Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   AS54_RS06950 Genome accession   NZ_CP009318
Coordinates   1180387..1184112 (+) Length   1241 a.a.
NCBI ID   WP_000572292.1    Uniprot ID   A0RAX7
Organism   Bacillus cereus 03BB102     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1175387..1189112
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AS54_RS06935 (AS54_1220) - 1175545..1176138 (+) 594 WP_000347516.1 TVP38/TMEM64 family protein -
  AS54_RS06940 (AS54_1221) lepB 1176195..1176758 (+) 564 WP_000751894.1 signal peptidase I -
  AS54_RS06945 (AS54_1222) addB 1176875..1180390 (+) 3516 WP_000058559.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  AS54_RS06950 (AS54_1223) addA 1180387..1184112 (+) 3726 WP_000572292.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  AS54_RS06955 (AS54_1224) - 1184125..1184412 (+) 288 WP_000718623.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  AS54_RS06960 (AS54_1225) gerPF 1184450..1184665 (-) 216 WP_001141566.1 spore germination protein GerPF -
  AS54_RS06965 (AS54_1226) - 1184708..1185094 (-) 387 WP_000902341.1 spore germination protein GerPE -
  AS54_RS06970 (AS54_1227) gerPD 1185110..1185304 (-) 195 WP_001052797.1 spore germination protein GerPD -
  AS54_RS06975 (AS54_1228) gerPC 1185311..1185925 (-) 615 WP_001070766.1 spore germination protein GerPC -
  AS54_RS06980 (AS54_1229) gerPB 1185993..1186199 (-) 207 WP_001012512.1 spore germination protein GerPB -
  AS54_RS06985 (AS54_1230) gerPA 1186214..1186435 (-) 222 WP_001111188.1 spore germination protein GerPA -
  AS54_RS06990 (AS54_1231) - 1186532..1186711 (-) 180 WP_000462851.1 aspartyl-phosphate phosphatase Spo0E family protein -
  AS54_RS06995 (AS54_1232) - 1186959..1187858 (+) 900 WP_001241732.1 fumarylacetoacetate hydrolase family protein -
  AS54_RS07000 (AS54_1233) - 1187894..1188178 (-) 285 WP_000925338.1 hypothetical protein -

Sequence

Protein


Download         Length: 1241 a.a.        Molecular weight: 142662.93 Da        Isoelectric Point: 4.8339

>NTDB_id=128474 AS54_RS06950 WP_000572292.1 1180387..1184112(+) (addA) [Bacillus cereus 03BB102]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRIETLQADLALLGTLSSAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDIVKQVDSLRNKAKDEV
KKLQEELFSRRPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSEDGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVIDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEKELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDATKEMEKWLDAREHSEWLLPDHVRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPDEIYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKDEVYDRLMWKYGYEEATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITVEVLQEQIAGMVNKELLTFEQAEEIAVEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGESGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFEQAKPIL
ETRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVIKVEE

Nucleotide


Download         Length: 3726 bp        

>NTDB_id=128474 AS54_RS06950 WP_000572292.1 1180387..1184112(+) (addA) [Bacillus cereus 03BB102]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCGGTTGTAGCGAACGGACG
TGATATTTTAGTCGCGGCAGCAGCTGGATCAGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATAAATG
AAGAAAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACAAATGCAGCAGCGCAAGAGATGAAAAACAGAATT
GGGGAAGCGTTAGAAAAAGTATTAATTGATGAACCAGGATCTCAGCACGTAAGAAAGCAACTGAGCTTATTAAATAAAGC
TTCCATTTCAACGATCCATTCATTTTGTTTACAAGTTATTAGAGGATATTATTACATGCTTGATGTTGATCCTCGTTTCC
GCATTGCGAATCAAACAGAAAATGAATTATTAAAAGAAGAAGTGTTAGATGACATATTAGAAGAAGAGTATGGAATAGAA
GATAATACGATATTCTTTGAACTCGTTGATCGTTATACGAGCGACCGTAGTGATGATGATTTACAACGTATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAAAAATGGCTCGATAAATTAGTAGAAGCATATGACGTCGAAGGAA
AGACAATTGAAGATTTAGTGTACGCCTCTTACTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCGGAACAGCATATT
CGTAAAGCAACTGAGCTCGCAATGCTTCCTGACGGTCCAGCGCCTCGCATTGAAACGCTGCAAGCAGATTTAGCTTTACT
TGGAACGTTATCATCAGCTGCTCGTGAATCGTGGACAAGCGTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGCATTAAGAAAAGCGATTATAACGAGGATATTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCAAAAGATGAAGTG
AAGAAATTACAAGAAGAGTTATTTAGCCGCAGGCCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAGCTCGTTCAACTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGCATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAGTGAAGATGGTGAAATGAAGCCATCAGCAGTAGCACTTCAA
TATCGTAATAAATTTGCTGAAGTATTAGTCGATGAATATCAAGATACGAACTTCGTACAAGAATCAATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTATTCATGGTTGGTGACGTGAAGCAGTCGATTTATCGTTTCCGACTAGCAG
AACCAGGATTATTCTTAGGAAAGTATAAACGTTTCACACAAGAAGGATTAGGCGGCGGAATGAAAATTGACTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTGTTAGCGGGTACAAACTTTATCTTCAAACAAATTATGGGCGAAGAAGTTGGGGAAAT
TGATTACGATGCTGACGCTGAATTAAAGCTAGGTGCTAGCTATCCAGAAGGTGAAGATGTAGCAGCTGAACTATTGTGCA
TTCAGCAAACAGAAGAAGAAGTAATAGACGGTGAAGAAGGTGCGGAAGTAGAAAAGGCACAGCTTGAAGCACGTCTTATG
GCGCAGCGCATTAAAGCGATGGTTGATTCAGGTTATGAAGTGTATGATCGTAAAACGGATAGTATGCGCCCTGTACAATA
CCGTGACTTCGTTATTTTACTTCGCTCTATGCCGTGGGCACCGCAAATTATGGAAGAGTTAAAGTTGCAAGGAATTCCAG
TATACGCTGATCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATAATGATGAACGTATTCCGCGTGATTGATAAT
CCGATGCAAGATATTCCGCTTGCAGCAGTACTTCGTTCTCCAATCGTTGGATTAAATGATGAAGAACTTGCAACGCTTCG
TGCTCACGGAAAGAAAGGCTCGTTTTATGAAGTGATGAGCTCATTCTTAAAAGGAGCACCGCTTGAAGAAGAAAAAGAAC
TCCATGATAAATTAGAGTGGTTCTATAACTTACTACAAGGATGGCGTGAATTCGCGCGTCAACAGTCTCTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTACGACTTTGTCGGTGGTTTACCAGCTGGAAAGCAAAGACAAGCAAATTT
GCGTGTATTATATGACCGCGCAAGACAATATGAGGCAACATCGTTTAGAGGATTATTCCGTTTCTTACGCTTTATTGAGC
GTATTTTAGAACGCGGTGACGATATGGGTACGGCGAGAGCTTTAGGTGAACAAGAAGATGTCGTTCGCATTATGACAATT
CATAAAAGTAAAGGACTTGAGTTCCCAGTCGTATTTGTCGCTGGACTTGGTCGTCGTTTTAATACACAAGATTTAATGAA
ACGTTTCTTACTGCATAAAGACTTCGGTTTCGGTTCGCAATTTATCGATCCTCGTAAACGAATTAAATATACGACATTAT
CACAACTTGCAATTAAGCGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTCTTATACGTAGCGTTAACACGG
GCAAAAGAGAAGTTAATTTTAATTGGAACGGTTAAGGATGCAACTAAGGAAATGGAAAAATGGCTGGATGCGAGGGAGCA
TAGTGAATGGTTATTACCAGATCACGTACGTGCCGGAGCATCTTGTTATTTAGACTGGATTGCACCTTCCTTATATAGAC
ACCGTGATAGTGAAATGCTTCTTGAATTAGGGCAAGGAAGTATTCCAGATGAAATTTATGGGTATGACACTAGCTGGAAA
GTAGAAGTTGTTGACGGGAACACGTTACTTGCGCCAGAACCCGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCACTTCG
TGAGAAAAAAGCTGTTCCCCTGCAAAGTGAACGAAAAGATGAAGTGTACGACAGGTTAATGTGGAAGTACGGATATGAGG
AAGCGACATCTCATCGTGCGAAGCAATCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAACGCT
TTTATTAAAAAATTACGTGCACCAATTAAAACACGTCCTCGTTTTATGGAGAAAAAAGGGTTAACGTACGCAGAGCGAGG
AACAGCAGTCCATGCCGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACAGTTGAAGTTCTTCAAGAGCAAATTGCTG
GAATGGTAAATAAAGAATTATTAACATTTGAACAAGCAGAAGAAATAGCAGTTGAAAAAGTGATTTCATTCTTTGACAGT
GACCTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAAGC
GTATCAAGATTGGCAAGGGGAGAGCGGGGAATCAATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGATG
GTATTACTTTAATCGACTTTAAAACGGATACGATTGAAGGGAAGTTCCCGGGAGGATTCGAACAAGCGAAACCAATTTTA
GAAACTCGTTACAAAGTGCAGCTTTCGTTATATGCAAAGGCACTTGAGAAAAGCTTACAACATCCTGTGAAAGAGAAATG
TTTATACTTCTTTGATGGTAATCATGTTATAAAAGTTGAAGAATAG

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(476-890)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(14-465)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1060-1232)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0RAX7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

53.328

100

0.536

Multiple sequence alignment