Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   AS86_RS33425 Genome accession   NZ_CP009351
Coordinates   5337732..5338166 (+) Length   144 a.a.
NCBI ID   WP_000811494.1    Uniprot ID   A0A9Q6M5X1
Organism   Bacillus thuringiensis HD1002     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 5332732..5343166
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AS86_RS33405 (AS86_5541) - 5333645..5334808 (-) 1164 WP_000434585.1 aminotransferase A -
  AS86_RS33410 (AS86_5542) - 5334930..5335130 (-) 201 WP_000929277.1 hypothetical protein -
  AS86_RS33415 (AS86_5543) kinB 5335441..5336715 (+) 1275 WP_000420367.1 sporulation sensor histidine kinase KinB -
  AS86_RS33420 (AS86_5544) - 5336754..5337503 (-) 750 WP_000388468.1 DUF3967 domain-containing protein -
  AS86_RS33425 (AS86_5545) nucA/comI 5337732..5338166 (+) 435 WP_000811494.1 DNA-entry nuclease Machinery gene
  AS86_RS33430 (AS86_5546) - 5338203..5338592 (+) 390 WP_000424022.1 competence protein ComJ -
  AS86_RS33435 (AS86_5547) metE 5338630..5340918 (-) 2289 WP_001007602.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  AS86_RS33445 (AS86_5548) - 5341431..5341988 (-) 558 WP_000062064.1 PadR family transcriptional regulator -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16054.43 Da        Isoelectric Point: 8.4331

>NTDB_id=128826 AS86_RS33425 WP_000811494.1 5337732..5338166(+) (nucA/comI) [Bacillus thuringiensis HD1002]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=128826 AS86_RS33425 WP_000811494.1 5337732..5338166(+) (nucA/comI) [Bacillus thuringiensis HD1002]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCATATCCTTCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGCGGAGCAGGCTCTTGGGTAGGGAATAAGTTAGATAAATATC
CAGATGGTACGCGTGTGAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549

Multiple sequence alignment