Detailed information NTG0128505

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: AS54_RS27185
• Genome accession: NZ_CP009318
• Coordinates: 5009409..5010758 (-)
• Length: 449 a.a.
• NCBI ID: WP_000225366.1
• Uniprot ID: A0A158RMK4
• Organism: Bacillus cereus 03BB102
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5004409..5015758

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AS54_RS27165 (AS54_5240)	secA	5004731..5007238 (-)	2508	WP_000579383.1	preprotein translocase subunit SecA	-
AS54_RS27170 (AS54_5241)	hpf	5007515..5008057 (-)	543	WP_000671189.1	ribosome hibernation-promoting factor, HPF/YfiA family	-
AS54_RS27175 (AS54_5242)	cspC	5008381..5008578 (-)	198	WP_001990088.1	cold shock protein CspC	-
AS54_RS27180 (AS54_5243)	comFC	5008705..5009409 (-)	705	WP_003162242.1	comF operon protein ComFC	-
AS54_RS27185 (AS54_5244)	comFA	5009409..5010758 (-)	1350	WP_000225366.1	ATP-dependent helicase ComFA	Machinery gene
AS54_RS27190 (AS54_5245)	-	5010886..5012196 (-)	1311	WP_000734330.1	NlpC/P60 family protein	-
AS54_RS27195 (AS54_5246)	-	5012450..5012764 (-)	315	WP_000400857.1	winged helix-turn-helix transcriptional regulator	-
AS54_RS27205 (AS54_5247)	-	5012938..5013780 (-)	843	WP_000844766.1	DegV family protein	-
AS54_RS27210 (AS54_5248)	-	5014020..5014655 (+)	636	WP_000926685.1	YigZ family protein	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 51428.10 Da
• Isoelectric Point: 9.6542

>NTDB_id=128505 AS54_RS27185 WP_000225366.1 5009409..5010758(-) (comFA) [Bacillus cereus 03BB102]
MVAGKQLLLEELSSDLRRELSDLKKKGEIICVQGVIKKASKYICQRCGNIEQRLFASFLCKRCSKVCTYCRKCITMGRVS
ECAVLVRGIHERKGERELHSLQWKGTLSTGQELAAQGVIEAIKQKESFFIWAVCGAGKTEMLFYGIEEALQKEERVCIAT
PRTDVVLELVPRLQEVFPSINVAALYGGSVDREKDAALVVATTHQLLRYYRAFHVMIVDEIDAFPYHADQMLQYAVQQAM
KERAARIYLTATPDEKWKRNFRRGKQKGIIVSGRYHRHPLPVPLFSWCGNWKKSLHHKKIPRVLLQWLKMYVNKKYPIFL
FVPHVRYIEEMSLLLKGLDHRIDGVHAEDPMRKEKVEAFRKGDIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEEPYGEVVYFHYGKTESMVRAKRHIQSMNKSAKEQGLID

Nucleotide

• Length: 1350 bp

>NTDB_id=128505 AS54_RS27185 WP_000225366.1 5009409..5010758(-) (comFA) [Bacillus cereus 03BB102]
ATGGTTGCTGGAAAGCAGTTACTATTAGAAGAACTCTCTTCAGATTTACGGAGAGAATTAAGTGATTTGAAAAAGAAGGG
AGAGATCATATGTGTACAAGGCGTAATAAAGAAGGCTTCTAAATATATATGTCAGCGCTGCGGAAATATAGAGCAGCGGC
TATTTGCATCATTTTTATGTAAAAGGTGCAGTAAAGTATGTACGTATTGCCGGAAGTGTATAACGATGGGGAGAGTTAGT
GAATGTGCTGTACTTGTTCGCGGAATTCATGAAAGAAAAGGAGAAAGAGAATTACATTCGTTACAGTGGAAAGGAACTTT
GTCTACTGGTCAGGAGTTGGCAGCGCAAGGTGTTATAGAAGCTATTAAGCAGAAAGAATCGTTTTTTATTTGGGCGGTAT
GCGGGGCTGGAAAAACAGAAATGTTATTTTACGGTATAGAAGAGGCACTTCAAAAAGAAGAAAGAGTTTGTATTGCAACG
CCAAGGACGGACGTTGTACTGGAATTAGTACCGAGATTACAAGAAGTGTTTCCAAGTATAAATGTAGCTGCTTTATACGG
AGGGAGTGTAGATCGTGAAAAAGATGCAGCGTTAGTCGTTGCAACGACTCACCAACTGTTACGTTACTATAGAGCATTCC
ATGTGATGATTGTAGATGAGATTGATGCCTTCCCGTATCATGCGGATCAAATGTTACAGTATGCAGTGCAACAAGCGATG
AAAGAGAGAGCGGCGCGTATTTATTTAACTGCAACCCCGGATGAAAAGTGGAAGCGCAATTTCAGAAGGGGGAAACAAAA
AGGTATCATTGTCTCTGGACGATACCATCGTCATCCGTTACCAGTTCCTCTATTTAGTTGGTGCGGTAATTGGAAGAAAA
GCCTTCATCATAAAAAAATTCCTCGAGTGTTACTACAATGGTTGAAAATGTACGTAAACAAAAAATATCCTATTTTTTTA
TTTGTTCCTCATGTGCGATATATAGAAGAAATGAGCCTGTTATTGAAAGGGTTGGATCATAGAATCGATGGCGTACATGC
AGAAGATCCGATGAGAAAAGAAAAAGTGGAAGCGTTTAGAAAGGGAGACATTCCGTTATTAGTTACAACGACAATTTTAG
AAAGGGGGGTAACTGTGAAGAACTTACAAGTGGCTGTGTTAGGGGCTGAAGAAGAAATATTTTCAGAAAGTGCGCTCGTA
CAAATTGCAGGCCGAGCAGGTCGTAGTTTTGAAGAACCGTATGGTGAGGTTGTTTATTTTCACTACGGCAAGACAGAGTC
GATGGTACGTGCGAAAAGACATATTCAAAGTATGAACAAAAGTGCGAAAGAACAAGGATTAATTGATTAA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (107-254)

• Helicase conserved C-terminal domain (Helicase_C) (312-408)

3D structure

• AlphaFold DB: A0A158RMK4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	51.914	93.096	0.483
comFA	Latilactobacillus sakei subsp. sakei 23K	40.5	89.087	0.361