Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   AS54_RS21230 Genome accession   NZ_CP009318
Coordinates   3935841..3936275 (-) Length   144 a.a.
NCBI ID   WP_000811507.1    Uniprot ID   -
Organism   Bacillus cereus 03BB102     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3930841..3941275
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AS54_RS21210 (AS54_4066) - 3931605..3932162 (+) 558 WP_000062080.1 PadR family transcriptional regulator -
  AS54_RS21220 (AS54_4067) metE 3933090..3935378 (+) 2289 WP_001007632.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  AS54_RS21225 (AS54_4068) comJ 3935415..3935804 (-) 390 WP_000424033.1 competence protein ComJ -
  AS54_RS21230 (AS54_4069) nucA/comI 3935841..3936275 (-) 435 WP_000811507.1 DNA-entry nuclease Machinery gene
  AS54_RS21235 (AS54_4070) - 3936512..3937261 (+) 750 WP_000388464.1 DUF3967 domain-containing protein -
  AS54_RS21240 (AS54_4071) kinB 3937315..3938589 (-) 1275 WP_000420389.1 sporulation sensor histidine kinase KinB -
  AS54_RS21245 (AS54_4072) - 3938900..3939100 (+) 201 WP_000929283.1 hypothetical protein -
  AS54_RS21250 (AS54_4073) - 3939223..3940386 (+) 1164 WP_000434590.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15998.32 Da        Isoelectric Point: 8.4331

>NTDB_id=128491 AS54_RS21230 WP_000811507.1 3935841..3936275(-) (nucA/comI) [Bacillus cereus 03BB102]
MKQLKGIIISIIAILSIVVAVYEVLVPEETSTKKTNAYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=128491 AS54_RS21230 WP_000811507.1 3935841..3936275(-) (nucA/comI) [Bacillus cereus 03BB102]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTGTAGTAGCGGTTTATGAAGTACTTGTTCC
AGAGGAAACAAGTACAAAAAAAACAAATGCGTATGATCAAGTTCTAGAATTTCCAAAAGAGAGATATCCAGAAACAGGGA
AACATATTACGGATGCGATAAAGGAAGGACATTCAGAAGTTTGTACAATCGACCGTGGTGGTGCTGCGGATAGAAGAAAG
TTATCGTTAGCTCCGTACCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGAGCACATATCGAATATATAAGTCCAGCAGATAACCGCGGAGCAGGGTCTTGGGTAGGAAATAAGTTAGATAAATACC
CAGATGGTACGCGTGTGAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

70.796

78.472

0.556

Multiple sequence alignment