Detailed information NTG0128672

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: BF38_RS07685
• Genome accession: NZ_CP009335
• Coordinates: 862942..864291 (-)
• Length: 449 a.a.
• NCBI ID: WP_000879417.1
• Uniprot ID: A0AAE9TF02
• Organism: Bacillus thuringiensis strain HD1011
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 857942..869291

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BF38_RS07665 (BF38_916)	secA	858263..860770 (-)	2508	WP_000579378.1	preprotein translocase subunit SecA	-
BF38_RS07670 (BF38_917)	hpf	861047..861589 (-)	543	WP_000671195.1	ribosome hibernation-promoting factor, HPF/YfiA family	-
BF38_RS07675 (BF38_918)	cspC	861914..862111 (-)	198	WP_001990088.1	cold shock protein CspC	-
BF38_RS07680 (BF38_919)	comFC	862238..862942 (-)	705	WP_003162242.1	comF operon protein ComFC	-
BF38_RS07685 (BF38_920)	comFA	862942..864291 (-)	1350	WP_000879417.1	ATP-dependent helicase ComFA	Machinery gene
BF38_RS07690 (BF38_921)	-	864419..865729 (-)	1311	WP_000734329.1	NlpC/P60 family protein	-
BF38_RS07695 (BF38_922)	-	865982..866296 (-)	315	WP_000400857.1	winged helix-turn-helix transcriptional regulator	-
BF38_RS07705 (BF38_923)	-	866470..867312 (-)	843	WP_000844769.1	DegV family protein	-
BF38_RS07710 (BF38_924)	-	867552..868187 (+)	636	WP_000926685.1	YigZ family protein	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 51233.89 Da
• Isoelectric Point: 9.5047

>NTDB_id=128672 BF38_RS07685 WP_000879417.1 862942..864291(-) (comFA) [Bacillus thuringiensis strain HD1011]
MLAGKQLLLEELPSDLRRELSDLKKEGEVICVQGVIKKASKYICQRCGNIEQRLFASFLCKRCSKVCTYCRKCITMGRVS
ECAVLVRGIHERKGERELHSLQWKGSLSLGQELAAQGVIEAIKQKESFFIWAVCGAGKTEMLFYGIEEALQKGERVCIAT
PRTDVVLELVPRLQEVFPSINVAALYGGSVDREKDAALVVATTHQLLRYYRAFHVMIVDEIDAFPYHADQMLQYAVQQAM
KEKAARIYLTATPDEKWKRNFRTGKQKGIIVSGRYHRHPLPVPLFSWCGNWKKSLHHKKIPRVLLQWLKMYVNKKYPIFL
FVPHVRYIEEIGLLLKGLDHRIDGVHAEDPMRKEKVEAFRKGDIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEEPYGEVVYFHYGKTESMVRAKRHIQSMNKSAKEQGLID

Nucleotide

• Length: 1350 bp

>NTDB_id=128672 BF38_RS07685 WP_000879417.1 862942..864291(-) (comFA) [Bacillus thuringiensis strain HD1011]
ATGCTCGCTGGAAAGCAGTTACTATTAGAAGAACTCCCTTCAGATTTACGGAGAGAATTAAGTGATTTGAAAAAGGAGGG
AGAGGTCATATGTGTACAAGGCGTAATAAAGAAGGCTTCTAAATATATATGTCAGCGCTGCGGAAATATAGAGCAGCGGC
TATTTGCATCATTTTTATGTAAAAGGTGCAGTAAAGTATGTACGTATTGCCGGAAGTGTATAACGATGGGGAGAGTTAGT
GAATGTGCTGTACTTGTTCGCGGGATTCATGAAAGAAAAGGAGAAAGGGAATTACATTCGTTACAGTGGAAAGGGAGTTT
GTCTCTTGGTCAGGAGCTGGCGGCGCAAGGTGTTATAGAAGCTATTAAGCAAAAAGAATCTTTTTTTATTTGGGCTGTGT
GCGGGGCTGGAAAAACAGAAATGTTATTTTACGGTATAGAAGAGGCACTTCAAAAAGGAGAAAGAGTGTGTATCGCAACG
CCAAGGACGGACGTTGTACTGGAATTAGTACCGAGATTACAAGAAGTGTTTCCAAGTATAAATGTAGCTGCTTTATACGG
AGGGAGTGTAGATCGTGAAAAAGATGCAGCGTTAGTCGTTGCAACGACGCATCAATTGTTACGTTATTATAGAGCGTTTC
ATGTAATGATTGTAGATGAGATTGATGCCTTCCCGTATCATGCGGATCAAATGTTACAGTATGCAGTGCAGCAAGCGATG
AAAGAGAAAGCAGCGCGTATTTATTTAACTGCAACCCCTGATGAAAAGTGGAAGCGCAATTTCAGAACGGGGAAACAAAA
AGGTATCATTGTCTCTGGACGATACCATCGTCATCCGTTACCAGTTCCTCTATTTAGTTGGTGCGGAAATTGGAAGAAAA
GCCTCCATCATAAAAAAATTCCTCGTGTGTTACTACAATGGTTAAAAATGTACGTAAACAAAAAATACCCTATTTTTTTA
TTTGTTCCTCATGTGCGATATATAGAAGAAATAGGCCTGTTATTGAAAGGGTTGGATCATAGAATCGATGGCGTACATGC
AGAAGATCCGATGAGAAAAGAAAAAGTGGAAGCGTTTCGAAAGGGAGACATTCCGTTATTAGTTACAACGACAATTTTAG
AAAGAGGGGTAACTGTGAAGAACTTACAAGTGGCTGTGTTAGGGGCTGAAGAAGAAATATTTTCAGAAAGTGCGCTCGTA
CAAATTGCAGGCCGAGCAGGTCGTAGTTTTGAAGAACCGTATGGTGAGGTTGTTTATTTTCATTACGGTAAGACAGAGTC
GATGGTACGTGCGAAAAGACATATTCAAAGTATGAACAAAAGTGCGAAAGAACAAGGATTAATTGATTAA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (108-254)

• Helicase conserved C-terminal domain (Helicase_C) (312-408)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	51.914	93.096	0.483
comFA	Latilactobacillus sakei subsp. sakei 23K	40.704	88.641	0.361