Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   BF38_RS03295 Genome accession   NZ_CP009335
Coordinates   52498..53244 (-) Length   248 a.a.
NCBI ID   WP_000487010.1    Uniprot ID   Q81LT9
Organism   Bacillus thuringiensis strain HD1011     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 47498..58244
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BF38_RS03270 (BF38_50) dnaG 48266..50062 (-) 1797 WP_000528212.1 DNA primase -
  BF38_RS34045 - 50393..50548 (-) 156 WP_001179661.1 hypothetical protein -
  BF38_RS03285 (BF38_51) - 50916..51728 (-) 813 WP_000368943.1 pyruvate, water dikinase regulatory protein -
  BF38_RS03290 (BF38_52) - 51758..52390 (-) 633 WP_000583759.1 helix-turn-helix transcriptional regulator -
  BF38_RS03295 (BF38_53) recO 52498..53244 (-) 747 WP_000487010.1 DNA repair protein RecO Machinery gene
  BF38_RS03300 (BF38_54) - 53278..53421 (-) 144 WP_000883924.1 YqzL family protein -
  BF38_RS03305 (BF38_55) era 53598..54503 (-) 906 WP_001080241.1 GTPase Era -
  BF38_RS03310 (BF38_56) - 54496..54894 (-) 399 WP_001086294.1 cytidine deaminase -
  BF38_RS03315 (BF38_57) - 54994..55347 (-) 354 WP_000715480.1 diacylglycerol kinase family protein -
  BF38_RS03320 (BF38_58) ybeY 55344..55814 (-) 471 WP_000054692.1 rRNA maturation RNase YbeY -
  BF38_RS03325 (BF38_59) - 55811..57955 (-) 2145 WP_000946454.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 248 a.a.        Molecular weight: 28665.22 Da        Isoelectric Point: 8.0540

>NTDB_id=128662 BF38_RS03295 WP_000487010.1 52498..53244(-) (recO) [Bacillus thuringiensis strain HD1011]
MFQKVEGIVIRTTDYGETNKIVTIFSRELGKVSAMARGAKKPKSRLASVSQLMTHGHFLIQMGSGLGTLQQGEIISTMKE
IREDIFLTAYASFIVELTDKATEDKKHNPYLFEMLYQTLHYMCEGVDPEVLSLIYQTKMLPVLGMRPYFDTCAICHQETD
FVAFSVREGGFLCSRHAEQDQYRIPVGEAVHKLLRLFYHFDLHRLGNVSVKDSTKKQMRLVLNTYYDEYCGIYLKSRRFL
EQLDKFQI

Nucleotide


Download         Length: 747 bp        

>NTDB_id=128662 BF38_RS03295 WP_000487010.1 52498..53244(-) (recO) [Bacillus thuringiensis strain HD1011]
ATGTTTCAAAAAGTTGAGGGCATCGTTATCCGTACGACAGATTACGGAGAAACGAACAAGATTGTTACAATATTTTCAAG
AGAATTGGGTAAGGTAAGTGCAATGGCAAGAGGAGCGAAAAAACCGAAAAGTCGGTTAGCATCTGTTTCGCAACTTATGA
CACATGGTCATTTTCTTATTCAAATGGGATCTGGACTTGGAACTTTGCAACAAGGCGAGATTATTTCAACTATGAAAGAA
ATTCGCGAGGATATATTTTTAACTGCTTATGCATCATTTATTGTTGAATTAACTGATAAAGCAACAGAAGATAAAAAACA
TAATCCATATTTATTTGAAATGTTATATCAAACGTTGCATTATATGTGTGAGGGTGTTGATCCAGAAGTATTATCATTAA
TTTATCAAACGAAAATGCTTCCGGTATTAGGGATGCGCCCGTACTTTGACACATGTGCGATTTGTCATCAAGAAACAGAT
TTTGTCGCCTTCTCTGTTAGAGAGGGCGGTTTTCTGTGCTCACGTCATGCTGAGCAAGATCAGTATCGTATACCAGTGGG
GGAAGCCGTTCATAAATTATTGCGTCTTTTCTACCACTTCGATTTACATAGACTTGGCAATGTATCAGTGAAGGATAGCA
CAAAAAAACAAATGCGTTTAGTATTGAATACATATTATGATGAATATTGCGGGATTTATTTGAAATCAAGACGTTTCTTA
GAACAACTTGATAAGTTTCAAATATAA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-238)

Recombination protein O N terminal (RecO_N)

(1-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q81LT9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

55.466

99.597

0.552

Multiple sequence alignment