Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   BF38_RS03020 Genome accession   NZ_CP009335
Coordinates   208..1251 (-) Length   347 a.a.
NCBI ID   WP_001013197.1    Uniprot ID   A0AAE9TCD1
Organism   Bacillus thuringiensis strain HD1011     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1..6251
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BF38_RS03020 (BF38_1) comGA 208..1251 (-) 1044 WP_001013197.1 competence protein ComGA Machinery gene
  BF38_RS03025 (BF38_2) - 1457..2152 (+) 696 WP_000434117.1 helix-turn-helix transcriptional regulator -
  BF38_RS03030 (BF38_3) - 2278..2520 (+) 243 WP_000440711.1 DUF2626 domain-containing protein -
  BF38_RS03035 (BF38_4) - 2628..4022 (+) 1395 WP_001094329.1 L-cystine transporter -
  BF38_RS03040 (BF38_5) - 4254..4469 (-) 216 WP_001008320.1 DUF3912 family protein -
  BF38_RS03045 (BF38_6) - 4722..5033 (+) 312 WP_001093243.1 hypothetical protein -
  BF38_RS03050 (BF38_7) - 5070..5558 (-) 489 WP_000764252.1 hypothetical protein -
  BF38_RS03055 (BF38_8) - 5638..6012 (-) 375 WP_001012351.1 nucleoside 2-deoxyribosyltransferase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39397.95 Da        Isoelectric Point: 9.0635

>NTDB_id=128661 BF38_RS03020 WP_001013197.1 208..1251(-) (comGA) [Bacillus thuringiensis strain HD1011]
MNGIESFANMILKEACRVQASDLHIVPRQKDVVVQLRIGKDLMTKYCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQMDGQEVYLRLSTLPTVYQESLVIRLHLQASIQPLSHLSLFPSTAKILLSFLRYSHGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIVTLEDPVEKRNDDVLQIQINEKAGITYEAGLKAILRHDPDVILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDARGAILRFMDFGITRQEIEQSLLAIAAQRLVELKCPFCKRKCSTLCKSMRQVRQASIYELLYGYELKQAIKEANGEC
VTYKHETLQSSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=128661 BF38_RS03020 WP_001013197.1 208..1251(-) (comGA) [Bacillus thuringiensis strain HD1011]
ATGAATGGAATTGAAAGCTTTGCGAATATGATTTTGAAAGAAGCGTGCAGGGTACAAGCGTCGGACTTACATATTGTGCC
CCGGCAGAAGGATGTAGTGGTTCAGCTGCGTATAGGAAAAGATTTAATGACGAAATACTGTATTGAAAAGGAATTTGGAG
AAAAACTTGTTTCACACTTTAAATTTTTAGCATCTATGGATATAGGGGAGAGGCGGAAGCCACAAAATGGTTCACTGTAT
TTACAAATGGATGGACAAGAAGTGTATTTGCGCCTTTCCACGCTTCCAACCGTATACCAAGAAAGTCTCGTTATTCGTCT
TCATTTACAAGCATCTATTCAGCCGTTATCTCATCTTTCGTTATTTCCAAGTACAGCGAAGATACTACTTTCTTTTTTAC
GTTATTCGCATGGATTACTCGTATTTACTGGACCGACTGGTTCGGGGAAGACAACAACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATTGTTACACTGGAAGATCCAGTTGAAAAAAGAAATGACGATGTATTACAAATTCA
AATAAATGAAAAAGCAGGTATCACATATGAGGCTGGACTAAAGGCTATTTTGCGTCATGATCCAGATGTTATTTTAGTCG
GTGAAATTCGTGATGAAGAAACAGCGAAAATTGCTATAAGAGCAAGTTTGACTGGCCATTTAGTAATGACGACATTGCAT
ACGAATGATGCGAGAGGGGCGATACTTAGGTTCATGGATTTTGGCATAACGAGGCAAGAAATCGAACAATCTTTATTAGC
TATAGCTGCACAGCGACTTGTCGAATTAAAGTGTCCGTTTTGCAAAAGAAAGTGCTCAACTTTATGCAAATCAATGAGGC
AAGTAAGGCAAGCGAGTATTTATGAGTTGTTATATGGATATGAGTTAAAACAAGCGATTAAAGAAGCAAACGGGGAATGT
GTCACATACAAGCACGAAACATTACAATCTTCGATACGAAAAGGATACGCTTTAGGGTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.925

100

0.579

Multiple sequence alignment