Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   AW22_RS24310 Genome accession   NZ_CP009300
Coordinates   4425809..4427185 (-) Length   458 a.a.
NCBI ID   WP_001085212.1    Uniprot ID   A0A2B3Y2M8
Organism   Bacillus cereus D17     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4420809..4432185
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AW22_RS24290 (AW22_4609) ispF 4422174..4422650 (-) 477 WP_000488386.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  AW22_RS24295 (AW22_4610) ispD 4422765..4423445 (-) 681 WP_000288292.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  AW22_RS24300 (AW22_4611) - 4423462..4424571 (-) 1110 WP_000919684.1 PIN/TRAM domain-containing protein -
  AW22_RS24305 (AW22_4612) disA 4424732..4425805 (-) 1074 WP_000392168.1 DNA integrity scanning diadenylate cyclase DisA -
  AW22_RS24310 (AW22_4613) radA 4425809..4427185 (-) 1377 WP_001085212.1 DNA repair protein RadA Machinery gene
  AW22_RS24315 (AW22_4614) clpC 4427282..4429717 (-) 2436 WP_000971179.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  AW22_RS24320 (AW22_4615) - 4429740..4430804 (-) 1065 WP_000050832.1 protein arginine kinase -
  AW22_RS24325 (AW22_4616) - 4430809..4431357 (-) 549 WP_000128391.1 UvrB/UvrC motif-containing protein -
  AW22_RS24330 (AW22_4617) ctsR 4431531..4431992 (-) 462 WP_001244563.1 transcriptional regulator CtsR -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49858.44 Da        Isoelectric Point: 7.1421

>NTDB_id=128375 AW22_RS24310 WP_001085212.1 4425809..4427185(-) (radA) [Bacillus cereus D17]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQTEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVNGSNLFVVSETDLQRIA
THIEEVNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=128375 AW22_RS24310 WP_001085212.1 4425809..4427185(-) (radA) [Bacillus cereus D17]
ATGGCTAAAAAGAAAACAAAATTCACATGTCAAGAGTGTGGTTATCAGTCACCAAAATATATGGGGAAATGTCCTGGATG
TGGTCAATGGAATACGCTTGTTGAAGAGATGGAACCAGTTGTATCATCAAGACGCCTTAATTATGCCAATGCAATTCAAA
CAGAAGTAACAAAACCAAGGCGCTTAACAGAAGTGGAAACAAAGTCTGAGGCGCGTATTGAAACAAAATTCCAAGAATTT
AACCGTGTACTTGGTGGTGGGATTGTAGATGGATCCTTAGTACTTATTGGTGGAGACCCCGGGATTGGAAAATCAACGTT
ACTATTACAAATATCATCGCAATTAGCGGATTCTTCATATGATGTACTATATATATCGGGTGAGGAATCAGCAAAACAGA
TTAAACTGCGTGCCGATCGTCTACATGTAAATGGTAGTAATCTATTTGTTGTATCAGAGACGGATTTACAGCGAATTGCA
ACACATATTGAAGAGGTGAACCCAGCCTTTGTTGTTATTGACTCTATTCAAACAATACATTTACCTGAGGTAACATCAGC
GCCGGGGAGTGTGGCACAAGTTCGTGAATGTACAGCGGAATTAATGAAACTTGCAAAAACGAAAGGAATCCCTATTTTTA
TCGTTGGACATGTGACAAAAGAGGGAGCAATTGCAGGACCTCGTATGTTAGAACATATGGTCGATGCAGTTCTTTATTTT
GAAGGAGACCGTCATCATACATACCGTATTTTGCGAGCAGTAAAGAATCGCTTTGGTTCTACGAATGAAATGGGTATTTT
TGAAATGAAAGAACTTGGTCTTGCAGAAGTATTAAACCCTTCTGAGATTTTCCTTGAGGAAAGACCAGTGGGGGTTGCGG
GATCAACAGTGGTTGCTTCAATGGAAGGAACAAGACCAGTTTTAGTAGAAATACAAGCATTAATCTCCCCTACTAGTTTT
GGAAACCCTCGAAGAATGGCAACGGGAATTGATCATAACCGTGTATCGCTTATTATGGCGGTGTTAGAAAAAAGAACAGG
CTTATTATTGCAAAATCAGGACGCATATTTAAAAGTAGCAGGTGGTTTGAAGTTAGATGAACCAGCTATTGATTTAGCTG
TCGCTTTAAGTATAGCTTCAAGTTTCAGAGATAAATCTACAGCACCAACCGATGCAGTAATAGGAGAAGTAGGATTAACT
GGAGAAATAAGAAGAGTATCAAGAATTGAACAACGTGTACAAGAAGCAGCTAAATTAGGATTTCAACGTGCTATTATTCC
TAGAAAAAATTTGGGAGGATGGACAATTCCGGATGGGATTGAGGTAGTTGGTGTATCTAATTTAGGAGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(60-222)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2B3Y2M8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.946

100

0.751
  radA Streptococcus mitis NCTC 12261

63.797

98.908

0.631
  radA Streptococcus pneumoniae Rx1

63.576

98.908

0.629
  radA Streptococcus pneumoniae D39

63.576

98.908

0.629
  radA Streptococcus pneumoniae R6

63.576

98.908

0.629
  radA Streptococcus pneumoniae TIGR4

63.576

98.908

0.629
  radA Streptococcus mitis SK321

63.576

98.908

0.629

Multiple sequence alignment