Detailed information NTG0128812

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: AS86_RS27265
• Genome accession: NZ_CP009351
• Coordinates: 4223035..4224384 (+)
• Length: 449 a.a.
• NCBI ID: WP_002083580.1
• Uniprot ID: -
• Organism: Bacillus thuringiensis HD1002
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4218035..4229384

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AS86_RS27245 (AS86_4327)	-	4219172..4219807 (-)	636	WP_000926669.1	YigZ family protein	-
AS86_RS27250 (AS86_4328)	-	4220044..4220886 (+)	843	WP_000684726.1	DegV family protein	-
AS86_RS27255 (AS86_4329)	-	4221059..4221373 (+)	315	WP_000400857.1	winged helix-turn-helix transcriptional regulator	-
AS86_RS27260 (AS86_4330)	-	4221522..4222907 (+)	1386	WP_000499483.1	NlpC/P60 family protein	-
AS86_RS27265 (AS86_4331)	comFA	4223035..4224384 (+)	1350	WP_002083580.1	ATP-dependent helicase ComFA	Machinery gene
AS86_RS27270 (AS86_4332)	-	4224384..4225088 (+)	705	WP_003255239.1	ComF family protein	-
AS86_RS27275 (AS86_4333)	cspC	4225215..4225412 (+)	198	WP_001990088.1	cold shock protein CspC	-
AS86_RS27280 (AS86_4334)	hpf	4225734..4226276 (+)	543	WP_000671187.1	ribosome hibernation-promoting factor, HPF/YfiA family	-
AS86_RS27285 (AS86_4335)	secA	4226549..4229056 (+)	2508	WP_000579374.1	preprotein translocase subunit SecA	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 51166.05 Da
• Isoelectric Point: 10.0230

>NTDB_id=128812 AS86_RS27265 WP_002083580.1 4223035..4224384(+) (comFA) [Bacillus thuringiensis HD1002]
MLAGKQLLLDELSSDLQRELNDLKRKGEVICVQGVKKKNSKYMCQRCGNVDQRLFASFLCRRCSKVCTYCRKCITMGRVS
ECAVLVRGIAERKREKKLNLLQWNGKLSTGQNLAAQGVVEAIKQKESFFIWAVCGAGKTEMLFYGINEALQKGERVCIAT
PRTDVVLELAPRLQEVFPYIKVAALYGGSVDKEKDAVLVVATTHQLLRYYRAFHVMVVDEIDAFPYCADQMLQYAVKQAM
KERAARIYLTATPDETWKRKFRKGEQKGVIVSGRYHRHPLPVPLFCWCGNWKKSLIHKRIPRVLLQWLQTYLNKKHPIFL
FVPHVRYIEEISLLLKSLNKRIEGVHAEDPGRKEKVAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEAPYGEVIYFHYGKTEAMVRAKKHIQGMNKNAKEQGLID

Nucleotide

• Length: 1350 bp

>NTDB_id=128812 AS86_RS27265 WP_002083580.1 4223035..4224384(+) (comFA) [Bacillus thuringiensis HD1002]
ATGCTGGCGGGGAAACAGTTGCTATTAGACGAACTTTCTTCAGATTTACAGAGGGAATTAAATGATTTGAAAAGGAAGGG
AGAGGTCATATGTGTACAAGGTGTAAAAAAGAAGAATTCTAAATATATGTGCCAACGCTGCGGAAATGTAGATCAGCGGC
TATTTGCGTCGTTTTTATGTAGAAGGTGCAGTAAAGTGTGCACATATTGCCGGAAGTGTATAACGATGGGGAGGGTAAGT
GAATGTGCTGTACTTGTTCGCGGGATTGCTGAAAGAAAGAGAGAAAAGAAATTAAACTTGTTACAGTGGAACGGGAAGTT
GTCTACTGGTCAGAATTTGGCGGCACAAGGTGTTGTAGAGGCTATTAAGCAAAAAGAATCATTTTTTATTTGGGCTGTAT
GCGGGGCTGGGAAAACAGAGATGTTGTTTTACGGTATTAACGAAGCGCTTCAAAAAGGAGAAAGAGTTTGTATCGCAACG
CCGAGAACGGATGTTGTTCTGGAATTAGCACCGAGATTACAAGAAGTATTTCCATATATAAAGGTAGCGGCTTTATATGG
AGGGAGTGTGGATAAAGAAAAAGATGCAGTACTAGTCGTTGCGACCACGCATCAATTATTGCGTTATTATAGGGCGTTTC
ATGTCATGGTTGTAGATGAGATAGATGCTTTTCCATATTGTGCAGATCAAATGTTACAGTACGCGGTAAAACAAGCGATG
AAAGAAAGGGCGGCGCGTATTTATTTAACTGCGACTCCAGATGAAACGTGGAAGCGAAAATTTAGAAAAGGTGAACAAAA
AGGTGTTATTGTTTCTGGACGATATCACCGTCATCCTTTGCCAGTTCCTTTATTTTGTTGGTGCGGGAATTGGAAAAAAA
GCCTCATTCATAAAAGAATTCCTCGAGTTTTACTACAGTGGTTACAAACATACTTAAATAAAAAACATCCTATTTTTTTA
TTTGTCCCCCATGTGCGATATATAGAAGAGATAAGCCTGTTGTTAAAATCATTAAACAAGCGAATTGAAGGTGTACATGC
AGAAGATCCGGGGAGAAAAGAAAAAGTAGCGGCTTTCAGAAAAGGAGAAATCCCATTATTAGTTACAACGACAATTTTAG
AGCGAGGCGTAACGGTGAAAAATTTGCAAGTAGCGGTTTTAGGGGCTGAAGAAGAAATATTCTCAGAAAGTGCACTCGTA
CAAATTGCGGGCCGAGCAGGGCGGAGCTTTGAAGCACCGTATGGAGAGGTCATTTATTTTCACTATGGTAAGACAGAGGC
GATGGTGCGCGCGAAAAAACATATTCAAGGTATGAATAAAAATGCCAAAGAACAAGGATTGATCGATTAA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (107-254)

• Helicase conserved C-terminal domain (Helicase_C) (311-408)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	51.131	98.441	0.503
comFA	Latilactobacillus sakei subsp. sakei 23K	41.709	88.641	0.37