Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LC14_RS03585 Genome accession   NZ_CP009421
Coordinates   687170..688300 (+) Length   376 a.a.
NCBI ID   WP_002221204.1    Uniprot ID   A0AA93A8P8
Organism   Neisseria meningitidis strain NM3683     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 682170..693300
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LC14_RS13690 - 682835..682984 (+) 150 WP_019273745.1 hypothetical protein -
  LC14_RS03570 (LC14_03760) lepB 683203..684222 (-) 1020 WP_002221207.1 signal peptidase I -
  LC14_RS03575 (LC14_03765) lepA 684365..686158 (-) 1794 WP_002214015.1 translation elongation factor 4 -
  LC14_RS03580 (LC14_03770) - 686301..687002 (-) 702 WP_041423110.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  LC14_RS03585 (LC14_03775) pilU 687170..688300 (+) 1131 WP_002221204.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LC14_RS03590 (LC14_03780) - 688335..689312 (+) 978 WP_002221203.1 DNA polymerase III subunit delta' -
  LC14_RS03595 (LC14_03785) - 689316..689666 (+) 351 WP_002221202.1 PilZ domain-containing protein -
  LC14_RS03600 (LC14_03790) - 689671..690450 (+) 780 WP_002221201.1 TatD family hydrolase -
  LC14_RS03605 (LC14_03795) - 690476..690691 (+) 216 WP_002219531.1 DUF333 domain-containing protein -
  LC14_RS03610 (LC14_03800) grxD 690792..691103 (+) 312 WP_002214004.1 Grx4 family monothiol glutaredoxin -
  LC14_RS03620 (LC14_03810) upp 691209..691835 (+) 627 WP_002214002.1 uracil phosphoribosyltransferase -
  LC14_RS03625 (LC14_03815) - 691858..692178 (+) 321 WP_002214001.1 DUF2322 family protein -
  LC14_RS03630 (LC14_03820) - 692581..693003 (+) 423 WP_002213999.1 protein Gly1ORF1 -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42171.26 Da        Isoelectric Point: 6.2881

>NTDB_id=129127 LC14_RS03585 WP_002221204.1 687170..688300(+) (pilU) [Neisseria meningitidis strain NM3683]
MFTDENMTAKEELFAWLRHMNQNKGSDLFVTTHFPPAMKLDGKITRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECN
FAISLPDTSRFRVNAMIQRGATALVFRAITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHA
NSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKST
TLGMQTFDQHLYQLYEKGDISLQDALKNADSAHDLRLAVQLRSRRAQSSGPDLELL

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=129127 LC14_RS03585 WP_002221204.1 687170..688300(+) (pilU) [Neisseria meningitidis strain NM3683]
ATGTTTACCGATGAAAATATGACCGCAAAGGAAGAACTGTTCGCATGGCTGCGCCATATGAACCAAAACAAAGGTTCCGA
CCTGTTCGTGACGACTCATTTCCCGCCCGCAATGAAGCTGGACGGCAAAATCACCCGCATCACGGACGAACCGCTGACGG
CGGAAAAATGTATGGAAATCGCCTTTTCGATTATGAGTGCGAAGCAGGCGGAAGAATTTTCATCGACCAACGAGTGCAAC
TTCGCCATCAGTCTGCCGGACACCAGCCGCTTCCGCGTCAATGCGATGATACAGCGCGGCGCGACAGCGTTGGTATTCCG
TGCGATTACCAGCAAGATTCCCAAGTTTGAAAGCCTGAACCTGCCGCCGGTCTTGAAGGATGTCGCGCTGAAAAAACGCG
GGCTGGTTATTTTTGTCGGCGGCACCGGCTCGGGCAAATCGACTTCGCTTGCCTCGCTTATCGACTACCGCAATGAAAAT
TCGTTCGGACACATCATCACCATCGAAGACCCGATCGAGTTTGTCCACGAACACAAAAACTGCATCATCACCCAGCGCGA
GGTCGGCGTGGATACGGAAAACTGGATGGCGGCGTTGAAAAACACGCTGCGTCAGGCGCCGGATGTGATTCTGATCGGCG
AAATCCGCGACCGCGAAACGATGGACTACGCCATTGCCTTTGCCGAAACGGGGCATTTGTGTATGGCGACGCTGCACGCC
AACAGCACCAATCAGGCACTCGACCGCATCATCAACTTTTTCCCCGAGGAGCGGCGCGAACAATTGCTGACGGATTTGTC
GCTCAACCTTCAGGCGTTTATTTCGCAACGCCTCGTTCCGCGAGACGGCGGCAAGGGCAGGGTGGCGGCAGTCGAGGTGC
TGCTCAATTCGCCCCTGATTTCGGAGTTGATTCACAACGGCAACATCCATGAAATCAAAGAAGTGATGAAAAAATCCACT
ACCCTGGGTATGCAGACCTTCGACCAACACCTTTACCAATTGTATGAAAAAGGCGATATTTCCTTGCAGGATGCCTTGAA
AAATGCCGATTCGGCGCATGATTTGCGTTTGGCTGTTCAGTTGAGAAGCCGCCGCGCGCAAAGTTCCGGACCGGATTTGG
AATTGCTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(89-279)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.659

93.617

0.559
  pilU Acinetobacter baylyi ADP1

57.434

91.223

0.524
  pilU Vibrio cholerae strain A1552

54.318

95.479

0.519
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.343

93.883

0.407
  pilT Acinetobacter baylyi ADP1

43.03

87.766

0.378
  pilT Legionella pneumophila strain Lp02

43.519

86.17

0.375
  pilT Legionella pneumophila strain ERS1305867

43.519

86.17

0.375
  pilT Pseudomonas aeruginosa PAK

42.378

87.234

0.37
  pilT Acinetobacter baumannii strain A118

42.593

86.17

0.367
  pilT Acinetobacter nosocomialis M2

42.593

86.17

0.367
  pilT Acinetobacter baumannii D1279779

42.593

86.17

0.367
  pilT Neisseria meningitidis 8013

40.597

89.096

0.362
  pilT Pseudomonas stutzeri DSM 10701

41.463

87.234

0.362

Multiple sequence alignment