Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BM43_RS21695 Genome accession   NZ_CP009323
Coordinates   1083253..1084758 (+) Length   501 a.a.
NCBI ID   WP_036049140.1    Uniprot ID   A0AAW7R6G5
Organism   Burkholderia gladioli strain ATCC 10248     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1078253..1089758
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BM43_RS21670 (BM43_987) kdsD 1079462..1080445 (+) 984 WP_036049146.1 arabinose 5-phosphate isomerase KdsD -
  BM43_RS21675 (BM43_988) - 1080445..1080984 (+) 540 WP_036049144.1 KdsC family phosphatase -
  BM43_RS21680 (BM43_989) lptC 1080988..1081602 (+) 615 WP_017918294.1 LPS export ABC transporter periplasmic protein LptC -
  BM43_RS21685 (BM43_990) lptA 1081621..1082289 (+) 669 WP_036049142.1 lipopolysaccharide transport periplasmic protein LptA -
  BM43_RS21690 (BM43_991) lptB 1082286..1083062 (+) 777 WP_013699406.1 LPS export ABC transporter ATP-binding protein -
  BM43_RS21695 (BM43_992) rpoN/rpoN1 1083253..1084758 (+) 1506 WP_036049140.1 RNA polymerase factor sigma-54 Machinery gene
  BM43_RS21700 (BM43_993) hpf 1084909..1085274 (+) 366 WP_013699408.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  BM43_RS21705 (BM43_994) - 1085488..1086000 (+) 513 WP_025097001.1 PTS sugar transporter subunit IIA -
  BM43_RS21710 (BM43_995) hprK 1086126..1087094 (+) 969 WP_013699410.1 HPr(Ser) kinase/phosphatase -
  BM43_RS21715 (BM43_996) rapZ 1087205..1088098 (+) 894 WP_013699411.1 RNase adapter RapZ -
  BM43_RS21720 (BM43_997) - 1088131..1088766 (+) 636 WP_036049137.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 55077.04 Da        Isoelectric Point: 4.9483

>NTDB_id=128511 BM43_RS21695 WP_036049140.1 1083253..1084758(+) (rpoN/rpoN1) [Burkholderia gladioli strain ATCC 10248]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDEWIASPLRVAADGSLIAQSPPSSTPEPMHG
GGSNGSEASGDRAERDDSRNGDDYDYNADSGDSGQWNLDDYGRSSGASDDDDLPPLQVHEASTSLREHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLGAGLDEVLTDLPEELEVDLDELGAALALLHSFDPAGVGARSASECLRLQLLRLDPSPTRTL
SLDIVSQHLELLAARDFTRLRKQLKANDDELREAHALIRSLEPFPGAAYGKTEADYVVPDIMVRKSGQNWLAELNPEVVP
KLRINHLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDQKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=128511 BM43_RS21695 WP_036049140.1 1083253..1084758(+) (rpoN/rpoN1) [Burkholderia gladioli strain ATCC 10248]
ATGAAAGCCAGCCTCCAACTTCGCCTGTCGCAGCACCTCGCGCTCACGCCCCAGCTCCAGCAGTCGATCCGGCTGCTGCA
GCTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGATGGCCGTCGCCCAGAATCCGCTGCTCGAGAACGACGAATGGATCG
CGAGCCCGCTGCGGGTGGCCGCCGACGGCTCGCTGATCGCGCAGAGCCCGCCCTCCTCGACGCCCGAGCCGATGCACGGT
GGCGGCTCGAACGGCAGCGAAGCCTCGGGCGATCGCGCCGAACGCGACGACTCGCGCAATGGCGACGACTACGACTACAA
CGCCGACAGCGGCGATTCGGGTCAGTGGAATCTCGACGACTACGGTCGCTCCTCGGGCGCCTCCGACGACGACGACCTCC
CGCCGCTGCAGGTGCACGAGGCCTCGACCTCGCTGCGCGAGCATCTGTCCGCGCAATTGCGGGTGACCCAGGCGGGTCCG
CGCGACCGCGCGCTGGTGATGTTCCTGATCGAATCGCTCGACGACGACGGTTATCTCGGCGCGGGGCTCGACGAGGTGTT
GACCGACCTGCCCGAGGAACTGGAAGTCGATCTCGACGAACTGGGCGCCGCGCTGGCGCTGCTGCACAGCTTCGACCCGG
CCGGCGTGGGGGCGCGCTCCGCATCGGAATGCTTGCGCCTGCAATTGTTGCGACTCGATCCGTCCCCCACGCGCACGCTG
TCGCTCGACATCGTCTCGCAGCATCTCGAATTGCTGGCCGCGCGCGACTTCACGCGGCTGCGCAAGCAGTTGAAGGCCAA
CGACGACGAGCTGCGCGAGGCCCATGCACTGATCCGCTCGCTCGAGCCCTTCCCGGGCGCCGCCTACGGCAAGACCGAGG
CGGACTACGTGGTGCCCGACATCATGGTCAGGAAGAGCGGCCAGAACTGGCTCGCGGAGCTCAATCCCGAGGTGGTGCCG
AAGCTGCGCATCAACCACCTGTACGCCAATATCCTGCGCAACAGCCGGGGCGATCCGAGCGCCGGTTCGCTCAAGCAGCA
GCTCCAGGAAGCGCGCTGGCTGATCAAGAATATCCAGCAGCGATTCGAGACCATCCTGCGTGTCGCGCAGGCCATCGTGG
AGCGTCAGAAGAACTTTTTTGCGCATGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCTCCCGTGTGACAACCGGCAAGTACATGCTGACCCCGTTCGGCACTCTTGAATTTAAGTACTT
CTTCGGATCACACGTGTCGACGGATACCGGGGGCGCTGCCTCTTCCACGGCAATCCGCGCCCTCATCAAGCAACTGATAG
GAGCCGAAGACCAGAAATCTCCTCTTTCGGACAGCCGAATAGCCGAGCTGCTGGCGGAACAAGGCTTCGTGGTCGCGCGC
CGCACCGTTGCGAAGTATCGCGAAGCCCTCAAGATCCCCGCGGTGAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.182

100

0.689

Multiple sequence alignment