (LG71_01205) lptC 254651..255223 (+) 573 WP_043080891.1 LPS export ABC transporter periplasmic protein LptC -
  LG71_RS01195 (LG71_01210) lptA 255204..255758 (+) 555 WP_043080892.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  LG71_RS01200 (LG71_01215) lptB 255765..256490 (+) 726 WP_043080893.1 LPS export ABC transporter ATP-binding protein -
  LG71_RS01205 (LG71_01220) rpoN/rpoN1 256538..257971 (+) 1434 WP_043086293.1 RNA polymerase factor sigma-54 Machinery gene
  LG71_RS01210 (LG71_01225) hpf 257994..258281 (+) 288 WP_043080894.1 ribosome hibernation promoting factor -
  LG71_RS01215 (LG71_01230) ptsN 258339..258830 (+) 492 WP_043080895.1 PTS IIA-like nitrogen regulatory protein PtsN -
  LG71_RS01220 (LG71_01235) rapZ 258864..259718 (+) 855 WP_043080896.1 RNase adapter RapZ -
  LG71_RS01225 (LG71_01240) npr 259715..259987 (+) 273 WP_043080897.1 PTS phosphocarrier protein NPr -
  LG71_RS01230 (LG71_01245) yrbL 260170..260802 (+) 633 WP_043080898.1 PhoP regulatory network protein YrbL -
  LG71_RS01235 (LG71_01250) mtgA 260804..261529 (-) 726 WP_043080899.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  LG71_RS01240 (LG71_01255) elbB 261526..262179 (-) 654 WP_043080900.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53606.41 Da        Isoelectric Point: 4.3084

>NTDB_id=129319 LG71_RS01205 WP_043086293.1 256538..257971(+) (rpoN/rpoN1) [Pluralibacter gergoviae strain FB2]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQTDLHEEVESPESADTEALDSVDALEQKEMPE
ELPLDASWDEIYTAGTPSGNGVDYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTIPIED
ILESIGDADIDIEEVEAVLKRIQRFDPIGVAAKDLRDCLLIQLSQYASDTPWLAEARSIVSDHLDLLANHDFRSLMRVTR
MREEVLKEAVTLIQSLDPRPGQSIQTGEPEYVIPDVLVRKVSGRWTVELNSDSIPRLQINQHYAGMCNSARSDADSQFIR
SNLQEAKWLIKSLESRNDTLLRVSRCIVEQQQAFFDQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVSTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=129319 LG71_RS01205 WP_043086293.1 256538..257971(+) (rpoN/rpoN1) [Pluralibacter gergoviae strain FB2]
ATGAAGCAAGGTTTGCAACTAAGGCTCAGCCAACAGCTCGCCATGACGCCGCAATTGCAGCAGGCTATTCGTCTGCTGCA
GCTCTCTACGCTGGAACTTCAGCAGGAGCTCCAGCAGGCGCTCGAGAGCAATCCCCTGCTTGAGCAAACCGATCTTCACG
AAGAAGTTGAGTCGCCTGAAAGTGCCGATACCGAAGCGCTAGACAGCGTCGATGCGCTTGAGCAGAAAGAGATGCCGGAG
GAGCTGCCTCTTGATGCCAGCTGGGATGAGATATACACCGCTGGCACCCCCTCGGGAAATGGCGTCGATTATCAGGACGA
CGAGCTGCCCGTCTATCAGGGTGAAACCACCCAGTCGCTGCAGGACTATCTGATGTGGCAGGTGGAGCTGACGCCCTTCT
CGGACACCGACCGCGCCATTGCCACCTCCATCGTTGATGCCGTAGACGACACCGGCTACCTCACTATCCCTATCGAAGAT
ATCCTTGAAAGCATCGGCGATGCGGATATCGACATTGAAGAAGTTGAAGCGGTCCTCAAGCGCATCCAGCGCTTCGATCC
GATTGGCGTGGCGGCGAAAGATCTCCGCGACTGCCTGCTGATTCAGCTCTCCCAGTACGCCAGCGATACGCCCTGGCTGG
CGGAAGCCAGAAGTATCGTCAGCGATCATCTGGATCTGCTGGCGAACCACGACTTTCGCAGCCTGATGCGCGTGACCCGC
ATGCGCGAGGAGGTGCTGAAAGAGGCGGTCACGCTTATACAGTCCCTCGACCCGCGTCCGGGACAGTCGATTCAGACCGG
CGAACCGGAGTACGTCATCCCCGACGTGCTGGTGCGCAAGGTGAGCGGCCGCTGGACCGTCGAACTGAACTCCGACAGCA
TTCCGCGCCTGCAGATTAACCAGCACTACGCCGGGATGTGCAATAGCGCGCGCAGCGACGCCGACAGCCAGTTTATCCGC
AGCAATCTGCAGGAAGCGAAGTGGCTGATTAAGAGCCTTGAGAGCCGCAACGATACCCTGCTGCGGGTCAGCCGCTGCAT
CGTTGAACAGCAGCAGGCGTTCTTTGATCAGGGCGAAGAGTTTATGAAGCCGATGGTGCTGGCGGACATCGCCCAGGCCG
TCGAGATGCACGAATCCACGATTTCTCGCGTCACCACGCAGAAATATCTCCACAGCCCGAGGGGCATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTAAGCACCGAGGGCGGCGGCGAAGCCTCCTCGACGGCCATTCGCGCGCTGGTGAAGAAATT
AATTGCCGCGGAAAATCCCGCGAAACCGCTGAGCGACAGTAAGCTGACTACCATGCTGTCCGAGCAGGGTATCATGGTGG
CACGCCGCACCGTTGCAAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAATCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A089PID0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48

Multiple sequence alignment