Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   AS86_RS31645 Genome accession   NZ_CP009351
Coordinates   5027192..5027938 (+) Length   248 a.a.
NCBI ID   WP_000487007.1    Uniprot ID   A0A9W3JRD4
Organism   Bacillus thuringiensis HD1002     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 5022192..5032938
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AS86_RS31615 (AS86_5187) - 5022481..5024625 (+) 2145 WP_000091515.1 HD family phosphohydrolase -
  AS86_RS31620 (AS86_5188) ybeY 5024622..5025092 (+) 471 WP_000054680.1 rRNA maturation RNase YbeY -
  AS86_RS31625 (AS86_5189) - 5025089..5025442 (+) 354 WP_000715475.1 diacylglycerol kinase family protein -
  AS86_RS31630 (AS86_5190) - 5025542..5025940 (+) 399 WP_001086287.1 cytidine deaminase -
  AS86_RS31635 (AS86_5191) era 5025933..5026838 (+) 906 WP_001080236.1 GTPase Era -
  AS86_RS31640 (AS86_5192) - 5027015..5027158 (+) 144 WP_000883924.1 YqzL family protein -
  AS86_RS31645 (AS86_5193) recO 5027192..5027938 (+) 747 WP_000487007.1 DNA repair protein RecO Machinery gene
  AS86_RS31650 (AS86_5194) - 5028046..5028678 (+) 633 WP_000583756.1 helix-turn-helix transcriptional regulator -
  AS86_RS31655 (AS86_5195) - 5028708..5029520 (+) 813 WP_000368949.1 pyruvate, water dikinase regulatory protein -
  AS86_RS37475 - 5030156..5030299 (+) 144 WP_000479749.1 hypothetical protein -
  AS86_RS31665 (AS86_5196) dnaG 5030381..5032177 (+) 1797 WP_000528221.1 DNA primase -

Sequence

Protein


Download         Length: 248 a.a.        Molecular weight: 28622.20 Da        Isoelectric Point: 8.0566

>NTDB_id=128822 AS86_RS31645 WP_000487007.1 5027192..5027938(+) (recO) [Bacillus thuringiensis HD1002]
MFQKVEGIVIRTTDYGETNKIVTIFSRELGKISAMARGAKKPKSRLASVSQLMTHGHFLIQMGSGLGTLQQGEIISTMKE
IREDIFLTAYASFIVELTDKATEDKKHNPYLFEMLYQTLHYMCEGVDSEVLSLIYQTKMLPVLGMRPYFDTCAICHQETD
FVAFSVREGGFLCSRHAEQDPYRIPVGEAVHKLLRLFFHFDLHRLGNVSVKDSTKKQMRLVLNTYYDEYCGIYLKSRRFL
EQLDKFQI

Nucleotide


Download         Length: 747 bp        

>NTDB_id=128822 AS86_RS31645 WP_000487007.1 5027192..5027938(+) (recO) [Bacillus thuringiensis HD1002]
ATGTTTCAAAAAGTTGAGGGCATCGTTATCCGTACGACAGATTACGGAGAAACGAACAAGATTGTTACAATATTCTCAAG
AGAACTTGGTAAGATAAGTGCAATGGCAAGAGGGGCAAAAAAACCGAAAAGCCGGTTAGCATCTGTGTCGCAATTAATGA
CACATGGCCATTTTCTTATTCAAATGGGATCAGGGCTTGGAACATTGCAACAAGGCGAGATTATTTCAACTATGAAAGAA
ATTCGCGAGGATATATTTTTAACTGCTTATGCATCATTTATTGTTGAGTTAACTGATAAAGCAACAGAAGATAAAAAACA
TAATCCGTATTTATTTGAAATGTTATATCAAACGTTGCATTATATGTGTGAGGGTGTTGATTCAGAAGTATTATCATTAA
TTTATCAAACGAAAATGCTTCCGGTATTAGGGATGCGCCCGTACTTTGATACATGTGCGATTTGTCATCAAGAAACAGAC
TTTGTCGCCTTCTCCGTCAGGGAAGGCGGTTTTCTGTGCTCACGTCATGCAGAGCAAGACCCATATCGTATACCGGTAGG
AGAAGCTGTTCATAAATTATTACGTCTTTTCTTCCATTTCGATTTACATAGGCTCGGAAATGTATCGGTAAAGGATAGCA
CAAAAAAACAAATGCGCTTAGTATTGAATACATATTATGATGAATATTGCGGAATTTATTTGAAATCAAGACGTTTCTTA
GAACAACTTGATAAGTTTCAAATATAA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-238)

Recombination protein O N terminal (RecO_N)

(1-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

55.466

99.597

0.552

Multiple sequence alignment